Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles
Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play a significant role in cancer development as regulators of protein-coding genes. Their dysregulation was in some extent already associated with glioma, the most aggressive primary brain tumours in adults. The correct diagnosis and treatment se...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/2312318 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849467779807182848 |
|---|---|
| author | Alenka Matjašič Mojca Tajnik Emanuela Boštjančič Mara Popović Boštjan Matos Damjan Glavač |
| author_facet | Alenka Matjašič Mojca Tajnik Emanuela Boštjančič Mara Popović Boštjan Matos Damjan Glavač |
| author_sort | Alenka Matjašič |
| collection | DOAJ |
| description | Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play a significant role in cancer development as regulators of protein-coding genes. Their dysregulation was in some extent already associated with glioma, the most aggressive primary brain tumours in adults. The correct diagnosis and treatment selection due to high tumour heterogeneity might be difficult and inadequate, resulting in poor prognosis. Studies of expression patterns of noncoding RNAs (ncRNAs) could provide useful insight in glioma molecular development. We used the qPCR approach to screen and investigate the expression of lncRNAs that were previously deregulated in other cancer types. The study showed altered expression levels for numerous lncRNAs across histologically different glioma samples. Validation of few lncRNAs showed association of expression levels with histological subtype and/or malignancy grade. We also observed deregulated and subtype-distinctive expression for four lncRNA-associated miRNAs. Expression of few lncRNAs and miRNA was also associated with patients’ survival, showing potential prognostic value. Several ncRNAs, some already related to glioma and some, to the best of our knowledge, investigated for the first time, might be of greater importance in glioma molecular development and progression. Finding the subtype-specific lncRNA and/or miRNA expression patterns may contribute additional information for a more objective classification. |
| format | Article |
| id | doaj-art-e63d804a0d45458ea3a75633937af5ff |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-e63d804a0d45458ea3a75633937af5ff2025-08-20T03:26:04ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/23123182312318Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression ProfilesAlenka Matjašič0Mojca Tajnik1Emanuela Boštjančič2Mara Popović3Boštjan Matos4Damjan Glavač5Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaDepartment of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaDepartment of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaInstitute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaDepartment of Neurosurgery, University Medical Centre, Ljubljana, SloveniaDepartment of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaLong noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play a significant role in cancer development as regulators of protein-coding genes. Their dysregulation was in some extent already associated with glioma, the most aggressive primary brain tumours in adults. The correct diagnosis and treatment selection due to high tumour heterogeneity might be difficult and inadequate, resulting in poor prognosis. Studies of expression patterns of noncoding RNAs (ncRNAs) could provide useful insight in glioma molecular development. We used the qPCR approach to screen and investigate the expression of lncRNAs that were previously deregulated in other cancer types. The study showed altered expression levels for numerous lncRNAs across histologically different glioma samples. Validation of few lncRNAs showed association of expression levels with histological subtype and/or malignancy grade. We also observed deregulated and subtype-distinctive expression for four lncRNA-associated miRNAs. Expression of few lncRNAs and miRNA was also associated with patients’ survival, showing potential prognostic value. Several ncRNAs, some already related to glioma and some, to the best of our knowledge, investigated for the first time, might be of greater importance in glioma molecular development and progression. Finding the subtype-specific lncRNA and/or miRNA expression patterns may contribute additional information for a more objective classification.http://dx.doi.org/10.1155/2017/2312318 |
| spellingShingle | Alenka Matjašič Mojca Tajnik Emanuela Boštjančič Mara Popović Boštjan Matos Damjan Glavač Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles International Journal of Genomics |
| title | Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles |
| title_full | Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles |
| title_fullStr | Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles |
| title_full_unstemmed | Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles |
| title_short | Identifying Novel Glioma-Associated Noncoding RNAs by Their Expression Profiles |
| title_sort | identifying novel glioma associated noncoding rnas by their expression profiles |
| url | http://dx.doi.org/10.1155/2017/2312318 |
| work_keys_str_mv | AT alenkamatjasic identifyingnovelgliomaassociatednoncodingrnasbytheirexpressionprofiles AT mojcatajnik identifyingnovelgliomaassociatednoncodingrnasbytheirexpressionprofiles AT emanuelabostjancic identifyingnovelgliomaassociatednoncodingrnasbytheirexpressionprofiles AT marapopovic identifyingnovelgliomaassociatednoncodingrnasbytheirexpressionprofiles AT bostjanmatos identifyingnovelgliomaassociatednoncodingrnasbytheirexpressionprofiles AT damjanglavac identifyingnovelgliomaassociatednoncodingrnasbytheirexpressionprofiles |