SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth
Abstract The Arabidopsis seor1ko line, which expresses the protein AtSEOR2 free of its bond with AtSEOR1, exhibits a lower phytoplasma titre as compared to wild type plants. In search for mechanism(s) underlying potential SEOR2-mediated defense responses the transcriptome of healthy wild type and At...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-01374-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849325684574388224 |
|---|---|
| author | Keziah Omenge Ottone Carmelo Viscardo Fernando Rodrigo De Oliveira Cantao Simonetta Santi Aart Jan Eeuwe van Bel Rita Musetti |
| author_facet | Keziah Omenge Ottone Carmelo Viscardo Fernando Rodrigo De Oliveira Cantao Simonetta Santi Aart Jan Eeuwe van Bel Rita Musetti |
| author_sort | Keziah Omenge |
| collection | DOAJ |
| description | Abstract The Arabidopsis seor1ko line, which expresses the protein AtSEOR2 free of its bond with AtSEOR1, exhibits a lower phytoplasma titre as compared to wild type plants. In search for mechanism(s) underlying potential SEOR2-mediated defense responses the transcriptome of healthy wild type and Atseor1ko plants was disclosed by RNA sequencing. Comparative transcriptome analysis revealed 1036 differentially expressed genes (DEGs, 893 up- and 143 down-regulated) between the Atseor1ko line and the wild type. Sequence annotation and classification of the up-regulated genes identified “plant-pathogen interaction” among the most enriched clusters. The “plant-pathogen interaction” cluster included genes encoding members of the protein kinase superfamily, actors in calcium/calmodulin signaling transduction and WRKY transcription factors. An interaction network analysis and a host-phytoplasma interaction map demonstrated that AtSEOR2 protein could interact with the calcium-binding proteins CAM2 and TCH3. The latter one also turned out to be an indirect target of the SAP54CY phytoplasma effector, which suggests a SEOR2-mediated role of TCH3 in balancing nutrient investments in plant defense and plant growth. |
| format | Article |
| id | doaj-art-e62537f924df439bb7dd1ba4da3d1935 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e62537f924df439bb7dd1ba4da3d19352025-08-20T03:48:19ZengNature PortfolioScientific Reports2045-23222025-05-0115111910.1038/s41598-025-01374-8SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growthKeziah Omenge0Ottone Carmelo Viscardo1Fernando Rodrigo De Oliveira Cantao2Simonetta Santi3Aart Jan Eeuwe van Bel4Rita Musetti5Institute for Biosafety in Plant Biotechnology (SB), Julius Kühn-Institut (JKI) - Federal Research Centre for Cultivated PlantsDepartment of Land, Environment, Agriculture and Forestry (TESAF), University of PadovaDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206Institute of Phytopathology, Justus-Liebig UniversityDepartment of Land, Environment, Agriculture and Forestry (TESAF), University of PadovaAbstract The Arabidopsis seor1ko line, which expresses the protein AtSEOR2 free of its bond with AtSEOR1, exhibits a lower phytoplasma titre as compared to wild type plants. In search for mechanism(s) underlying potential SEOR2-mediated defense responses the transcriptome of healthy wild type and Atseor1ko plants was disclosed by RNA sequencing. Comparative transcriptome analysis revealed 1036 differentially expressed genes (DEGs, 893 up- and 143 down-regulated) between the Atseor1ko line and the wild type. Sequence annotation and classification of the up-regulated genes identified “plant-pathogen interaction” among the most enriched clusters. The “plant-pathogen interaction” cluster included genes encoding members of the protein kinase superfamily, actors in calcium/calmodulin signaling transduction and WRKY transcription factors. An interaction network analysis and a host-phytoplasma interaction map demonstrated that AtSEOR2 protein could interact with the calcium-binding proteins CAM2 and TCH3. The latter one also turned out to be an indirect target of the SAP54CY phytoplasma effector, which suggests a SEOR2-mediated role of TCH3 in balancing nutrient investments in plant defense and plant growth.https://doi.org/10.1038/s41598-025-01374-8ArabidopsisAtSEOR2EffectorsInteractionPhloemPlant defense |
| spellingShingle | Keziah Omenge Ottone Carmelo Viscardo Fernando Rodrigo De Oliveira Cantao Simonetta Santi Aart Jan Eeuwe van Bel Rita Musetti SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth Scientific Reports Arabidopsis AtSEOR2 Effectors Interaction Phloem Plant defense |
| title | SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth |
| title_full | SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth |
| title_fullStr | SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth |
| title_full_unstemmed | SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth |
| title_short | SEOR2 in Arabidopsis mediates Ca2+ dependent defense against phytoplasmas and reduction of plant growth |
| title_sort | seor2 in arabidopsis mediates ca2 dependent defense against phytoplasmas and reduction of plant growth |
| topic | Arabidopsis AtSEOR2 Effectors Interaction Phloem Plant defense |
| url | https://doi.org/10.1038/s41598-025-01374-8 |
| work_keys_str_mv | AT keziahomenge seor2inarabidopsismediatesca2dependentdefenseagainstphytoplasmasandreductionofplantgrowth AT ottonecarmeloviscardo seor2inarabidopsismediatesca2dependentdefenseagainstphytoplasmasandreductionofplantgrowth AT fernandorodrigodeoliveiracantao seor2inarabidopsismediatesca2dependentdefenseagainstphytoplasmasandreductionofplantgrowth AT simonettasanti seor2inarabidopsismediatesca2dependentdefenseagainstphytoplasmasandreductionofplantgrowth AT aartjaneeuwevanbel seor2inarabidopsismediatesca2dependentdefenseagainstphytoplasmasandreductionofplantgrowth AT ritamusetti seor2inarabidopsismediatesca2dependentdefenseagainstphytoplasmasandreductionofplantgrowth |