Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker
Abstract EGFLAM is a versatile protein widely expressed in multiple human tissues, yet its function in pan-cancer is not fully understood. To investigate the molecular function of EGFLAM, a comprehensive pan-cancer analysis was carried out using several public databases and bioinformatics tools. Ult...
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BMC
2025-07-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-025-14519-9 |
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| author | Jian Yang Wenqian Xu Shengyu Wang Zhu Yu Yue Feng Da Li Junqiang Chen |
| author_facet | Jian Yang Wenqian Xu Shengyu Wang Zhu Yu Yue Feng Da Li Junqiang Chen |
| author_sort | Jian Yang |
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| description | Abstract EGFLAM is a versatile protein widely expressed in multiple human tissues, yet its function in pan-cancer is not fully understood. To investigate the molecular function of EGFLAM, a comprehensive pan-cancer analysis was carried out using several public databases and bioinformatics tools. Ultimately, confirmatory experiments were conducted in vitro with gastric cancer cell lines. The results showed that EGFLAM expression was elevated in multiple cancer types, especially gastric cancer. It also demonstrated prognostic predictive value in several cancer types, suggesting its potential as a prognostic biomarker. The dysregulation of EGFLAM expression observed in certain cancers might be attributed to factors such as promoter methylation changes, mRNA methylation modifications, and genetic alterations of the EGFLAM locus. EGFLAM expression was associated with Immune cell infiltration, immune checkpoints, tumor mutation burden (TMB), and microsatellite instability (MSI). Single-cell analysis of gastric cancer showed that EGFLAM was predominantly expressed in fibroblast populations. Functional enrichment analyses (GO/KEGG) demonstrated EGFLAM involvement in extracellular matrix receptor interactions and PI3K-AKT signaling pathway. RT‒qPCR confirmed EGFLAM upregulation in gastric cancer specimens compared to normal controls. In vitro functional assays revealed that EGFLAM knockdown significantly suppressed gastric cancer cell proliferation, migration, and invasion while inducing apoptosis. Collectively, our multi-omics analyses delineated EGFLAM's oncogenic functions across cancer types, positioning it as both a promising prognostic biomarker and a potential therapeutic target for gastric cancer. |
| format | Article |
| id | doaj-art-e67c20e8aa49420f911dcae276c16720 |
| institution | Kabale University |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-e67c20e8aa49420f911dcae276c167202025-08-20T03:45:30ZengBMCBMC Cancer1471-24072025-07-0125112410.1186/s12885-025-14519-9Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarkerJian Yang0Wenqian Xu1Shengyu Wang2Zhu Yu3Yue Feng4Da Li5Junqiang Chen6Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical UniversityGuangxi Key Laboratory of Enhanced Recovery After Surgery for Gastrointestinal CancerDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical UniversityAbstract EGFLAM is a versatile protein widely expressed in multiple human tissues, yet its function in pan-cancer is not fully understood. To investigate the molecular function of EGFLAM, a comprehensive pan-cancer analysis was carried out using several public databases and bioinformatics tools. Ultimately, confirmatory experiments were conducted in vitro with gastric cancer cell lines. The results showed that EGFLAM expression was elevated in multiple cancer types, especially gastric cancer. It also demonstrated prognostic predictive value in several cancer types, suggesting its potential as a prognostic biomarker. The dysregulation of EGFLAM expression observed in certain cancers might be attributed to factors such as promoter methylation changes, mRNA methylation modifications, and genetic alterations of the EGFLAM locus. EGFLAM expression was associated with Immune cell infiltration, immune checkpoints, tumor mutation burden (TMB), and microsatellite instability (MSI). Single-cell analysis of gastric cancer showed that EGFLAM was predominantly expressed in fibroblast populations. Functional enrichment analyses (GO/KEGG) demonstrated EGFLAM involvement in extracellular matrix receptor interactions and PI3K-AKT signaling pathway. RT‒qPCR confirmed EGFLAM upregulation in gastric cancer specimens compared to normal controls. In vitro functional assays revealed that EGFLAM knockdown significantly suppressed gastric cancer cell proliferation, migration, and invasion while inducing apoptosis. Collectively, our multi-omics analyses delineated EGFLAM's oncogenic functions across cancer types, positioning it as both a promising prognostic biomarker and a potential therapeutic target for gastric cancer.https://doi.org/10.1186/s12885-025-14519-9EGFLAMPan-cancerPrognosisImmune infiltrationGastric cancerBiomarker |
| spellingShingle | Jian Yang Wenqian Xu Shengyu Wang Zhu Yu Yue Feng Da Li Junqiang Chen Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker BMC Cancer EGFLAM Pan-cancer Prognosis Immune infiltration Gastric cancer Biomarker |
| title | Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker |
| title_full | Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker |
| title_fullStr | Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker |
| title_full_unstemmed | Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker |
| title_short | Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker |
| title_sort | comprehensive multi omics pan cancer analysis revealed egflam as a potential prognostic and immune infiltration associated biomarker |
| topic | EGFLAM Pan-cancer Prognosis Immune infiltration Gastric cancer Biomarker |
| url | https://doi.org/10.1186/s12885-025-14519-9 |
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