Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia
Introduction: The widespread use of highly active antiretroviral therapy (HAART) and continuous reports of HIV-1 strains developing resistance to these drugs is rather alarming, as transmission of resistant viruses to newly infected persons is possible. This study aimed to determine HIV-1 subtypes a...
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The Journal of Infection in Developing Countries
2014-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/4095 |
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| author | Tengku Ahmad Akram Tengku Mohd Ariffin Suharni Mohamad Wan Nazirah Wan Yusuf Rafidah Hanim Shueb |
| author_facet | Tengku Ahmad Akram Tengku Mohd Ariffin Suharni Mohamad Wan Nazirah Wan Yusuf Rafidah Hanim Shueb |
| author_sort | Tengku Ahmad Akram Tengku Mohd Ariffin |
| collection | DOAJ |
| description | Introduction: The widespread use of highly active antiretroviral therapy (HAART) and continuous reports of HIV-1 strains developing resistance to these drugs is rather alarming, as transmission of resistant viruses to newly infected persons is possible. This study aimed to determine HIV-1 subtypes and the prevalence of primary mutations associated with antiretroviral (ARV) resistance among treatment-naive prisoners on the east coast of Malaysia.
Methodology: Viral RNA was extracted from plasma samples of 21 treatment-naive prisoners. Protease (PR) and reverse transcriptase (RT) regions were amplified and sequenced. Stanford HIV database algorithms were used for interpretation of resistance, and phylogenetic analysis was performed for subtype assignment.
Results: In the PR gene, no antiviral resistance-associated mutation was detected. For RT-associated mutations, K103N was the most prevalent in sequenced samples (14.3%). Genetic subtyping on the pol gene revealed that the majority of the prisoners were infected with subtype CRF33_01B (52.4%).
Conclusion: Continuous surveillance of newly infected individuals is required to help strategize the best antiviral treatment for these patients.
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| format | Article |
| id | doaj-art-e64345e8de7f4773a073ea3f8ed9efac |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e64345e8de7f4773a073ea3f8ed9efac2025-08-20T02:27:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-08-0180810.3855/jidc.4095Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, MalaysiaTengku Ahmad Akram Tengku Mohd Ariffin0Suharni Mohamad1Wan Nazirah Wan Yusuf2Rafidah Hanim Shueb3Universiti Sains Malaysia, Kubang Kerian, Kelantan, MalaysiaUniversiti Sains Malaysia, Kubang Kerian, Kelantan, MalaysiaUniversiti Sains Malaysia, Kubang Kerian, Kelantan, MalaysiaUniversiti Sains Malaysia, Kubang Kerian, Kelantan, MalaysiaIntroduction: The widespread use of highly active antiretroviral therapy (HAART) and continuous reports of HIV-1 strains developing resistance to these drugs is rather alarming, as transmission of resistant viruses to newly infected persons is possible. This study aimed to determine HIV-1 subtypes and the prevalence of primary mutations associated with antiretroviral (ARV) resistance among treatment-naive prisoners on the east coast of Malaysia. Methodology: Viral RNA was extracted from plasma samples of 21 treatment-naive prisoners. Protease (PR) and reverse transcriptase (RT) regions were amplified and sequenced. Stanford HIV database algorithms were used for interpretation of resistance, and phylogenetic analysis was performed for subtype assignment. Results: In the PR gene, no antiviral resistance-associated mutation was detected. For RT-associated mutations, K103N was the most prevalent in sequenced samples (14.3%). Genetic subtyping on the pol gene revealed that the majority of the prisoners were infected with subtype CRF33_01B (52.4%). Conclusion: Continuous surveillance of newly infected individuals is required to help strategize the best antiviral treatment for these patients. https://jidc.org/index.php/journal/article/view/4095HIV-1CRF33_01Bdrug resistanceprisoners |
| spellingShingle | Tengku Ahmad Akram Tengku Mohd Ariffin Suharni Mohamad Wan Nazirah Wan Yusuf Rafidah Hanim Shueb Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia Journal of Infection in Developing Countries HIV-1 CRF33_01B drug resistance prisoners |
| title | Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia |
| title_full | Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia |
| title_fullStr | Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia |
| title_full_unstemmed | Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia |
| title_short | Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia |
| title_sort | antiretroviral drug resistance and hiv 1 subtypes among treatment naive prisoners in kelantan malaysia |
| topic | HIV-1 CRF33_01B drug resistance prisoners |
| url | https://jidc.org/index.php/journal/article/view/4095 |
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