Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study
<b>Background/Objectives:</b> Tooth discoloration is a common concern associated with the use of intra-canal medicaments, particularly those containing antibiotics. This study aims to evaluate the tooth discoloration potential of an experimental antibiotic paste modified with Nano Chitos...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Dentistry Journal |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6767/13/7/307 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849733317568495616 |
|---|---|
| author | Mohamed Ahmed Elsayed Md Sofiqul Islam Safiya Ali Zainab Hussain Muhammed Mustahsen Rahman Okba Mahmoud |
| author_facet | Mohamed Ahmed Elsayed Md Sofiqul Islam Safiya Ali Zainab Hussain Muhammed Mustahsen Rahman Okba Mahmoud |
| author_sort | Mohamed Ahmed Elsayed |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Tooth discoloration is a common concern associated with the use of intra-canal medicaments, particularly those containing antibiotics. This study aims to evaluate the tooth discoloration potential of an experimental antibiotic paste modified with Nano Chitosan (APNC) and compare it with two antibiotic pastes and two calcium hydroxide-based pastes over different time intervals. <b>Methods</b>: Fifty bovine incisors were standardized and prepared up to size 60. The teeth were randomly assigned into five groups based on the medicament applied: Metapaste, Metapex, modified Triple Antibiotic Paste (mTAP), Double Antibiotic Paste (DAP), and APNC. A digital spectrophotometer was used to measure the color parameters (L*, a*, and b*) at two zones, above and below the cemento-enamel junction (CEJ), across four-time points: before application (T0- baseline), immediately after application (T1), after two weeks (T2), and after one month (T3). The color changes (ΔE) were calculated and statistically analyzed using repeated-measure ANOVA. <b>Results</b>: Statistically significant differences in discoloration were observed between the tested medicaments after one month (<i>p</i> < 0.05). mTAP caused the highest ΔE values both above and below the CEJ at all time points, particularly after one month (<i>p</i> < 0.05). Conversely, APNC, Metapaste, and DAP demonstrated the least discoloration, with no significant differences among them. The degree of discoloration was time-dependent and more pronounced below the CEJ in all groups. <b>Conclusions</b>: After one month, the experimental APNC paste induced tooth discoloration comparable to that of Metapaste, indicating minimal esthetic compromise. APNC may be a promising alternative to traditional antibiotic pastes with minimal discoloration effects. |
| format | Article |
| id | doaj-art-e698b8b3ad5c483fadda3689830497bb |
| institution | DOAJ |
| issn | 2304-6767 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj-art-e698b8b3ad5c483fadda3689830497bb2025-08-20T03:08:05ZengMDPI AGDentistry Journal2304-67672025-07-0113730710.3390/dj13070307Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro StudyMohamed Ahmed Elsayed0Md Sofiqul Islam1Safiya Ali2Zainab Hussain3Muhammed Mustahsen Rahman4Okba Mahmoud5Department of Endodontics, RAK College of Dental Sciences, RAK Medical and Health Sciences University, Ras Al-Khaimah P.O. Box 12973, United Arab EmiratesDepartment of Operative Dentistry, RAK College of Dental Sciences, RAK Medical and Health Sciences University, Ras Al-Khaimah P.O. Box 12973, United Arab EmiratesDepartment of Clinical Science, RAK College of Dental Sciences, RAK Medical and Health Sciences University, Ras Al-Khaimah P.O. Box 12973, United Arab EmiratesDepartment of Clinical Science, RAK College of Dental Sciences, RAK Medical and Health Sciences University, Ras Al-Khaimah P.O. Box 12973, United Arab EmiratesDepartment of Periodontology, RAK College of Dental Sciences, RAK Medical and Health Sciences University, Ras Al-Khaimah, P.O. Box 12973, United Arab EmiratesDepartment of Clinical Sciences, College of Dentistry, Ajman University, Ajman P.O. Box 346, United Arab Emirates<b>Background/Objectives:</b> Tooth discoloration is a common concern associated with the use of intra-canal medicaments, particularly those containing antibiotics. This study aims to evaluate the tooth discoloration potential of an experimental antibiotic paste modified with Nano Chitosan (APNC) and compare it with two antibiotic pastes and two calcium hydroxide-based pastes over different time intervals. <b>Methods</b>: Fifty bovine incisors were standardized and prepared up to size 60. The teeth were randomly assigned into five groups based on the medicament applied: Metapaste, Metapex, modified Triple Antibiotic Paste (mTAP), Double Antibiotic Paste (DAP), and APNC. A digital spectrophotometer was used to measure the color parameters (L*, a*, and b*) at two zones, above and below the cemento-enamel junction (CEJ), across four-time points: before application (T0- baseline), immediately after application (T1), after two weeks (T2), and after one month (T3). The color changes (ΔE) were calculated and statistically analyzed using repeated-measure ANOVA. <b>Results</b>: Statistically significant differences in discoloration were observed between the tested medicaments after one month (<i>p</i> < 0.05). mTAP caused the highest ΔE values both above and below the CEJ at all time points, particularly after one month (<i>p</i> < 0.05). Conversely, APNC, Metapaste, and DAP demonstrated the least discoloration, with no significant differences among them. The degree of discoloration was time-dependent and more pronounced below the CEJ in all groups. <b>Conclusions</b>: After one month, the experimental APNC paste induced tooth discoloration comparable to that of Metapaste, indicating minimal esthetic compromise. APNC may be a promising alternative to traditional antibiotic pastes with minimal discoloration effects.https://www.mdpi.com/2304-6767/13/7/307calcium hydroxideintracanal medicamentsnano chitosanspectrophotometric analysistriple antibiotic pastetooth discoloration |
| spellingShingle | Mohamed Ahmed Elsayed Md Sofiqul Islam Safiya Ali Zainab Hussain Muhammed Mustahsen Rahman Okba Mahmoud Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study Dentistry Journal calcium hydroxide intracanal medicaments nano chitosan spectrophotometric analysis triple antibiotic paste tooth discoloration |
| title | Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study |
| title_full | Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study |
| title_fullStr | Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study |
| title_full_unstemmed | Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study |
| title_short | Comparative Evaluation of Tooth Discoloration Induced by an Experimental Antibiotic Paste Modified with Nano Chitosan: An In Vitro Study |
| title_sort | comparative evaluation of tooth discoloration induced by an experimental antibiotic paste modified with nano chitosan an in vitro study |
| topic | calcium hydroxide intracanal medicaments nano chitosan spectrophotometric analysis triple antibiotic paste tooth discoloration |
| url | https://www.mdpi.com/2304-6767/13/7/307 |
| work_keys_str_mv | AT mohamedahmedelsayed comparativeevaluationoftoothdiscolorationinducedbyanexperimentalantibioticpastemodifiedwithnanochitosananinvitrostudy AT mdsofiqulislam comparativeevaluationoftoothdiscolorationinducedbyanexperimentalantibioticpastemodifiedwithnanochitosananinvitrostudy AT safiyaali comparativeevaluationoftoothdiscolorationinducedbyanexperimentalantibioticpastemodifiedwithnanochitosananinvitrostudy AT zainabhussain comparativeevaluationoftoothdiscolorationinducedbyanexperimentalantibioticpastemodifiedwithnanochitosananinvitrostudy AT muhammedmustahsenrahman comparativeevaluationoftoothdiscolorationinducedbyanexperimentalantibioticpastemodifiedwithnanochitosananinvitrostudy AT okbamahmoud comparativeevaluationoftoothdiscolorationinducedbyanexperimentalantibioticpastemodifiedwithnanochitosananinvitrostudy |