Paneth cells in intestinal homeostasis and tissue injury.
Adult stem cell niches are often co-inhabited by cycling and quiescent stem cells. In the intestine, lineage tracing has identified Lgr5(+) cells as frequently cycling stem cells, whereas Bmi1(+), mTert(+), Hopx(+) and Lrig1(+) cells appear to be more quiescent. Here, we have applied a non-mutagenic...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038965&type=printable |
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| _version_ | 1849470195919224832 |
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| author | Sabrina Roth Patrick Franken Andrea Sacchetti Andreas Kremer Kurt Anderson Owen Sansom Riccardo Fodde |
| author_facet | Sabrina Roth Patrick Franken Andrea Sacchetti Andreas Kremer Kurt Anderson Owen Sansom Riccardo Fodde |
| author_sort | Sabrina Roth |
| collection | DOAJ |
| description | Adult stem cell niches are often co-inhabited by cycling and quiescent stem cells. In the intestine, lineage tracing has identified Lgr5(+) cells as frequently cycling stem cells, whereas Bmi1(+), mTert(+), Hopx(+) and Lrig1(+) cells appear to be more quiescent. Here, we have applied a non-mutagenic and cell cycle independent approach to isolate and characterize small intestinal label-retaining cells (LRCs) persisting in the lower third of the crypt of Lieberkühn for up to 100 days. LRCs do not express markers of proliferation and of enterocyte, goblet or enteroendocrine differentiation, but are positive for Paneth cell markers. While during homeostasis, LR/Paneth cells appear to play a supportive role for Lgr5(+) stem cells as previously shown, upon tissue injury they switch to a proliferating state and in the process activate Bmi1 expression while silencing Paneth-specific genes. Hence, they are likely to contribute to the regenerative process following tissue insults such as chronic inflammation. |
| format | Article |
| id | doaj-art-e67fd3c67cb14cf093871d4815ec7e29 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e67fd3c67cb14cf093871d4815ec7e292025-08-20T03:25:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3896510.1371/journal.pone.0038965Paneth cells in intestinal homeostasis and tissue injury.Sabrina RothPatrick FrankenAndrea SacchettiAndreas KremerKurt AndersonOwen SansomRiccardo FoddeAdult stem cell niches are often co-inhabited by cycling and quiescent stem cells. In the intestine, lineage tracing has identified Lgr5(+) cells as frequently cycling stem cells, whereas Bmi1(+), mTert(+), Hopx(+) and Lrig1(+) cells appear to be more quiescent. Here, we have applied a non-mutagenic and cell cycle independent approach to isolate and characterize small intestinal label-retaining cells (LRCs) persisting in the lower third of the crypt of Lieberkühn for up to 100 days. LRCs do not express markers of proliferation and of enterocyte, goblet or enteroendocrine differentiation, but are positive for Paneth cell markers. While during homeostasis, LR/Paneth cells appear to play a supportive role for Lgr5(+) stem cells as previously shown, upon tissue injury they switch to a proliferating state and in the process activate Bmi1 expression while silencing Paneth-specific genes. Hence, they are likely to contribute to the regenerative process following tissue insults such as chronic inflammation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038965&type=printable |
| spellingShingle | Sabrina Roth Patrick Franken Andrea Sacchetti Andreas Kremer Kurt Anderson Owen Sansom Riccardo Fodde Paneth cells in intestinal homeostasis and tissue injury. PLoS ONE |
| title | Paneth cells in intestinal homeostasis and tissue injury. |
| title_full | Paneth cells in intestinal homeostasis and tissue injury. |
| title_fullStr | Paneth cells in intestinal homeostasis and tissue injury. |
| title_full_unstemmed | Paneth cells in intestinal homeostasis and tissue injury. |
| title_short | Paneth cells in intestinal homeostasis and tissue injury. |
| title_sort | paneth cells in intestinal homeostasis and tissue injury |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038965&type=printable |
| work_keys_str_mv | AT sabrinaroth panethcellsinintestinalhomeostasisandtissueinjury AT patrickfranken panethcellsinintestinalhomeostasisandtissueinjury AT andreasacchetti panethcellsinintestinalhomeostasisandtissueinjury AT andreaskremer panethcellsinintestinalhomeostasisandtissueinjury AT kurtanderson panethcellsinintestinalhomeostasisandtissueinjury AT owensansom panethcellsinintestinalhomeostasisandtissueinjury AT riccardofodde panethcellsinintestinalhomeostasisandtissueinjury |