Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology

A statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolated Saccharomyces cerevisiae Y-39. Response surface methodology RSM based on central composite face centered design CCFD was employed to statis...

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Main Authors: Nour Sh. El-Gendy, Hekmat R. Madian, Salem S. Abu Amr
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2013/815631
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author Nour Sh. El-Gendy
Hekmat R. Madian
Salem S. Abu Amr
author_facet Nour Sh. El-Gendy
Hekmat R. Madian
Salem S. Abu Amr
author_sort Nour Sh. El-Gendy
collection DOAJ
description A statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolated Saccharomyces cerevisiae Y-39. Response surface methodology RSM based on central composite face centered design CCFD was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial pH, incubation temperature, and molasses concentration on bioethanol yield. With the use of the developed quadratic model equation, a maximum ethanol production of 255 g/L was obtained in a batch fermentation process at optimum operating conditions of approximately 71 h, pH 5.6, 38°C, molasses concentration 18% wt.%, and 100 rpm.
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institution Kabale University
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publishDate 2013-01-01
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series International Journal of Microbiology
spelling doaj-art-e64ba433066542898a0b03610aa1de872025-02-03T05:44:37ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/815631815631Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface MethodologyNour Sh. El-Gendy0Hekmat R. Madian1Salem S. Abu Amr2Egyptian Petroleum Research Institute, Nasr City, Cairo 11727, EgyptEgyptian Petroleum Research Institute, Nasr City, Cairo 11727, EgyptSchool of Civil Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, MalaysiaA statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolated Saccharomyces cerevisiae Y-39. Response surface methodology RSM based on central composite face centered design CCFD was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial pH, incubation temperature, and molasses concentration on bioethanol yield. With the use of the developed quadratic model equation, a maximum ethanol production of 255 g/L was obtained in a batch fermentation process at optimum operating conditions of approximately 71 h, pH 5.6, 38°C, molasses concentration 18% wt.%, and 100 rpm.http://dx.doi.org/10.1155/2013/815631
spellingShingle Nour Sh. El-Gendy
Hekmat R. Madian
Salem S. Abu Amr
Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
International Journal of Microbiology
title Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
title_full Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
title_fullStr Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
title_full_unstemmed Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
title_short Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
title_sort design and optimization of a process for sugarcane molasses fermentation by saccharomyces cerevisiae using response surface methodology
url http://dx.doi.org/10.1155/2013/815631
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