Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss

This study investigates the therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss. It includes an in vitro phase to formulate an ethanolic extract of Algerian propolis (EEPA) ointment and evaluate its antimicrobial activity, and an in vivo phase to compare i...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdellatif Boudra, Rachid Merati, Berrani Abdelkader, Wassim Yezli, Helali Hadil, Imen Henni
Format: Article
Language:English
Published: Universidad del Zulia 2025-06-01
Series:Revista Científica
Subjects:
Online Access:https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43958
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850218694942130176
author Abdellatif Boudra
Rachid Merati
Berrani Abdelkader
Wassim Yezli
Helali Hadil
Imen Henni
author_facet Abdellatif Boudra
Rachid Merati
Berrani Abdelkader
Wassim Yezli
Helali Hadil
Imen Henni
author_sort Abdellatif Boudra
collection DOAJ
description This study investigates the therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss. It includes an in vitro phase to formulate an ethanolic extract of Algerian propolis (EEPA) ointment and evaluate its antimicrobial activity, and an in vivo phase to compare its effects with silver sulfadiazine cream and a control group in rabbit wound healing. In vitro, two propolis samples (P1 and P2) were tested against Staphylococcus aureus, showing inhibition zones of 9.5 ± 1.04 mm (P1) and 11.8 ± 0.65 mm (P2). In vivo, the propolis–treated group (PG) achieved complete wound closure (16 cm²) within 16–30 days (d), compared to 24–36 d for the silver sulfadiazine group (SDG), while the control group (CG) did not achieve full closure after 36 d. Healing scores were highest in PG (2.27 ± 0.59 to 2.80 ± 0.46) and dressing evaluation scores were also superior ± 51). Faster fur regrowth was observed in PG, enhancing wound aesthetics. Postoperative hypothermia affected all groups, with CG experiencing the greatest temperature drop (>2°C) and higher mortality. In conclusion, Algerian propolis, particularly P2, exhibited superior activity due to its higher polyphenol content, demonstrating its potential as a natural antimicrobial agent shows strong potential in wound management by enhancing antimicrobial protection, accelerating healing, and improving wound aesthetics. Further studies are needed to optimize its application and elucidate its mechanisms of action.
format Article
id doaj-art-e647c5cdd3a64dd99d6918fe531514d4
institution OA Journals
issn 0798-2259
2521-9715
language English
publishDate 2025-06-01
publisher Universidad del Zulia
record_format Article
series Revista Científica
spelling doaj-art-e647c5cdd3a64dd99d6918fe531514d42025-08-20T02:07:38ZengUniversidad del ZuliaRevista Científica0798-22592521-97152025-06-0135210.52973/rcfcv-e35658Therapeutic properties of Algerian propolis in skin wound healing with significant tissue lossAbdellatif Boudra0Rachid Merati 1Berrani Abdelkader2Wassim Yezli 3Helali Hadil4Imen Henni 5University of Tiaret, Laboratory of Hygiene and Animal Pathology. 14000, Tiaret, Algeria.University of Tiaret, Laboratory of Hygiene and Animal Pathology. 14000, Tiaret, Algeria.University of Tiaret, Faculty of Natural and Life Sciences. 14000, Algeria.University of Tiaret, Faculty of Natural and Life Sciences. 14000, Algeria.University of Tiaret, Faculty of Natural and Life Sciences. 14000, Algeria.University of Tiaret, Faculty of Natural and Life Sciences. 14000, Algeria. This study investigates the therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss. It includes an in vitro phase to formulate an ethanolic extract of Algerian propolis (EEPA) ointment and evaluate its antimicrobial activity, and an in vivo phase to compare its effects with silver sulfadiazine cream and a control group in rabbit wound healing. In vitro, two propolis samples (P1 and P2) were tested against Staphylococcus aureus, showing inhibition zones of 9.5 ± 1.04 mm (P1) and 11.8 ± 0.65 mm (P2). In vivo, the propolis–treated group (PG) achieved complete wound closure (16 cm²) within 16–30 days (d), compared to 24–36 d for the silver sulfadiazine group (SDG), while the control group (CG) did not achieve full closure after 36 d. Healing scores were highest in PG (2.27 ± 0.59 to 2.80 ± 0.46) and dressing evaluation scores were also superior ± 51). Faster fur regrowth was observed in PG, enhancing wound aesthetics. Postoperative hypothermia affected all groups, with CG experiencing the greatest temperature drop (>2°C) and higher mortality. In conclusion, Algerian propolis, particularly P2, exhibited superior activity due to its higher polyphenol content, demonstrating its potential as a natural antimicrobial agent shows strong potential in wound management by enhancing antimicrobial protection, accelerating healing, and improving wound aesthetics. Further studies are needed to optimize its application and elucidate its mechanisms of action. https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43958Propoliswound healingantimicrobial propertiesin vitro studyin vivo study
spellingShingle Abdellatif Boudra
Rachid Merati
Berrani Abdelkader
Wassim Yezli
Helali Hadil
Imen Henni
Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss
Revista Científica
Propolis
wound healing
antimicrobial properties
in vitro study
in vivo study
title Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss
title_full Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss
title_fullStr Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss
title_full_unstemmed Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss
title_short Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss
title_sort therapeutic properties of algerian propolis in skin wound healing with significant tissue loss
topic Propolis
wound healing
antimicrobial properties
in vitro study
in vivo study
url https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43958
work_keys_str_mv AT abdellatifboudra therapeuticpropertiesofalgerianpropolisinskinwoundhealingwithsignificanttissueloss
AT rachidmerati therapeuticpropertiesofalgerianpropolisinskinwoundhealingwithsignificanttissueloss
AT berraniabdelkader therapeuticpropertiesofalgerianpropolisinskinwoundhealingwithsignificanttissueloss
AT wassimyezli therapeuticpropertiesofalgerianpropolisinskinwoundhealingwithsignificanttissueloss
AT helalihadil therapeuticpropertiesofalgerianpropolisinskinwoundhealingwithsignificanttissueloss
AT imenhenni therapeuticpropertiesofalgerianpropolisinskinwoundhealingwithsignificanttissueloss