New insights into the transcriptomic profile during the late stages of chicken embryonic development
While comprehensive transcriptomic characterization of muscle development during the hatching stage remains limited, we conducted an integrated analysis of coding and non-coding RNA profiles in the pectoral muscles of Arbor Acres (AA) broilers and TaoYuan (TY) chickens at embryonic days 17 (E17), 19...
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Elsevier
2025-11-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912500937X |
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| author | Di Zhao Yifan Shi Jiatai Deng Bolin Zhong Yuanyuan Zeng Qingyuan Ouyang Haihan Zhang Zehe Song Xi He |
| author_facet | Di Zhao Yifan Shi Jiatai Deng Bolin Zhong Yuanyuan Zeng Qingyuan Ouyang Haihan Zhang Zehe Song Xi He |
| author_sort | Di Zhao |
| collection | DOAJ |
| description | While comprehensive transcriptomic characterization of muscle development during the hatching stage remains limited, we conducted an integrated analysis of coding and non-coding RNA profiles in the pectoral muscles of Arbor Acres (AA) broilers and TaoYuan (TY) chickens at embryonic days 17 (E17), 19 (E19), and 21 (E21). Our findings revealed notable phenotypic differences: AA broilers exhibited greater embryo weight and larger muscle fiber cross-sectional areas compared to TY chickens. Across three developmental stages, we identified 4,577 differentially expressed genes (DEGs), 143 differentially expressed microRNAs (DEMs), 90 differentially expressed circRNAs (DECs), and 3,159 differentially expressed lncRNAs (DELs). By integrating weighted gene co-expression network analysis (WGCNA) analysis with differential expression profiling, we prioritized five coding genes (FOSL2, PDE4B, TRIB1, THBS1, and FBXO32) as key regulators of muscle development. Pathway enrichment analysis further revealed significant activation of the glycolysis/gluconeogenesis pathway in TY chickens, likely supporting the energy demands of shell pipping. To elucidate transcriptional regulatory mechanisms, we constructed competing endogenous RNA (ceRNA) networks based on miRanda predictions. This analysis identified four microRNAs (gga-miR-206, gga-miR-383-3p, gga-miR-449a, and gga-miR-449c-5p) that may modulate developmental genes through lncRNA and circRNA mediated sponge interactions. These ceRNA networks provide novel insights and a valuable framework for investigating the molecular regulation of embryonic muscle development in AA broilers and TY chickens. |
| format | Article |
| id | doaj-art-e686a19bb1b44714bffc4688e550c596 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e686a19bb1b44714bffc4688e550c5962025-08-25T04:13:56ZengElsevierPoultry Science0032-57912025-11-011041110569510.1016/j.psj.2025.105695New insights into the transcriptomic profile during the late stages of chicken embryonic developmentDi Zhao0Yifan Shi1Jiatai Deng2Bolin Zhong3Yuanyuan Zeng4Qingyuan Ouyang5Haihan Zhang6Zehe Song7Xi He8College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, China; Corresponding author at: No.1 Nongda Road, Furong District, Hunan Agricultural University, Changsha 410128, China.While comprehensive transcriptomic characterization of muscle development during the hatching stage remains limited, we conducted an integrated analysis of coding and non-coding RNA profiles in the pectoral muscles of Arbor Acres (AA) broilers and TaoYuan (TY) chickens at embryonic days 17 (E17), 19 (E19), and 21 (E21). Our findings revealed notable phenotypic differences: AA broilers exhibited greater embryo weight and larger muscle fiber cross-sectional areas compared to TY chickens. Across three developmental stages, we identified 4,577 differentially expressed genes (DEGs), 143 differentially expressed microRNAs (DEMs), 90 differentially expressed circRNAs (DECs), and 3,159 differentially expressed lncRNAs (DELs). By integrating weighted gene co-expression network analysis (WGCNA) analysis with differential expression profiling, we prioritized five coding genes (FOSL2, PDE4B, TRIB1, THBS1, and FBXO32) as key regulators of muscle development. Pathway enrichment analysis further revealed significant activation of the glycolysis/gluconeogenesis pathway in TY chickens, likely supporting the energy demands of shell pipping. To elucidate transcriptional regulatory mechanisms, we constructed competing endogenous RNA (ceRNA) networks based on miRanda predictions. This analysis identified four microRNAs (gga-miR-206, gga-miR-383-3p, gga-miR-449a, and gga-miR-449c-5p) that may modulate developmental genes through lncRNA and circRNA mediated sponge interactions. These ceRNA networks provide novel insights and a valuable framework for investigating the molecular regulation of embryonic muscle development in AA broilers and TY chickens.http://www.sciencedirect.com/science/article/pii/S003257912500937XChickenEmbryonic developmentTranscriptomic profileCompeting endogenous RNA |
| spellingShingle | Di Zhao Yifan Shi Jiatai Deng Bolin Zhong Yuanyuan Zeng Qingyuan Ouyang Haihan Zhang Zehe Song Xi He New insights into the transcriptomic profile during the late stages of chicken embryonic development Poultry Science Chicken Embryonic development Transcriptomic profile Competing endogenous RNA |
| title | New insights into the transcriptomic profile during the late stages of chicken embryonic development |
| title_full | New insights into the transcriptomic profile during the late stages of chicken embryonic development |
| title_fullStr | New insights into the transcriptomic profile during the late stages of chicken embryonic development |
| title_full_unstemmed | New insights into the transcriptomic profile during the late stages of chicken embryonic development |
| title_short | New insights into the transcriptomic profile during the late stages of chicken embryonic development |
| title_sort | new insights into the transcriptomic profile during the late stages of chicken embryonic development |
| topic | Chicken Embryonic development Transcriptomic profile Competing endogenous RNA |
| url | http://www.sciencedirect.com/science/article/pii/S003257912500937X |
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