Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats
We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that b...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/9458396 |
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| author | Bo Wei Chengshuo Huang Mingyan Zhao Peng Li Xiang Gao Junchao Kong Yanru Niu Rui Huang Juanhua Quan Jinsong Wei Jiaqi Chu |
| author_facet | Bo Wei Chengshuo Huang Mingyan Zhao Peng Li Xiang Gao Junchao Kong Yanru Niu Rui Huang Juanhua Quan Jinsong Wei Jiaqi Chu |
| author_sort | Bo Wei |
| collection | DOAJ |
| description | We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results. We also assessed changes to bone trabeculae in the proximal tibial growth plate. In ovariectomized rats treated with PRP or with a combination of PRP and BMSCs, the trabecular connectivity densities (Conn.D), bone volume ratios (BV/TV), and numbers (Tb.N) in the defect areas increased significantly from day 7 to day 42. These results indicate that PRP treatment enhances bone microarchitecture in osteoporosis. Moreover, expression levels of osteogenesis-specific marker genes including RUNX2, OSX, and OPN were significantly upregulated in rats treated with PRP and BMSCs compared to those of other groups. Thus, we conclude that treatment with PRP combined with BMSCs significantly promotes healing of osteoporotic bone defects. This study provides an alternative strategy for the treatment of osteoporotic bone loss. |
| format | Article |
| id | doaj-art-e699f77f0f4f42e2be9e905b463e5d9c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-e699f77f0f4f42e2be9e905b463e5d9c2025-02-03T00:59:54ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/94583969458396Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized RatsBo Wei0Chengshuo Huang1Mingyan Zhao2Peng Li3Xiang Gao4Junchao Kong5Yanru Niu6Rui Huang7Juanhua Quan8Jinsong Wei9Jiaqi Chu10Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Gastroenterology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaWe evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results. We also assessed changes to bone trabeculae in the proximal tibial growth plate. In ovariectomized rats treated with PRP or with a combination of PRP and BMSCs, the trabecular connectivity densities (Conn.D), bone volume ratios (BV/TV), and numbers (Tb.N) in the defect areas increased significantly from day 7 to day 42. These results indicate that PRP treatment enhances bone microarchitecture in osteoporosis. Moreover, expression levels of osteogenesis-specific marker genes including RUNX2, OSX, and OPN were significantly upregulated in rats treated with PRP and BMSCs compared to those of other groups. Thus, we conclude that treatment with PRP combined with BMSCs significantly promotes healing of osteoporotic bone defects. This study provides an alternative strategy for the treatment of osteoporotic bone loss.http://dx.doi.org/10.1155/2016/9458396 |
| spellingShingle | Bo Wei Chengshuo Huang Mingyan Zhao Peng Li Xiang Gao Junchao Kong Yanru Niu Rui Huang Juanhua Quan Jinsong Wei Jiaqi Chu Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats Stem Cells International |
| title | Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats |
| title_full | Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats |
| title_fullStr | Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats |
| title_full_unstemmed | Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats |
| title_short | Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats |
| title_sort | effect of mesenchymal stem cells and platelet rich plasma on the bone healing of ovariectomized rats |
| url | http://dx.doi.org/10.1155/2016/9458396 |
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