Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae

Trospectomycin, unlike aminoglycosidic aminocyclitols, is accumulated by a nonsaturable, energy-independent, diffusional process in Salmonella typhimurium, Escherichia coli and Haemophilus influenzae. A deep rough mutant of S typhimurium was more susceptible and accumulated the drug faster, and F po...

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Main Authors: S Wong, LE Bryan
Format: Article
Language:English
Published: Wiley 1990-01-01
Series:Canadian Journal of Infectious Diseases
Online Access:http://dx.doi.org/10.1155/1990/258273
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author S Wong
LE Bryan
author_facet S Wong
LE Bryan
author_sort S Wong
collection DOAJ
description Trospectomycin, unlike aminoglycosidic aminocyclitols, is accumulated by a nonsaturable, energy-independent, diffusional process in Salmonella typhimurium, Escherichia coli and Haemophilus influenzae. A deep rough mutant of S typhimurium was more susceptible and accumulated the drug faster, and F porin deficient mutants of E coli were more resistant than parental strains. Trospectomycin likely uses both porin and nonporin pathways to cross the outer membrane. An E coli strain effectively accumulated the drug anaerobically, explaining its anaerobic activity. An H influenzae strain accumulated trospectomycin at concentrations below those for which detectable uptake could be observed with E coli or S typhimurium strains, consistent with greater activity in Haemophilus species.
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issn 1180-2332
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series Canadian Journal of Infectious Diseases
spelling doaj-art-e6479a75194d43c6893778f937657f942025-08-20T02:21:03ZengWileyCanadian Journal of Infectious Diseases1180-23321990-01-0112515610.1155/1990/258273Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzaeS Wong0LE Bryan1Departments of Microbiology and Infectious Diseases, University of Calgary and Foothills Hospital, Calgary, Alberta, CanadaDepartments of Microbiology and Infectious Diseases, University of Calgary and Foothills Hospital, Calgary, Alberta, CanadaTrospectomycin, unlike aminoglycosidic aminocyclitols, is accumulated by a nonsaturable, energy-independent, diffusional process in Salmonella typhimurium, Escherichia coli and Haemophilus influenzae. A deep rough mutant of S typhimurium was more susceptible and accumulated the drug faster, and F porin deficient mutants of E coli were more resistant than parental strains. Trospectomycin likely uses both porin and nonporin pathways to cross the outer membrane. An E coli strain effectively accumulated the drug anaerobically, explaining its anaerobic activity. An H influenzae strain accumulated trospectomycin at concentrations below those for which detectable uptake could be observed with E coli or S typhimurium strains, consistent with greater activity in Haemophilus species.http://dx.doi.org/10.1155/1990/258273
spellingShingle S Wong
LE Bryan
Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae
Canadian Journal of Infectious Diseases
title Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae
title_full Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae
title_fullStr Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae
title_full_unstemmed Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae
title_short Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae
title_sort accumulation of trospectomycin by strains of salmonella typhimurium escherichia coli and haemophilus influenzae
url http://dx.doi.org/10.1155/1990/258273
work_keys_str_mv AT swong accumulationoftrospectomycinbystrainsofsalmonellatyphimuriumescherichiacoliandhaemophilusinfluenzae
AT lebryan accumulationoftrospectomycinbystrainsofsalmonellatyphimuriumescherichiacoliandhaemophilusinfluenzae