Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics

Members of the genus Hypoxylon (Ascomycota) are pleomorphic fungi mostly forming conspicuous teleomorphs, consisting of perithecia embedded into stromal tissue, and their morphology has traditionally served for species delineation. However, analysis in tandem with other phenotypic characters, such a...

Full description

Saved in:
Bibliographic Details
Main Authors: Marjorie Cedeño-Sanchez, Christopher Lambert, Luis C. Mejia, Sherif S. Ebada, Marc Stadler
Format: Article
Language:English
Published: Taylor & Francis Group 2025-01-01
Series:Mycology
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/21501203.2024.2378071
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849762960181821440
author Marjorie Cedeño-Sanchez
Christopher Lambert
Luis C. Mejia
Sherif S. Ebada
Marc Stadler
author_facet Marjorie Cedeño-Sanchez
Christopher Lambert
Luis C. Mejia
Sherif S. Ebada
Marc Stadler
author_sort Marjorie Cedeño-Sanchez
collection DOAJ
description Members of the genus Hypoxylon (Ascomycota) are pleomorphic fungi mostly forming conspicuous teleomorphs, consisting of perithecia embedded into stromal tissue, and their morphology has traditionally served for species delineation. However, analysis in tandem with other phenotypic characters, such as chemical and genetic traits, proved to be a more stable predictor of interspecies and intergeneric relationships. During 2014 and 2015, a set of species identified as Hypoxylon were described from the Neotropics, exclusively relying on morphological traits. The secondary metabolite profiles of their stromata were analysed by HPLC/DAD-ESI-MS, corroborating their classification within Xylariales. Additionally, molecular data for ex-type strains of H. dussii and H. sofaiense were incorporated into an inferred molecular phylogeny of the Hypoxylaceae and allies. Furthermore, a freshly collected specimen from North Carolina was selected as epitype of Sphaeria perforata Schweinitz (syn. Hypoxylon perforatum), as its morphological/chemotaxonomic characters matched those of the holotype. Our findings demonstrate that the secondary metabolism of Hypoxylon closely correlates with both morphological features and molecular data, serving as a complement for species identification.
format Article
id doaj-art-e65f52b619344e71aa33451ea59022d5
institution DOAJ
issn 2150-1203
2150-1211
language English
publishDate 2025-01-01
publisher Taylor & Francis Group
record_format Article
series Mycology
spelling doaj-art-e65f52b619344e71aa33451ea59022d52025-08-20T03:05:34ZengTaylor & Francis GroupMycology2150-12032150-12112025-01-0116125026510.1080/21501203.2024.2378071Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the NeotropicsMarjorie Cedeño-Sanchez0Christopher Lambert1Luis C. Mejia2Sherif S. Ebada3Marc Stadler4Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research, Braunschweig, GermanyDepartment of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research, Braunschweig, GermanyCentro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT–AIP), Panamá, Republic of PanamaDepartment of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research, Braunschweig, GermanyDepartment of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research, Braunschweig, GermanyMembers of the genus Hypoxylon (Ascomycota) are pleomorphic fungi mostly forming conspicuous teleomorphs, consisting of perithecia embedded into stromal tissue, and their morphology has traditionally served for species delineation. However, analysis in tandem with other phenotypic characters, such as chemical and genetic traits, proved to be a more stable predictor of interspecies and intergeneric relationships. During 2014 and 2015, a set of species identified as Hypoxylon were described from the Neotropics, exclusively relying on morphological traits. The secondary metabolite profiles of their stromata were analysed by HPLC/DAD-ESI-MS, corroborating their classification within Xylariales. Additionally, molecular data for ex-type strains of H. dussii and H. sofaiense were incorporated into an inferred molecular phylogeny of the Hypoxylaceae and allies. Furthermore, a freshly collected specimen from North Carolina was selected as epitype of Sphaeria perforata Schweinitz (syn. Hypoxylon perforatum), as its morphological/chemotaxonomic characters matched those of the holotype. Our findings demonstrate that the secondary metabolism of Hypoxylon closely correlates with both morphological features and molecular data, serving as a complement for species identification.https://www.tandfonline.com/doi/10.1080/21501203.2024.2378071Epitypephylogenystromatal pigmentsXylariales
spellingShingle Marjorie Cedeño-Sanchez
Christopher Lambert
Luis C. Mejia
Sherif S. Ebada
Marc Stadler
Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics
Mycology
Epitype
phylogeny
stromatal pigments
Xylariales
title Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics
title_full Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics
title_fullStr Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics
title_full_unstemmed Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics
title_short Chemotaxonomic and molecular phylogenetic studies of selected Hypoxylon species from the Neotropics
title_sort chemotaxonomic and molecular phylogenetic studies of selected hypoxylon species from the neotropics
topic Epitype
phylogeny
stromatal pigments
Xylariales
url https://www.tandfonline.com/doi/10.1080/21501203.2024.2378071
work_keys_str_mv AT marjoriecedenosanchez chemotaxonomicandmolecularphylogeneticstudiesofselectedhypoxylonspeciesfromtheneotropics
AT christopherlambert chemotaxonomicandmolecularphylogeneticstudiesofselectedhypoxylonspeciesfromtheneotropics
AT luiscmejia chemotaxonomicandmolecularphylogeneticstudiesofselectedhypoxylonspeciesfromtheneotropics
AT sherifsebada chemotaxonomicandmolecularphylogeneticstudiesofselectedhypoxylonspeciesfromtheneotropics
AT marcstadler chemotaxonomicandmolecularphylogeneticstudiesofselectedhypoxylonspeciesfromtheneotropics