Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure
Objectives: Perchlorates, nitrates, and thiocyanates constitute environmental endocrine disruptors; however, health damage caused by absorption through the respiratory tract remains poorly studied. We investigated the effects of inhalation of these pollutants on thyroid function and structure and se...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132500003X |
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| author | Chenchen Wang Xiaohui Xu Jiangling Yuan Ran An Aierken Taxitiemuer Yan Zhang Tao Luo Jing Zhou Chang Wang Ling Zhang Shunhua Wu |
| author_facet | Chenchen Wang Xiaohui Xu Jiangling Yuan Ran An Aierken Taxitiemuer Yan Zhang Tao Luo Jing Zhou Chang Wang Ling Zhang Shunhua Wu |
| author_sort | Chenchen Wang |
| collection | DOAJ |
| description | Objectives: Perchlorates, nitrates, and thiocyanates constitute environmental endocrine disruptors; however, health damage caused by absorption through the respiratory tract remains poorly studied. We investigated the effects of inhalation of these pollutants on thyroid function and structure and serum metabolomics in pregnant rats. Methods: We established a Sprague-Dawley pregnant rat model exposed to perchlorate, nitrate, and thiocyanate at different gestational stages and compared maternal serum thyroid function levels, foetal development, thyroid morphology, and pathological changes between exposed and non-exposed groups at different concentrations. We used liquid chromatography-tandem mass spectrometry metabolomics methods to investigate corresponding changes in serum metabolites and metabolic biomarkers and identify metabolism-related pathways. Results: Free triiodothyronine levels in the medium-and high-concentration groups and free thyroxine levels in the low-, medium-, and high-concentration groups were significantly lower than those in the control group. Weight gain was significantly lower in the high-dose group than in the control group. Thyroid weight and organ coefficients were significantly higher in the medium-and high-dose groups at 15 and 19 days of pregnancy than in the control group. Thyroid epithelial cells demonstrated diffuse proliferation and lamination with increased atomisation concentrations, and thyroid cells tended to undergo apoptosis in the group with high thyroid epithelial cell concentration. Eight and 18 metabolic pathways were significantly affected in the GD15 and GD19 groups（GD15: The demise occurring 15 days into gestation,GD19: The demise occurring 15 days into gestation.）, respectively, post-exposure. The involved pathways included lipid, glucose, bile acid, choline, and gamma-aminobutyric acid metabolism and peroxisome proliferation-activated receptors. Conclusions: Mixed thiocyanate, nitrate, and perchlorate exposure can result in subchronic toxicity in rats, affecting thyroid function and structure. |
| format | Article |
| id | doaj-art-e644737b4b524ef9b3df6937aa163c1e |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e644737b4b524ef9b3df6937aa163c1e2025-01-23T05:26:02ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117667Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposureChenchen Wang0Xiaohui Xu1Jiangling Yuan2Ran An3Aierken Taxitiemuer4Yan Zhang5Tao Luo6Jing Zhou7Chang Wang8Ling Zhang9Shunhua Wu10School of Public Health, Xinjiang Medical University, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi, ChinaXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi, China; Corresponding authors.School of Public Health, Xinjiang Medical University, Urumqi, China; Corresponding authors.Objectives: Perchlorates, nitrates, and thiocyanates constitute environmental endocrine disruptors; however, health damage caused by absorption through the respiratory tract remains poorly studied. We investigated the effects of inhalation of these pollutants on thyroid function and structure and serum metabolomics in pregnant rats. Methods: We established a Sprague-Dawley pregnant rat model exposed to perchlorate, nitrate, and thiocyanate at different gestational stages and compared maternal serum thyroid function levels, foetal development, thyroid morphology, and pathological changes between exposed and non-exposed groups at different concentrations. We used liquid chromatography-tandem mass spectrometry metabolomics methods to investigate corresponding changes in serum metabolites and metabolic biomarkers and identify metabolism-related pathways. Results: Free triiodothyronine levels in the medium-and high-concentration groups and free thyroxine levels in the low-, medium-, and high-concentration groups were significantly lower than those in the control group. Weight gain was significantly lower in the high-dose group than in the control group. Thyroid weight and organ coefficients were significantly higher in the medium-and high-dose groups at 15 and 19 days of pregnancy than in the control group. Thyroid epithelial cells demonstrated diffuse proliferation and lamination with increased atomisation concentrations, and thyroid cells tended to undergo apoptosis in the group with high thyroid epithelial cell concentration. Eight and 18 metabolic pathways were significantly affected in the GD15 and GD19 groups（GD15: The demise occurring 15 days into gestation,GD19: The demise occurring 15 days into gestation.）, respectively, post-exposure. The involved pathways included lipid, glucose, bile acid, choline, and gamma-aminobutyric acid metabolism and peroxisome proliferation-activated receptors. Conclusions: Mixed thiocyanate, nitrate, and perchlorate exposure can result in subchronic toxicity in rats, affecting thyroid function and structure.http://www.sciencedirect.com/science/article/pii/S014765132500003XMaternal and child healthPerchlorate, thiocyanate, and nitrateThyroid hormonesSprague Dawley RatAir pollution |
| spellingShingle | Chenchen Wang Xiaohui Xu Jiangling Yuan Ran An Aierken Taxitiemuer Yan Zhang Tao Luo Jing Zhou Chang Wang Ling Zhang Shunhua Wu Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure Ecotoxicology and Environmental Safety Maternal and child health Perchlorate, thiocyanate, and nitrate Thyroid hormones Sprague Dawley Rat Air pollution |
| title | Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure |
| title_full | Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure |
| title_fullStr | Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure |
| title_full_unstemmed | Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure |
| title_short | Analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants: Combined perchlorate, thiocyanate, and nitrate exposure |
| title_sort | analysis of thyroid function and structure and serum metabolomics in pregnant rats exposed to airborne contaminants combined perchlorate thiocyanate and nitrate exposure |
| topic | Maternal and child health Perchlorate, thiocyanate, and nitrate Thyroid hormones Sprague Dawley Rat Air pollution |
| url | http://www.sciencedirect.com/science/article/pii/S014765132500003X |
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