Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution

The bones of kedukang fish, a by-product of jambal roti production in Pangandaran, have the potential to be a source of collagen. Collagen extraction can be done chemically using acetic acid solution. The extraction process is declared effective if it produces optimal collagen yield. This study aims...

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Main Authors: Veronica Veronica, Junianto Junianto, Aulia Andhikawati, Emma Rochima
Format: Article
Language:English
Published: Litbang Pemas Universitas Islam Lamongan 2025-04-01
Series:Grouper
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Online Access:https://grouper.unisla.ac.id/index.php/grouper/article/view/319
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author Veronica Veronica
Junianto Junianto
Aulia Andhikawati
Emma Rochima
author_facet Veronica Veronica
Junianto Junianto
Aulia Andhikawati
Emma Rochima
author_sort Veronica Veronica
collection DOAJ
description The bones of kedukang fish, a by-product of jambal roti production in Pangandaran, have the potential to be a source of collagen. Collagen extraction can be done chemically using acetic acid solution. The extraction process is declared effective if it produces optimal collagen yield. This study aims to determine the best acetic acid concentration in producing optimal collagen yield. The research was conducted from December 2024 to February 2025 at the Tropical Marine Fisheries Laboratory and Central Laboratory of Padjadjaran University and the Center for Testing and Application of Fishery Product Quality (BP2MHP) Semarang. The method used was experimental method, namely the difference in acetic acid concentration in collagen extraction with three treatments (0.5 M; 0.75 M; and 1 M). Parameters observed included yield, physicochemical characteristics and functional groups of collagen from the best treatment. Based on the results, 1 M acetic acid concentration treatment was the best treatment with a yield value of 34.62% ± 1.31. The characteristics of collagen produced include moisture content of 9.507%, ash content of 2.60%, protein content of 64.91% and pH 6.94. FTIR results showed collagen identified Amide A at the absorption peak of 3404 cm-¹, Amide I at the absorption peak of 1698.40 cm-¹ and 1636.98 cm-¹, Amide II at the absorption peak of 1546.40 cm-¹ and Amide III at the absorption peak of 1230.36 cm-¹.
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spelling doaj-art-e655bf249e6e4fd79b72da0fe9b6f5c02025-08-20T02:38:23ZengLitbang Pemas Universitas Islam LamonganGrouper2086-84802716-27022025-04-01161249266https://doi.org/10.30736/grouper.v16i1.319Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid SolutionVeronica Veronica0Junianto Junianto1Aulia Andhikawati2Emma Rochima3Department of Tropical Marine Fisheries, Faculty of Fisheries and Marine Sciences, Universitas PadjadjaranDepartment of Tropical Marine Fisheries, Faculty of Fisheries and Marine Sciences, Universitas PadjadjaranDepartment of Tropical Marine Fisheries, Faculty of Fisheries and Marine Sciences, Universitas PadjadjaranDepartment of Tropical Marine Fisheries, Faculty of Fisheries and Marine Sciences, Universitas PadjadjaranThe bones of kedukang fish, a by-product of jambal roti production in Pangandaran, have the potential to be a source of collagen. Collagen extraction can be done chemically using acetic acid solution. The extraction process is declared effective if it produces optimal collagen yield. This study aims to determine the best acetic acid concentration in producing optimal collagen yield. The research was conducted from December 2024 to February 2025 at the Tropical Marine Fisheries Laboratory and Central Laboratory of Padjadjaran University and the Center for Testing and Application of Fishery Product Quality (BP2MHP) Semarang. The method used was experimental method, namely the difference in acetic acid concentration in collagen extraction with three treatments (0.5 M; 0.75 M; and 1 M). Parameters observed included yield, physicochemical characteristics and functional groups of collagen from the best treatment. Based on the results, 1 M acetic acid concentration treatment was the best treatment with a yield value of 34.62% ± 1.31. The characteristics of collagen produced include moisture content of 9.507%, ash content of 2.60%, protein content of 64.91% and pH 6.94. FTIR results showed collagen identified Amide A at the absorption peak of 3404 cm-¹, Amide I at the absorption peak of 1698.40 cm-¹ and 1636.98 cm-¹, Amide II at the absorption peak of 1546.40 cm-¹ and Amide III at the absorption peak of 1230.36 cm-¹.https://grouper.unisla.ac.id/index.php/grouper/article/view/319amidesfunctional groupsjambal rotiproksimatyield
spellingShingle Veronica Veronica
Junianto Junianto
Aulia Andhikawati
Emma Rochima
Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution
Grouper
amides
functional groups
jambal roti
proksimat
yield
title Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution
title_full Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution
title_fullStr Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution
title_full_unstemmed Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution
title_short Extraction of Collagen from Kedukang Fish Bone (Hexanematichthys sagor) at Various Concentrations of Acetic Acid Solution
title_sort extraction of collagen from kedukang fish bone hexanematichthys sagor at various concentrations of acetic acid solution
topic amides
functional groups
jambal roti
proksimat
yield
url https://grouper.unisla.ac.id/index.php/grouper/article/view/319
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AT juniantojunianto extractionofcollagenfromkedukangfishbonehexanematichthyssagoratvariousconcentrationsofaceticacidsolution
AT auliaandhikawati extractionofcollagenfromkedukangfishbonehexanematichthyssagoratvariousconcentrationsofaceticacidsolution
AT emmarochima extractionofcollagenfromkedukangfishbonehexanematichthyssagoratvariousconcentrationsofaceticacidsolution