Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings

The world's growing population needs food, and aquaculture; a fast growing food producing sector, provides it. Fish meal (FM) is a major feed source in the aquaculture industry, but its low profitability and scarcity have prompted a quest for high-protein substitutes. Because silkworm pupa mea...

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Main Authors: Fatima Yasin, Muhammad Mudassar Shahzad, Syed Makhdoom Hussain, Syed Zakir Hussain Shah, Zawar Hussain, Waseem Abbas
Format: Article
Language:English
Published: BdFISH 2025-06-01
Series:Journal of Fisheries
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Online Access:https://journal.bdfish.org/index.php/fisheries/article/view/995
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author Fatima Yasin
Muhammad Mudassar Shahzad
Syed Makhdoom Hussain
Syed Zakir Hussain Shah
Zawar Hussain
Waseem Abbas
author_facet Fatima Yasin
Muhammad Mudassar Shahzad
Syed Makhdoom Hussain
Syed Zakir Hussain Shah
Zawar Hussain
Waseem Abbas
author_sort Fatima Yasin
collection DOAJ
description The world's growing population needs food, and aquaculture; a fast growing food producing sector, provides it. Fish meal (FM) is a major feed source in the aquaculture industry, but its low profitability and scarcity have prompted a quest for high-protein substitutes. Because silkworm pupa meal is inexpensive and has a high protein (50–60%) concentration, it was utilized as a substitute for fish feed. The goal of the current study was to determine the ideal proportion of silkworm pupa meal (SPM) to substitute for FM while creating an affordable feed for Cirrhinus mrigala fingerlings (average total length: 6 cm; average weight: 8 g). The fingerlings were fed six test diets (0, 20, 40, 60, 80, and 100%) at a rate of 4% of their live wet body weight twice a day. Diets contained chromium oxide as an inert marker. The feeding trial lasted for ninety days. The fingerlings' weight was measured every 14 days to calculate growth metrics (weight gain percentage, FCR, and SGR). Following the conclusion of the trial, intestinal histology, digestive enzyme function, and body indices were examined. Highest growth rate (WG%: 333%, SGR: 1.64, FCR: 1.26, CF: 1.50) and best body indices (hepatosomatic index: 1.95, viscerosomatic index: 6.43) values were observed at 40% replacement of FM. Intestinal histological analysis and digestive enzyme activity demonstrated that SPM can be readily substituted up to 40% without affecting the general performance of C. mrigala fingerlings and aid in creating an economical and environmentally friendly diet.
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institution Kabale University
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language English
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spelling doaj-art-e6752a829d7949d8a792bdf18e19f39e2025-08-20T03:31:23ZengBdFISHJournal of Fisheries2311-729X2311-31112025-06-0113210.17017/j.fish.995Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlingsFatima Yasin0Muhammad Mudassar Shahzad1Syed Makhdoom Hussain2Syed Zakir Hussain Shah3Zawar Hussain4Waseem Abbas5Department of Zoology, Division of Science and Technology, University of Education, Township, Lahore, PakistanDepartment of Zoology, Division of Science and Technology, University of Education, Township, Lahore, PakistanFish Nutrition Lab, Department of Zoology, Government College University Faisalabad, Faisalabad, PakistanDepartment of Zoology, University of Gujrat, Gujrat, PakistanDepartment of Zoology, Division of Science and Technology, University of Education, Township, Lahore, PakistanDepartment of Zoology, Division of Science and Technology, University of Education, Township, Lahore, Pakistan The world's growing population needs food, and aquaculture; a fast growing food producing sector, provides it. Fish meal (FM) is a major feed source in the aquaculture industry, but its low profitability and scarcity have prompted a quest for high-protein substitutes. Because silkworm pupa meal is inexpensive and has a high protein (50–60%) concentration, it was utilized as a substitute for fish feed. The goal of the current study was to determine the ideal proportion of silkworm pupa meal (SPM) to substitute for FM while creating an affordable feed for Cirrhinus mrigala fingerlings (average total length: 6 cm; average weight: 8 g). The fingerlings were fed six test diets (0, 20, 40, 60, 80, and 100%) at a rate of 4% of their live wet body weight twice a day. Diets contained chromium oxide as an inert marker. The feeding trial lasted for ninety days. The fingerlings' weight was measured every 14 days to calculate growth metrics (weight gain percentage, FCR, and SGR). Following the conclusion of the trial, intestinal histology, digestive enzyme function, and body indices were examined. Highest growth rate (WG%: 333%, SGR: 1.64, FCR: 1.26, CF: 1.50) and best body indices (hepatosomatic index: 1.95, viscerosomatic index: 6.43) values were observed at 40% replacement of FM. Intestinal histological analysis and digestive enzyme activity demonstrated that SPM can be readily substituted up to 40% without affecting the general performance of C. mrigala fingerlings and aid in creating an economical and environmentally friendly diet. https://journal.bdfish.org/index.php/fisheries/article/view/995body indicesCirrhinus mrigaladigestive enzymesfishmealhistopathologysilkworm pupa
spellingShingle Fatima Yasin
Muhammad Mudassar Shahzad
Syed Makhdoom Hussain
Syed Zakir Hussain Shah
Zawar Hussain
Waseem Abbas
Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings
Journal of Fisheries
body indices
Cirrhinus mrigala
digestive enzymes
fishmeal
histopathology
silkworm pupa
title Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings
title_full Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings
title_fullStr Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings
title_full_unstemmed Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings
title_short Evaluation of silkworm pupa meal on growth, body indices, digestive enzymes and histopathology of Cirrhinus mrigala fingerlings
title_sort evaluation of silkworm pupa meal on growth body indices digestive enzymes and histopathology of cirrhinus mrigala fingerlings
topic body indices
Cirrhinus mrigala
digestive enzymes
fishmeal
histopathology
silkworm pupa
url https://journal.bdfish.org/index.php/fisheries/article/view/995
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AT syedmakhdoomhussain evaluationofsilkwormpupamealongrowthbodyindicesdigestiveenzymesandhistopathologyofcirrhinusmrigalafingerlings
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