Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda
Introduction: Staphylococcus epidermidis is often considered a non-pathogenic organism but it causes nosocomial infections. To distinguish invasive strains, comparative studies of patient and community isolates may offer some clues. We investigated the distribution of virulence determinants in pati...
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The Journal of Infection in Developing Countries
2011-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/2007 |
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| author | Moses S Okee Moses L Joloba Margaret Okello Florence Christine Najjuka Fred Ashaba Katabazi Freddie Bwanga Ann Nanteza David Patrick Kateete |
| author_facet | Moses S Okee Moses L Joloba Margaret Okello Florence Christine Najjuka Fred Ashaba Katabazi Freddie Bwanga Ann Nanteza David Patrick Kateete |
| author_sort | Moses S Okee |
| collection | DOAJ |
| description |
Introduction: Staphylococcus epidermidis is often considered a non-pathogenic organism but it causes nosocomial infections. To distinguish invasive strains, comparative studies of patient and community isolates may offer some clues. We investigated the distribution of virulence determinants in patient isolates from Uganda.
Methodology: S. epidermidis isolates were identified with the Staph API ID 32 kit. Antimicrobial susceptibility, biofilm formation and hemolysis were detected with standard procedures. Genes associated with virulence (aap, atlE, bhp, hla, hld, ica, IS256, sdrE, sea, tsst) and antimicrobial resistance (aac(6')-Ie-aph(2'')-Ia, aph(3')-IIIa, ant(4')-Ia, blaZ, mecA, vanA/vanB1) were detected by PCR.
Results: S. epidermidis grew in 30 (30/50, 60%) ICU samples and 20 (20/60, 33%) community samples (one isolate per sample per patient/person). All ICU isolates (30/30, 100%) were IS256 and hld positive, 22 (22/30, 73%) were biofilm/ica positive, 21 (21/30, 70%) were hemolytic on blood agar, nine (9/30, 30%) contained atlE gene, six (6/30, 20%) hla gene, five (5/30, 17%) aap gene, and three (3/30, 10%) bhp gene. A gene encoding an aminoglycoside-modifying enzyme, aph(3')-IIIa, was highly prevalent (28/30, 93%), while blaZ (2/30, 7%), mecA (3/30, 10%), vanA (3/30, 10%) and vanB1 (3/30, 10%) were less prevalent. Of the community isolates, one (1/20, 5%) was ica positive, two (2/20, 10%) formed biofilms, and three (3/20, 15%) possessed the atlE gene. bhp, aap, IS256, hld and antimicrobial resistance genes were not detected in community isolates.
Conclusions: S. epidermidis from ICU patients in Mulago Hospital is potentially virulent and could be a reservoir for antimicrobial resistant genes.
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| format | Article |
| id | doaj-art-e65a307731454ebca41b6662bcf080f6 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e65a307731454ebca41b6662bcf080f62025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802011-11-0160310.3855/jidc.2007Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, UgandaMoses S Okee0Moses L Joloba1Margaret Okello2Florence Christine Najjuka3Fred Ashaba Katabazi4Freddie Bwanga5Ann Nanteza6David Patrick Kateete7Makerere University College of Health Sciences, Kampala, UgandaMakerere University College of Health SciencesDepartment of Anesthesiology, Mulago National Referral HospitalMakerere University College of Health SciencesMakerere University College of Health SciencesMakerere University College of Health SciencesMakerere University School Veterinary MedicineMakerere University College of Health Sciences Introduction: Staphylococcus epidermidis is often considered a non-pathogenic organism but it causes nosocomial infections. To distinguish invasive strains, comparative studies of patient and community isolates may offer some clues. We investigated the distribution of virulence determinants in patient isolates from Uganda. Methodology: S. epidermidis isolates were identified with the Staph API ID 32 kit. Antimicrobial susceptibility, biofilm formation and hemolysis were detected with standard procedures. Genes associated with virulence (aap, atlE, bhp, hla, hld, ica, IS256, sdrE, sea, tsst) and antimicrobial resistance (aac(6')-Ie-aph(2'')-Ia, aph(3')-IIIa, ant(4')-Ia, blaZ, mecA, vanA/vanB1) were detected by PCR. Results: S. epidermidis grew in 30 (30/50, 60%) ICU samples and 20 (20/60, 33%) community samples (one isolate per sample per patient/person). All ICU isolates (30/30, 100%) were IS256 and hld positive, 22 (22/30, 73%) were biofilm/ica positive, 21 (21/30, 70%) were hemolytic on blood agar, nine (9/30, 30%) contained atlE gene, six (6/30, 20%) hla gene, five (5/30, 17%) aap gene, and three (3/30, 10%) bhp gene. A gene encoding an aminoglycoside-modifying enzyme, aph(3')-IIIa, was highly prevalent (28/30, 93%), while blaZ (2/30, 7%), mecA (3/30, 10%), vanA (3/30, 10%) and vanB1 (3/30, 10%) were less prevalent. Of the community isolates, one (1/20, 5%) was ica positive, two (2/20, 10%) formed biofilms, and three (3/20, 15%) possessed the atlE gene. bhp, aap, IS256, hld and antimicrobial resistance genes were not detected in community isolates. Conclusions: S. epidermidis from ICU patients in Mulago Hospital is potentially virulent and could be a reservoir for antimicrobial resistant genes. https://jidc.org/index.php/journal/article/view/2007Staphylococcus epidermidisvirulence determinantsantimicrobial resistance genesintensive care unitMulago HospitalUganda |
| spellingShingle | Moses S Okee Moses L Joloba Margaret Okello Florence Christine Najjuka Fred Ashaba Katabazi Freddie Bwanga Ann Nanteza David Patrick Kateete Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda Journal of Infection in Developing Countries Staphylococcus epidermidis virulence determinants antimicrobial resistance genes intensive care unit Mulago Hospital Uganda |
| title | Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda |
| title_full | Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda |
| title_fullStr | Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda |
| title_full_unstemmed | Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda |
| title_short | Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda |
| title_sort | prevalence of virulence determinants in staphylococcus epidermidis from icu patients in kampala uganda |
| topic | Staphylococcus epidermidis virulence determinants antimicrobial resistance genes intensive care unit Mulago Hospital Uganda |
| url | https://jidc.org/index.php/journal/article/view/2007 |
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