Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella
Abstract Trichinellosis is a zoonotic, foodborne parasitic infection causing muscle damage. This study investigated the potential therapeutic effects of the commercially available probiotic treatment Linex, both alone and in combination with Albendazole (ALB), on the intestinal and muscular stages o...
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BMC
2024-11-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-024-10202-9 |
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| author | Shimaa Attia Atta Zeinab H. Fahmy Eman A.H. Selim Tarek Aboushousha Reham Refaat Mostafa |
| author_facet | Shimaa Attia Atta Zeinab H. Fahmy Eman A.H. Selim Tarek Aboushousha Reham Refaat Mostafa |
| author_sort | Shimaa Attia Atta |
| collection | DOAJ |
| description | Abstract Trichinellosis is a zoonotic, foodborne parasitic infection causing muscle damage. This study investigated the potential therapeutic effects of the commercially available probiotic treatment Linex, both alone and in combination with Albendazole (ALB), on the intestinal and muscular stages of Trichinella spiralis infection in mice, assessing outcomes through parasitological, immunological, and histopathological measures. This study is the first to demonstrate the synergistic effect of combining the commercially available probiotic Linex with Albendazole for trichinellosis treatment. By enhancing both parasitological and immunological outcomes, this combined therapy not only significantly reduces parasite burden but also modulates the immune response, shifting it toward a protective Th1 profile. In parasitological terms, the highest adult and larval count reduction was observed in combined Linex and Albendazole treatment (100%, 97.7%) respectively. Lesser percentage of reduction were recorded in Linex alone therapy (43.2%, 88.4%) respectively. Histopathologically there was amelioration of the inflammatory cellular infiltration in all treated groups with best results in combined Linex and Albendazole treatment. Immunologically, serum IFN-γ levels increased significantly in all treated groups with highest levels in combined Linex and Albendazole treatment, while IL-4 and IL-13 level decreased significantly in all treated groups with best results observed in Linex alone treatment. To conclude; combined Linex and Albendazole treatment of mice infected with T. spirals could ameliorate the infection and improve the immune response. |
| format | Article |
| id | doaj-art-e67d2c1f07074e69b7adaff6170701da |
| institution | OA Journals |
| issn | 1471-2334 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Infectious Diseases |
| spelling | doaj-art-e67d2c1f07074e69b7adaff6170701da2025-08-20T02:08:19ZengBMCBMC Infectious Diseases1471-23342024-11-0124111210.1186/s12879-024-10202-9Effect of linex treatment on IFN-γ and IL-4 in mice infected with TrichinellaShimaa Attia Atta0Zeinab H. Fahmy1Eman A.H. Selim2Tarek Aboushousha3Reham Refaat Mostafa4Department of Immunology, Theodor Bilharz Research InstituteDepartment of Parasitology, Theodor Bilharz Research InstituteDepartment of Parasitology, Theodor Bilharz Research InstituteDepartment of Pathology, Theodor Bilharz Research InstituteDepartments of Medical Parasitology Faculty of Medicine, Cairo UniversityAbstract Trichinellosis is a zoonotic, foodborne parasitic infection causing muscle damage. This study investigated the potential therapeutic effects of the commercially available probiotic treatment Linex, both alone and in combination with Albendazole (ALB), on the intestinal and muscular stages of Trichinella spiralis infection in mice, assessing outcomes through parasitological, immunological, and histopathological measures. This study is the first to demonstrate the synergistic effect of combining the commercially available probiotic Linex with Albendazole for trichinellosis treatment. By enhancing both parasitological and immunological outcomes, this combined therapy not only significantly reduces parasite burden but also modulates the immune response, shifting it toward a protective Th1 profile. In parasitological terms, the highest adult and larval count reduction was observed in combined Linex and Albendazole treatment (100%, 97.7%) respectively. Lesser percentage of reduction were recorded in Linex alone therapy (43.2%, 88.4%) respectively. Histopathologically there was amelioration of the inflammatory cellular infiltration in all treated groups with best results in combined Linex and Albendazole treatment. Immunologically, serum IFN-γ levels increased significantly in all treated groups with highest levels in combined Linex and Albendazole treatment, while IL-4 and IL-13 level decreased significantly in all treated groups with best results observed in Linex alone treatment. To conclude; combined Linex and Albendazole treatment of mice infected with T. spirals could ameliorate the infection and improve the immune response.https://doi.org/10.1186/s12879-024-10202-9TrichinellosisProbioticLinexIFN-γIL-4IL-13 |
| spellingShingle | Shimaa Attia Atta Zeinab H. Fahmy Eman A.H. Selim Tarek Aboushousha Reham Refaat Mostafa Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella BMC Infectious Diseases Trichinellosis Probiotic Linex IFN-γ IL-4 IL-13 |
| title | Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella |
| title_full | Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella |
| title_fullStr | Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella |
| title_full_unstemmed | Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella |
| title_short | Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella |
| title_sort | effect of linex treatment on ifn γ and il 4 in mice infected with trichinella |
| topic | Trichinellosis Probiotic Linex IFN-γ IL-4 IL-13 |
| url | https://doi.org/10.1186/s12879-024-10202-9 |
| work_keys_str_mv | AT shimaaattiaatta effectoflinextreatmentonifngandil4inmiceinfectedwithtrichinella AT zeinabhfahmy effectoflinextreatmentonifngandil4inmiceinfectedwithtrichinella AT emanahselim effectoflinextreatmentonifngandil4inmiceinfectedwithtrichinella AT tarekaboushousha effectoflinextreatmentonifngandil4inmiceinfectedwithtrichinella AT rehamrefaatmostafa effectoflinextreatmentonifngandil4inmiceinfectedwithtrichinella |