Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients

Human T-cell leukemia virus type 1 (HTLV-1) is associated with an increased risk of tuberculosis (TB). This study aimed to evaluate the performance of the QuantiFERON-TB Gold (QFT) test for the diagnosis of <i>Mycobacterium tuberculosis</i> (MTB) infection in HTLV-1-infected individuals....

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Main Authors: Luana Leandro Gois, Natália Barbosa Carvalho, Fred Luciano Neves Santos, Carlos Gustavo Regis-Silva, Thainá Gonçalves Tolentino Figueiredo, Bernardo Galvão-Castro, Edgar Marcelino Carvalho, Maria Fernanda Rios Grassi
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Language:English
Published: MDPI AG 2024-11-01
Series:Viruses
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Online Access:https://www.mdpi.com/1999-4915/16/12/1873
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author Luana Leandro Gois
Natália Barbosa Carvalho
Fred Luciano Neves Santos
Carlos Gustavo Regis-Silva
Thainá Gonçalves Tolentino Figueiredo
Bernardo Galvão-Castro
Edgar Marcelino Carvalho
Maria Fernanda Rios Grassi
author_facet Luana Leandro Gois
Natália Barbosa Carvalho
Fred Luciano Neves Santos
Carlos Gustavo Regis-Silva
Thainá Gonçalves Tolentino Figueiredo
Bernardo Galvão-Castro
Edgar Marcelino Carvalho
Maria Fernanda Rios Grassi
author_sort Luana Leandro Gois
collection DOAJ
description Human T-cell leukemia virus type 1 (HTLV-1) is associated with an increased risk of tuberculosis (TB). This study aimed to evaluate the performance of the QuantiFERON-TB Gold (QFT) test for the diagnosis of <i>Mycobacterium tuberculosis</i> (MTB) infection in HTLV-1-infected individuals. HTLV-1-infected participants were divided into four groups: HTLV-1-infected individuals with a history of tuberculosis (HTLV/TB), individuals with positive HTLV and tuberculin skin tests (HTLV/TST+) or negative TST (HTLV/TST−), and HTLV-1-negative individuals with positive TST results (HN/TST+). We compared the diagnostic performance of the QFT assay with that of the TST as a reference and evaluated test sensitivity, specificity, accuracy, likelihood ratio, and diagnostic odds ratio. The results showed a higher frequency of positive TST results and induration diameter ≥10 mm in HTLV-1-infected individuals than in the controls. The QFT test was more frequently positive in the HTLV/TB group than in the other groups, while a combined analysis of HTLV/TB and HTLV/TST+ indicated a QFT sensitivity of 57.5%. No significant differences were found in the other diagnostic performance measures, as QFT test results were in agreement with TST results, particularly in TST-negative individuals. Given the low sensitivity of QFT for LTBI in individuals infected with HTLV-1, the TST may be preferable in regions where both infections are endemic.
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spelling doaj-art-e6a3bcd2c3e94e64b27e0cf6d90536be2025-08-20T02:43:23ZengMDPI AGViruses1999-49152024-11-011612187310.3390/v16121873Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected PatientsLuana Leandro Gois0Natália Barbosa Carvalho1Fred Luciano Neves Santos2Carlos Gustavo Regis-Silva3Thainá Gonçalves Tolentino Figueiredo4Bernardo Galvão-Castro5Edgar Marcelino Carvalho6Maria Fernanda Rios Grassi7Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador 40231-300, Bahia, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador 40110-060, Bahia, BrazilLaboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-BA), Salvador 40296-710, Bahia, BrazilLaboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-BA), Salvador 40296-710, Bahia, BrazilEscola Bahiana de Medicina e Saúde Pública, Salvador 40290-000, Bahia, BrazilLaboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-BA), Salvador 40296-710, Bahia, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador 40110-060, Bahia, BrazilLaboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-BA), Salvador 40296-710, Bahia, BrazilHuman T-cell leukemia virus type 1 (HTLV-1) is associated with an increased risk of tuberculosis (TB). This study aimed to evaluate the performance of the QuantiFERON-TB Gold (QFT) test for the diagnosis of <i>Mycobacterium tuberculosis</i> (MTB) infection in HTLV-1-infected individuals. HTLV-1-infected participants were divided into four groups: HTLV-1-infected individuals with a history of tuberculosis (HTLV/TB), individuals with positive HTLV and tuberculin skin tests (HTLV/TST+) or negative TST (HTLV/TST−), and HTLV-1-negative individuals with positive TST results (HN/TST+). We compared the diagnostic performance of the QFT assay with that of the TST as a reference and evaluated test sensitivity, specificity, accuracy, likelihood ratio, and diagnostic odds ratio. The results showed a higher frequency of positive TST results and induration diameter ≥10 mm in HTLV-1-infected individuals than in the controls. The QFT test was more frequently positive in the HTLV/TB group than in the other groups, while a combined analysis of HTLV/TB and HTLV/TST+ indicated a QFT sensitivity of 57.5%. No significant differences were found in the other diagnostic performance measures, as QFT test results were in agreement with TST results, particularly in TST-negative individuals. Given the low sensitivity of QFT for LTBI in individuals infected with HTLV-1, the TST may be preferable in regions where both infections are endemic.https://www.mdpi.com/1999-4915/16/12/1873HTLV-1tuberculosistuberculin skin testQuantiFERON
spellingShingle Luana Leandro Gois
Natália Barbosa Carvalho
Fred Luciano Neves Santos
Carlos Gustavo Regis-Silva
Thainá Gonçalves Tolentino Figueiredo
Bernardo Galvão-Castro
Edgar Marcelino Carvalho
Maria Fernanda Rios Grassi
Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients
Viruses
HTLV-1
tuberculosis
tuberculin skin test
QuantiFERON
title Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients
title_full Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients
title_fullStr Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients
title_full_unstemmed Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients
title_short Evaluation of QuantiFERON-TB Gold for the Diagnosis of <i>Mycobacterium tuberculosis</i> Infection in HTLV-1-Infected Patients
title_sort evaluation of quantiferon tb gold for the diagnosis of i mycobacterium tuberculosis i infection in htlv 1 infected patients
topic HTLV-1
tuberculosis
tuberculin skin test
QuantiFERON
url https://www.mdpi.com/1999-4915/16/12/1873
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