Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health
Antimicrobial resistance is a growing global threat, with surveillance providing essential information to control its spread and support rational treatment strategies. <i>Klebsiella pneumoniae</i>, a member of the Gram-negative Enterobacteriaceae family, frequently develops resistance me...
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MDPI AG
2025-04-01
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| Series: | Antibiotics |
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| author | Raquel Abreu Alice Matos Luís Capela Rita Jorge Joana F. Guerreiro Gonçalo Pereira Eva Cunha Lélia Chambel Luis Tavares Filip Boyen Manuela Oliveira |
| author_facet | Raquel Abreu Alice Matos Luís Capela Rita Jorge Joana F. Guerreiro Gonçalo Pereira Eva Cunha Lélia Chambel Luis Tavares Filip Boyen Manuela Oliveira |
| author_sort | Raquel Abreu |
| collection | DOAJ |
| description | Antimicrobial resistance is a growing global threat, with surveillance providing essential information to control its spread and support rational treatment strategies. <i>Klebsiella pneumoniae</i>, a member of the Gram-negative Enterobacteriaceae family, frequently develops resistance mechanisms. This study analyzed 195 rectal swabs from companion and stray dogs in Santiago and São Nicolau (Cape Verde) and São Tomé and Príncipe, sampled during a neutering and deworming campaign conducted by Veterinary Without Borders Portugal, to detect extended-spectrum β-lactamase (ESBL)-producing bacteria. Samples were enriched and then cultured on ChromID<sup>®</sup> ESBL agar, and resulting isolates were identified via MALDI-TOF MS. A total of 35 <i>K. pneumoniae</i> isolates were identified, of which 32 were confirmed as ESBL producers. Antimicrobial susceptibility testing showed 100% resistance to aztreonam, cefotaxime, cefpodoxime, and ceftaroline, and high resistance to cefepime (93.8%), ciprofloxacin (93.8%), and trimethoprim/sulfamethoxazole (90.6%). All isolates were considered multidrug-resistant but remained susceptible to cefoxitin, imipenem, and meropenem. The genes <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>SHV</sub>, and <i>bla</i><sub>TEM</sub> were present in 96.9%, 65.6%, and 56.3% of the isolates, respectively. DNA fingerprinting revealed seven clusters, suggesting genetic diversity and strain dissemination across locations. These findings highlight the role of dogs as vectors for antimicrobial resistance dissemination, underscoring the need for continuous surveillance in both veterinary and human medicine. |
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| institution | OA Journals |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-e673214814144b239f02584193fdc3b12025-08-20T02:24:39ZengMDPI AGAntibiotics2079-63822025-04-0114440810.3390/antibiotics14040408Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public HealthRaquel Abreu0Alice Matos1Luís Capela2Rita Jorge3Joana F. Guerreiro4Gonçalo Pereira5Eva Cunha6Lélia Chambel7Luis Tavares8Filip Boyen9Manuela Oliveira10CIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalAssociação Veterinários Sem Fronteiras, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalAssociação Veterinários Sem Fronteiras, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalBioISI—BioSystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalFaculty of Veterinary Medicine, Department of Pathobiology, Pharmacology and Zoological Medicine, Ghent University, 9000 Merelbeke, BelgiumCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalAntimicrobial resistance is a growing global threat, with surveillance providing essential information to control its spread and support rational treatment strategies. <i>Klebsiella pneumoniae</i>, a member of the Gram-negative Enterobacteriaceae family, frequently develops resistance mechanisms. This study analyzed 195 rectal swabs from companion and stray dogs in Santiago and São Nicolau (Cape Verde) and São Tomé and Príncipe, sampled during a neutering and deworming campaign conducted by Veterinary Without Borders Portugal, to detect extended-spectrum β-lactamase (ESBL)-producing bacteria. Samples were enriched and then cultured on ChromID<sup>®</sup> ESBL agar, and resulting isolates were identified via MALDI-TOF MS. A total of 35 <i>K. pneumoniae</i> isolates were identified, of which 32 were confirmed as ESBL producers. Antimicrobial susceptibility testing showed 100% resistance to aztreonam, cefotaxime, cefpodoxime, and ceftaroline, and high resistance to cefepime (93.8%), ciprofloxacin (93.8%), and trimethoprim/sulfamethoxazole (90.6%). All isolates were considered multidrug-resistant but remained susceptible to cefoxitin, imipenem, and meropenem. The genes <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>SHV</sub>, and <i>bla</i><sub>TEM</sub> were present in 96.9%, 65.6%, and 56.3% of the isolates, respectively. DNA fingerprinting revealed seven clusters, suggesting genetic diversity and strain dissemination across locations. These findings highlight the role of dogs as vectors for antimicrobial resistance dissemination, underscoring the need for continuous surveillance in both veterinary and human medicine.https://www.mdpi.com/2079-6382/14/4/408dog<i>Klebsiella pneumoniae</i>antimicrobial resistanceESBLmultidrug resistanceCape Verde |
| spellingShingle | Raquel Abreu Alice Matos Luís Capela Rita Jorge Joana F. Guerreiro Gonçalo Pereira Eva Cunha Lélia Chambel Luis Tavares Filip Boyen Manuela Oliveira Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health Antibiotics dog <i>Klebsiella pneumoniae</i> antimicrobial resistance ESBL multidrug resistance Cape Verde |
| title | Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health |
| title_full | Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health |
| title_fullStr | Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health |
| title_full_unstemmed | Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health |
| title_short | Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> in Dogs from Cape Verde and São Tomé and Príncipe: Implications for Public Health |
| title_sort | extended spectrum β lactamase producing i klebsiella pneumoniae i in dogs from cape verde and sao tome and principe implications for public health |
| topic | dog <i>Klebsiella pneumoniae</i> antimicrobial resistance ESBL multidrug resistance Cape Verde |
| url | https://www.mdpi.com/2079-6382/14/4/408 |
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