Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems

ABSTRACT (1) Background: Vaccine programs have been failing to provide complete immunity against the Newcastle disease virus (NDV). This study examines the immunological responses to the live LaSota and Avinew (VG/GA) vaccines given through spray or drinking water to broiler chickens; (2) Methods:...

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Main Authors: SM Albarrak, YM Alharbi, FA Alshanbari, FAM Abo-Aziza, TI Almundarij, S Altuwaijri, AKA Zaki
Format: Article
Language:English
Published: Fundação APINCO de Ciência e Tecnologia Avícolas 2025-03-01
Series:Brazilian Journal of Poultry Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2025000100302&lng=en&tlng=en
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author SM Albarrak
YM Alharbi
FA Alshanbari
FAM Abo-Aziza
TI Almundarij
S Altuwaijri
AKA Zaki
author_facet SM Albarrak
YM Alharbi
FA Alshanbari
FAM Abo-Aziza
TI Almundarij
S Altuwaijri
AKA Zaki
author_sort SM Albarrak
collection DOAJ
description ABSTRACT (1) Background: Vaccine programs have been failing to provide complete immunity against the Newcastle disease virus (NDV). This study examines the immunological responses to the live LaSota and Avinew (VG/GA) vaccines given through spray or drinking water to broiler chickens; (2) Methods: The experimental vaccination included one unvaccinated control group and four other groups of the same size. There were three duplicates, each with 30 birds, with each group amounting to ninety birds. In addition to the status of the cellular and humoral immunity, we examined the weights and histology of the bursa and spleen; (3) Results: The bursa weight index (BWI) significantly increased on days 28 and 42 in the vaccinated groups, regardless of the vaccination method (p≤0.05). The spleen weight index (SWI) significantly increased on day 42 in VG/GA vaccinated birds, and on day 28 in chicks vaccinated with the LaSota strain (p≤0.05). T cell proliferation in response to stimulation with PHA was significantly higher across all vaccinated groups compared to the unvaccinated ones. Serum IgG was significantly elevated on days 21, 28, and 42 post-vaccination with VG/GA or LaSota strain for both vaccination routes (p≤0.05); (4) Conclusions: The data generated from this study demonstrated that significant immune responses can be generated with the two examined Newcastle vaccine strains using the spray and drinking water vaccination routes.
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spelling doaj-art-e641710d9ec545008e9bebebb55631142025-08-20T02:26:01ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612025-03-0127110.1590/1806-9061-2023-1883Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery SystemsSM Albarrakhttps://orcid.org/0000-0003-4965-6142YM Alharbihttps://orcid.org/0000-0003-1658-0153FA Alshanbarihttps://orcid.org/0000-0001-7267-4301FAM Abo-Azizahttps://orcid.org/0000-0003-2271-2239TI Almundarijhttps://orcid.org/0000-0001-6228-297XS Altuwaijrihttps://orcid.org/0000-0002-2582-7771AKA Zakihttps://orcid.org/0000-0001-7616-0789ABSTRACT (1) Background: Vaccine programs have been failing to provide complete immunity against the Newcastle disease virus (NDV). This study examines the immunological responses to the live LaSota and Avinew (VG/GA) vaccines given through spray or drinking water to broiler chickens; (2) Methods: The experimental vaccination included one unvaccinated control group and four other groups of the same size. There were three duplicates, each with 30 birds, with each group amounting to ninety birds. In addition to the status of the cellular and humoral immunity, we examined the weights and histology of the bursa and spleen; (3) Results: The bursa weight index (BWI) significantly increased on days 28 and 42 in the vaccinated groups, regardless of the vaccination method (p≤0.05). The spleen weight index (SWI) significantly increased on day 42 in VG/GA vaccinated birds, and on day 28 in chicks vaccinated with the LaSota strain (p≤0.05). T cell proliferation in response to stimulation with PHA was significantly higher across all vaccinated groups compared to the unvaccinated ones. Serum IgG was significantly elevated on days 21, 28, and 42 post-vaccination with VG/GA or LaSota strain for both vaccination routes (p≤0.05); (4) Conclusions: The data generated from this study demonstrated that significant immune responses can be generated with the two examined Newcastle vaccine strains using the spray and drinking water vaccination routes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2025000100302&lng=en&tlng=enAntioxidant enzymesAvinewELISAimmunityLaSotaross chickensvaccine
spellingShingle SM Albarrak
YM Alharbi
FA Alshanbari
FAM Abo-Aziza
TI Almundarij
S Altuwaijri
AKA Zaki
Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems
Brazilian Journal of Poultry Science
Antioxidant enzymes
Avinew
ELISA
immunity
LaSota
ross chickens
vaccine
title Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems
title_full Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems
title_fullStr Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems
title_full_unstemmed Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems
title_short Assessment of Immunological Responses of Broilers Vaccinated with Newcastle Strains Using Either Spray or Water Delivery Systems
title_sort assessment of immunological responses of broilers vaccinated with newcastle strains using either spray or water delivery systems
topic Antioxidant enzymes
Avinew
ELISA
immunity
LaSota
ross chickens
vaccine
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2025000100302&lng=en&tlng=en
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