Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress
BackgroundSoil salinization poses a global threat to agriculture, necessitating strategies to enhance plant salt stress tolerance. Understanding the metabolic and transcriptomic responses of tobacco plants to salt stress is crucial for developing such strategies.ResultsThis study identified 238 up-r...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1614515/full |
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| author | Ge Bai Ge Bai Hui Zhang Hui Zhang Yong Li Da-Hai Yang Mingliang Fei Tao Pang Yaning Fu Ai-Guo Yang Zhen-Yu Wang Jinbao Gu He Xie |
| author_facet | Ge Bai Ge Bai Hui Zhang Hui Zhang Yong Li Da-Hai Yang Mingliang Fei Tao Pang Yaning Fu Ai-Guo Yang Zhen-Yu Wang Jinbao Gu He Xie |
| author_sort | Ge Bai |
| collection | DOAJ |
| description | BackgroundSoil salinization poses a global threat to agriculture, necessitating strategies to enhance plant salt stress tolerance. Understanding the metabolic and transcriptomic responses of tobacco plants to salt stress is crucial for developing such strategies.ResultsThis study identified 238 up-regulated and 122 down-regulated metabolites in tobacco plants under long-term salt stress. Initial stress stages activated galactose and sucrose metabolic pathways. Chlorophyll synthesis was impacted by decreased 5-aminolevulinic acid production, while proline accumulation helped mitigate cell damage. Metabolite-metabolite correlation analysis revealed significant correlations among metabolites, and enrichment analysis highlighted benzamides, amino acids, fatty acids, and monosaccharides. Transcriptome analysis identified 8,386 differentially expressed genes, with enriched pathways in hormone signaling, photosynthesis, and amino acid metabolism. Integrated analysis confirmed the involvement of sucrose pathway in the salt response, validated by qRT-PCR.ConclusionsThis study provides a comprehensive understanding of the regulatory networks in tobacco during salt stress. The findings lay the groundwork for future research on plant stress responses and the development of salt-tolerant tobacco cultivars. |
| format | Article |
| id | doaj-art-e656b117b6df48cc80abc67586a80bf7 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-e656b117b6df48cc80abc67586a80bf72025-08-20T03:31:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-06-011610.3389/fpls.2025.16145151614515Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stressGe Bai0Ge Bai1Hui Zhang2Hui Zhang3Yong Li4Da-Hai Yang5Mingliang Fei6Tao Pang7Yaning Fu8Ai-Guo Yang9Zhen-Yu Wang10Jinbao Gu11He Xie12Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Science, Kunming, Yunnan, ChinaApplication Department 1, Beijing Life Science Academy, Beijing, ChinaApplication Department 1, Beijing Life Science Academy, Beijing, ChinaNational Tobacco Gene Research Centre, Zhengzhou Tobacco Research Institute, Zhengzhou, Henan, ChinaKey Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Science, Kunming, Yunnan, ChinaKey Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Science, Kunming, Yunnan, ChinaKey Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Science, Kunming, Yunnan, ChinaKey Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Science, Kunming, Yunnan, ChinaApplication Department 1, Beijing Life Science Academy, Beijing, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou, Guangdong, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Science, Kunming, Yunnan, ChinaBackgroundSoil salinization poses a global threat to agriculture, necessitating strategies to enhance plant salt stress tolerance. Understanding the metabolic and transcriptomic responses of tobacco plants to salt stress is crucial for developing such strategies.ResultsThis study identified 238 up-regulated and 122 down-regulated metabolites in tobacco plants under long-term salt stress. Initial stress stages activated galactose and sucrose metabolic pathways. Chlorophyll synthesis was impacted by decreased 5-aminolevulinic acid production, while proline accumulation helped mitigate cell damage. Metabolite-metabolite correlation analysis revealed significant correlations among metabolites, and enrichment analysis highlighted benzamides, amino acids, fatty acids, and monosaccharides. Transcriptome analysis identified 8,386 differentially expressed genes, with enriched pathways in hormone signaling, photosynthesis, and amino acid metabolism. Integrated analysis confirmed the involvement of sucrose pathway in the salt response, validated by qRT-PCR.ConclusionsThis study provides a comprehensive understanding of the regulatory networks in tobacco during salt stress. The findings lay the groundwork for future research on plant stress responses and the development of salt-tolerant tobacco cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2025.1614515/fullsalt stressmetabolomicstranscriptomicstobacco plantsmolecular mechanismssalt tolerance |
| spellingShingle | Ge Bai Ge Bai Hui Zhang Hui Zhang Yong Li Da-Hai Yang Mingliang Fei Tao Pang Yaning Fu Ai-Guo Yang Zhen-Yu Wang Jinbao Gu He Xie Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress Frontiers in Plant Science salt stress metabolomics transcriptomics tobacco plants molecular mechanisms salt tolerance |
| title | Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress |
| title_full | Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress |
| title_fullStr | Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress |
| title_full_unstemmed | Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress |
| title_short | Integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco (Nicotiana tabacum) under salt stress |
| title_sort | integration of metabolic and transcriptomic analyses for revealing the galactose metabolism of tobacco nicotiana tabacum under salt stress |
| topic | salt stress metabolomics transcriptomics tobacco plants molecular mechanisms salt tolerance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1614515/full |
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