Silver Nanostructures for Determination of FKBP12 Protein
FKBP12 is a peptidyl––prolyl cis–trans isomerase that was recently proposed as a candidate biomarker for cancer, for neurodegenerations and for anti–rejection therapy after organ transplant. We designed and fabricated a nanosensor platform for the rapid and efficient determination of FKBP12 concentr...
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MDPI AG
2024-11-01
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| Series: | Engineering Proceedings |
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| author | Cosimo Bartolini Martina Tozzetti Stefano Menichetti Gabriella Caminati |
| author_facet | Cosimo Bartolini Martina Tozzetti Stefano Menichetti Gabriella Caminati |
| author_sort | Cosimo Bartolini |
| collection | DOAJ |
| description | FKBP12 is a peptidyl––prolyl cis–trans isomerase that was recently proposed as a candidate biomarker for cancer, for neurodegenerations and for anti–rejection therapy after organ transplant. We designed and fabricated a nanosensor platform for the rapid and efficient determination of FKBP12 concentration in biological fluids exploiting anisotropic silver nanoparticles (AgNps) to enhance the capabilities of Quartz Crystal Microbalance (QCM) detection. To this end, the QCM sensor was coated with a compact array of AgNPs that were further functionalized with a Self–Assembled–Monolayer containing a synthetic receptor, GPS–SH1, designed and synthesized specifically to selectively bind FKBP12. Silver nanoflowers, AgNFs, and silver dendrites, AgNDs, were prepared by electrodeposition and characterized by means of UV–Vis spectroscopy, Scanning Electron Microscopy (SEM), QCM and water contact angle (CA). The AgNPs@Au/GPS–SH1–functionalized QCM sensors were used to detect increasing concentrations of FKBP12 in solution; the results showed that the use of AgNDs significantly enhanced the sensitivity of the sensor with respect to flat Ag sensor chips, allowing the detection of FKBP12 at sub–picomolar concentrations. |
| format | Article |
| id | doaj-art-e68e07bd871f48aabce301eab8c63ed0 |
| institution | DOAJ |
| issn | 2673-4591 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Engineering Proceedings |
| spelling | doaj-art-e68e07bd871f48aabce301eab8c63ed02025-08-20T02:53:33ZengMDPI AGEngineering Proceedings2673-45912024-11-01731910.3390/engproc2024073009Silver Nanostructures for Determination of FKBP12 ProteinCosimo Bartolini0Martina Tozzetti1Stefano Menichetti2Gabriella Caminati3Department of Chemistry, University of Florence, Via della Lastruccia 3–13, 50019 Sesto Fiorentino, FI, ItalyDepartment of Chemistry, University of Florence, Via della Lastruccia 3–13, 50019 Sesto Fiorentino, FI, ItalyDepartment of Chemistry, University of Florence, Via della Lastruccia 3–13, 50019 Sesto Fiorentino, FI, ItalyDepartment of Chemistry, University of Florence, Via della Lastruccia 3–13, 50019 Sesto Fiorentino, FI, ItalyFKBP12 is a peptidyl––prolyl cis–trans isomerase that was recently proposed as a candidate biomarker for cancer, for neurodegenerations and for anti–rejection therapy after organ transplant. We designed and fabricated a nanosensor platform for the rapid and efficient determination of FKBP12 concentration in biological fluids exploiting anisotropic silver nanoparticles (AgNps) to enhance the capabilities of Quartz Crystal Microbalance (QCM) detection. To this end, the QCM sensor was coated with a compact array of AgNPs that were further functionalized with a Self–Assembled–Monolayer containing a synthetic receptor, GPS–SH1, designed and synthesized specifically to selectively bind FKBP12. Silver nanoflowers, AgNFs, and silver dendrites, AgNDs, were prepared by electrodeposition and characterized by means of UV–Vis spectroscopy, Scanning Electron Microscopy (SEM), QCM and water contact angle (CA). The AgNPs@Au/GPS–SH1–functionalized QCM sensors were used to detect increasing concentrations of FKBP12 in solution; the results showed that the use of AgNDs significantly enhanced the sensitivity of the sensor with respect to flat Ag sensor chips, allowing the detection of FKBP12 at sub–picomolar concentrations.https://www.mdpi.com/2673-4591/73/1/9FKBP12nanosensorssilver nanostructuresneurodegenerative diseasescancertransplant rejection |
| spellingShingle | Cosimo Bartolini Martina Tozzetti Stefano Menichetti Gabriella Caminati Silver Nanostructures for Determination of FKBP12 Protein Engineering Proceedings FKBP12 nanosensors silver nanostructures neurodegenerative diseases cancer transplant rejection |
| title | Silver Nanostructures for Determination of FKBP12 Protein |
| title_full | Silver Nanostructures for Determination of FKBP12 Protein |
| title_fullStr | Silver Nanostructures for Determination of FKBP12 Protein |
| title_full_unstemmed | Silver Nanostructures for Determination of FKBP12 Protein |
| title_short | Silver Nanostructures for Determination of FKBP12 Protein |
| title_sort | silver nanostructures for determination of fkbp12 protein |
| topic | FKBP12 nanosensors silver nanostructures neurodegenerative diseases cancer transplant rejection |
| url | https://www.mdpi.com/2673-4591/73/1/9 |
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