Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event
Coronary artery disease (CAD) is a prevalent chronic inflammatory cardiac disorder. An early diagnosis is likely to help in the prevention and proper management of this disease. As the study of proteomics provides the potential markers for detection of a disease, in the present investigation, attemp...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/3429541 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849467880675999744 |
|---|---|
| author | Monu Rupsi Kharb Ankita Sharma Monu Kumar Chaddar Rakesh Yadav Prachi Agnihotri Anand Kar Sagarika Biswas |
| author_facet | Monu Rupsi Kharb Ankita Sharma Monu Kumar Chaddar Rakesh Yadav Prachi Agnihotri Anand Kar Sagarika Biswas |
| author_sort | Monu |
| collection | DOAJ |
| description | Coronary artery disease (CAD) is a prevalent chronic inflammatory cardiac disorder. An early diagnosis is likely to help in the prevention and proper management of this disease. As the study of proteomics provides the potential markers for detection of a disease, in the present investigation, attempt has been made to identify disease-associated differential proteins involved in CAD pathogenesis. For this study, a total of 200 selected CAD patients were considered, who were recruited for percutaneous coronary intervention (PCI) treatment. The proteomic analysis was performed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF MS/MS. Samples were also subjected to Western blot analysis, enzyme-linked immunosorbent assay (ELISA), peripheral blood mononuclear cells isolation immunofluorescence (IF) analysis, analytical screening by fluorescence-activated cell sorting (FACS), and in silico analysis. The representative data were shown as mean±SD of at least three experiments. A total of 19 proteins were identified. Among them, the most abundant five proteins (serotransferrin, talin-1, alpha-2HS glycoprotein, transthyretin (TTR), fibrinogen-α chain) were found to have altered level in CAD. Serotransferrin, talin-1, alpha-2HS glycoprotein, and transthyretin (TTR) were found to have lower level, whereas fibrinogen-α chain was found to have higher level in CAD plasma compared to healthy, confirmed by Western blot analysis. TTR, an important acute phase transport protein, was validated low level in 200 CAD patients who confirmed to undergo PCI treatment. Further, in silico and in vitro studies of TTR indicated a downexpression of CAD in plasma as compared to the plasma of healthy individuals. Lower level of plasma TTR was determined to be an important risk marker in the atherosclerotic-approved CAD patients. We suggest that the TTR lower level predicts disease severity and hence may serve as an important marker tool for CAD screening. However, further large-scale studies are required to determine the clinical significance of TTR. |
| format | Article |
| id | doaj-art-e68b783cdb554cd89be5ca542fe5e440 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e68b783cdb554cd89be5ca542fe5e4402025-08-20T03:26:00ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/34295413429541Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important EventMonu0Rupsi Kharb1Ankita Sharma2Monu Kumar Chaddar3Rakesh Yadav4Prachi Agnihotri5Anand Kar6Sagarika Biswas7Council of Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, 110007, Delhi, IndiaCouncil of Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, 110007, Delhi, IndiaCouncil of Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, 110007, Delhi, IndiaCouncil of Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, 110007, Delhi, IndiaAll India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaCouncil of Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, 110007, Delhi, IndiaSchool of Life Sciences, Takshashila Campus, Devi Ahilya University, 452017, Indore, IndiaCouncil of Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, 110007, Delhi, IndiaCoronary artery disease (CAD) is a prevalent chronic inflammatory cardiac disorder. An early diagnosis is likely to help in the prevention and proper management of this disease. As the study of proteomics provides the potential markers for detection of a disease, in the present investigation, attempt has been made to identify disease-associated differential proteins involved in CAD pathogenesis. For this study, a total of 200 selected CAD patients were considered, who were recruited for percutaneous coronary intervention (PCI) treatment. The proteomic analysis was performed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF MS/MS. Samples were also subjected to Western blot analysis, enzyme-linked immunosorbent assay (ELISA), peripheral blood mononuclear cells isolation immunofluorescence (IF) analysis, analytical screening by fluorescence-activated cell sorting (FACS), and in silico analysis. The representative data were shown as mean±SD of at least three experiments. A total of 19 proteins were identified. Among them, the most abundant five proteins (serotransferrin, talin-1, alpha-2HS glycoprotein, transthyretin (TTR), fibrinogen-α chain) were found to have altered level in CAD. Serotransferrin, talin-1, alpha-2HS glycoprotein, and transthyretin (TTR) were found to have lower level, whereas fibrinogen-α chain was found to have higher level in CAD plasma compared to healthy, confirmed by Western blot analysis. TTR, an important acute phase transport protein, was validated low level in 200 CAD patients who confirmed to undergo PCI treatment. Further, in silico and in vitro studies of TTR indicated a downexpression of CAD in plasma as compared to the plasma of healthy individuals. Lower level of plasma TTR was determined to be an important risk marker in the atherosclerotic-approved CAD patients. We suggest that the TTR lower level predicts disease severity and hence may serve as an important marker tool for CAD screening. However, further large-scale studies are required to determine the clinical significance of TTR.http://dx.doi.org/10.1155/2020/3429541 |
| spellingShingle | Monu Rupsi Kharb Ankita Sharma Monu Kumar Chaddar Rakesh Yadav Prachi Agnihotri Anand Kar Sagarika Biswas Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event Mediators of Inflammation |
| title | Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event |
| title_full | Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event |
| title_fullStr | Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event |
| title_full_unstemmed | Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event |
| title_short | Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event |
| title_sort | plasma proteome profiling of coronary artery disease patients downregulation of transthyretin an important event |
| url | http://dx.doi.org/10.1155/2020/3429541 |
| work_keys_str_mv | AT monu plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT rupsikharb plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT ankitasharma plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT monukumarchaddar plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT rakeshyadav plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT prachiagnihotri plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT anandkar plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent AT sagarikabiswas plasmaproteomeprofilingofcoronaryarterydiseasepatientsdownregulationoftransthyretinanimportantevent |