Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease

Allogeneic cellular gene therapy through hematopoietic stem cell transplantation is the only radical cure for congenital hemoglobinopathies like thalassemia and sickle cell anemia. Persistent mixed hematopoietic chimerism (PMC) has been described in thalassemia and sickle cell anemia. Here, we descr...

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Main Authors: Marco Marziali, Antonella Isgrò, Pietro Sodani, Javid Gaziev, Daniela Fraboni, Katia Paciaroni, Cristiano Gallucci, Cecilia Alfieri, Andrea Roveda, Gioia De Angelis, Luisa Cardarelli, Michela Ribersani, Marco Andreani, Guido Lucarelli
Format: Article
Language:English
Published: PAGEPress Publications 2014-08-01
Series:Mediterranean Journal of Hematology and Infectious Diseases
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Online Access:http://www.mjhid.org/index.php/mjhid/article/view/2056
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author Marco Marziali
Antonella Isgrò
Pietro Sodani
Javid Gaziev
Daniela Fraboni
Katia Paciaroni
Cristiano Gallucci
Cecilia Alfieri
Andrea Roveda
Gioia De Angelis
Luisa Cardarelli
Michela Ribersani
Marco Andreani
Guido Lucarelli
author_facet Marco Marziali
Antonella Isgrò
Pietro Sodani
Javid Gaziev
Daniela Fraboni
Katia Paciaroni
Cristiano Gallucci
Cecilia Alfieri
Andrea Roveda
Gioia De Angelis
Luisa Cardarelli
Michela Ribersani
Marco Andreani
Guido Lucarelli
author_sort Marco Marziali
collection DOAJ
description Allogeneic cellular gene therapy through hematopoietic stem cell transplantation is the only radical cure for congenital hemoglobinopathies like thalassemia and sickle cell anemia. Persistent mixed hematopoietic chimerism (PMC) has been described in thalassemia and sickle cell anemia. Here, we describe the clinical course of a 6-year-old girl who had received bone marrow transplant for sickle cell anemia. After the transplant, the patient showed 36% donor hematopoietic stem cells in the bone marrow, whereas in the peripheral blood there was evidence of 80%  circulating donor red blood cells (RBC). The analysis of apoptosis at the Bone Marrow  level suggests that Fas might contribute to the cell death of host erythroid precursors. The increase in NK cells and the regulatory T cell population observed in this patient suggests that these cells might contribute to the condition of mixed chimerism.
format Article
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institution DOAJ
issn 2035-3006
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publishDate 2014-08-01
publisher PAGEPress Publications
record_format Article
series Mediterranean Journal of Hematology and Infectious Diseases
spelling doaj-art-e65dccd780f84903ad8c4c54612fc4222025-08-20T02:58:04ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062014-08-0161e2014066e201406610.4084/mjhid.2014.0661512Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell DiseaseMarco Marziali0Antonella Isgrò1Pietro Sodani2Javid Gaziev3Daniela Fraboni4Katia Paciaroni5Cristiano Gallucci6Cecilia Alfieri7Andrea Roveda8Gioia De Angelis9Luisa Cardarelli10Michela Ribersani11Marco Andreani12Guido Lucarelli13International Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyDepartment of Biopathology and Diagnostic Images, Polyclinic of Tor Vergata Foundation, Rome, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyLaboratory of Immunogenetics and Transplant Biology, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor VergataInternational Center for Transplantation in Thalassemia and Sickle Cell Anemia, Mediterranean Institute of Hematology, Policlinic of the University of Rome “Tor Vergata, ItalyAllogeneic cellular gene therapy through hematopoietic stem cell transplantation is the only radical cure for congenital hemoglobinopathies like thalassemia and sickle cell anemia. Persistent mixed hematopoietic chimerism (PMC) has been described in thalassemia and sickle cell anemia. Here, we describe the clinical course of a 6-year-old girl who had received bone marrow transplant for sickle cell anemia. After the transplant, the patient showed 36% donor hematopoietic stem cells in the bone marrow, whereas in the peripheral blood there was evidence of 80%  circulating donor red blood cells (RBC). The analysis of apoptosis at the Bone Marrow  level suggests that Fas might contribute to the cell death of host erythroid precursors. The increase in NK cells and the regulatory T cell population observed in this patient suggests that these cells might contribute to the condition of mixed chimerism.http://www.mjhid.org/index.php/mjhid/article/view/2056Sickle Cell DiseaseSplit ChimeirsmBone Marrow
spellingShingle Marco Marziali
Antonella Isgrò
Pietro Sodani
Javid Gaziev
Daniela Fraboni
Katia Paciaroni
Cristiano Gallucci
Cecilia Alfieri
Andrea Roveda
Gioia De Angelis
Luisa Cardarelli
Michela Ribersani
Marco Andreani
Guido Lucarelli
Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease
Mediterranean Journal of Hematology and Infectious Diseases
Sickle Cell Disease
Split Chimeirsm
Bone Marrow
title Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease
title_full Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease
title_fullStr Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease
title_full_unstemmed Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease
title_short Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease
title_sort peripheral red blood cell split chimerism as a consequence of intramedullary selective apoptosis of recipient red blood cells in a case of sickle cell disease
topic Sickle Cell Disease
Split Chimeirsm
Bone Marrow
url http://www.mjhid.org/index.php/mjhid/article/view/2056
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