CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.

CD200 receptor (CD200R) negatively regulates peripheral and mucosal innate immune responses. Viruses, including herpesviruses, have acquired functional CD200 orthologs, implying that viral exploitation of this pathway is evolutionary advantageous. However, the role that CD200R signaling plays during...

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Main Authors: Gabrielle Stack, Emma Jones, Morgan Marsden, Maria A Stacey, Robert J Snelgrove, Paul Lacaze, Laura C Jacques, Simone M Cuff, Richard J Stanton, Awen M Gallimore, Tracy Hussell, Gavin W G Wilkinson, Peter Ghazal, Philip R Taylor, Ian R Humphreys
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-02-01
Series:PLoS Pathogens
Online Access:https://doi.org/10.1371/journal.ppat.1004641
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author Gabrielle Stack
Emma Jones
Morgan Marsden
Maria A Stacey
Robert J Snelgrove
Paul Lacaze
Laura C Jacques
Simone M Cuff
Richard J Stanton
Awen M Gallimore
Tracy Hussell
Gavin W G Wilkinson
Peter Ghazal
Philip R Taylor
Ian R Humphreys
author_facet Gabrielle Stack
Emma Jones
Morgan Marsden
Maria A Stacey
Robert J Snelgrove
Paul Lacaze
Laura C Jacques
Simone M Cuff
Richard J Stanton
Awen M Gallimore
Tracy Hussell
Gavin W G Wilkinson
Peter Ghazal
Philip R Taylor
Ian R Humphreys
author_sort Gabrielle Stack
collection DOAJ
description CD200 receptor (CD200R) negatively regulates peripheral and mucosal innate immune responses. Viruses, including herpesviruses, have acquired functional CD200 orthologs, implying that viral exploitation of this pathway is evolutionary advantageous. However, the role that CD200R signaling plays during herpesvirus infection in vivo requires clarification. Utilizing the murine cytomegalovirus (MCMV) model, we demonstrate that CD200R facilitates virus persistence within mucosal tissue. Specifically, MCMV infection of CD200R-deficient mice (CD200R(-/-)) elicited heightened mucosal virus-specific CD4 T cell responses that restricted virus persistence in the salivary glands. CD200R did not directly inhibit lymphocyte effector function. Instead, CD200R(-/-) mice exhibited enhanced APC accumulation that in the mucosa was a consequence of elevated cellular proliferation. Although MCMV does not encode an obvious CD200 homolog, productive replication in macrophages induced expression of cellular CD200. CD200 from hematopoietic and non-hematopoietic cells contributed independently to suppression of antiviral control in vivo. These results highlight the CD200-CD200R pathway as an important regulator of antiviral immunity during cytomegalovirus infection that is exploited by MCMV to establish chronicity within mucosal tissue.
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spelling doaj-art-e69f5ed2db144e32af2dee37e2b1a0ca2025-08-20T03:10:05ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742015-02-01112e100464110.1371/journal.ppat.1004641CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.Gabrielle StackEmma JonesMorgan MarsdenMaria A StaceyRobert J SnelgrovePaul LacazeLaura C JacquesSimone M CuffRichard J StantonAwen M GallimoreTracy HussellGavin W G WilkinsonPeter GhazalPhilip R TaylorIan R HumphreysCD200 receptor (CD200R) negatively regulates peripheral and mucosal innate immune responses. Viruses, including herpesviruses, have acquired functional CD200 orthologs, implying that viral exploitation of this pathway is evolutionary advantageous. However, the role that CD200R signaling plays during herpesvirus infection in vivo requires clarification. Utilizing the murine cytomegalovirus (MCMV) model, we demonstrate that CD200R facilitates virus persistence within mucosal tissue. Specifically, MCMV infection of CD200R-deficient mice (CD200R(-/-)) elicited heightened mucosal virus-specific CD4 T cell responses that restricted virus persistence in the salivary glands. CD200R did not directly inhibit lymphocyte effector function. Instead, CD200R(-/-) mice exhibited enhanced APC accumulation that in the mucosa was a consequence of elevated cellular proliferation. Although MCMV does not encode an obvious CD200 homolog, productive replication in macrophages induced expression of cellular CD200. CD200 from hematopoietic and non-hematopoietic cells contributed independently to suppression of antiviral control in vivo. These results highlight the CD200-CD200R pathway as an important regulator of antiviral immunity during cytomegalovirus infection that is exploited by MCMV to establish chronicity within mucosal tissue.https://doi.org/10.1371/journal.ppat.1004641
spellingShingle Gabrielle Stack
Emma Jones
Morgan Marsden
Maria A Stacey
Robert J Snelgrove
Paul Lacaze
Laura C Jacques
Simone M Cuff
Richard J Stanton
Awen M Gallimore
Tracy Hussell
Gavin W G Wilkinson
Peter Ghazal
Philip R Taylor
Ian R Humphreys
CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.
PLoS Pathogens
title CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.
title_full CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.
title_fullStr CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.
title_full_unstemmed CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.
title_short CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue.
title_sort cd200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue
url https://doi.org/10.1371/journal.ppat.1004641
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