Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt
Introduction: Bovine viral diarrhea (BVD) is one of the most economically significant diseases in the bovine industry causing losses due to diarrhea, reproductive disorders, immunosuppression and mortalities. The aim of our investigation was to detect and subtype BVDV from calves on two dairy cattle...
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The Journal of Infection in Developing Countries
2015-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/7259 |
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| author | Mohamed Ahmed Soltan Rebecca P Wilkes Mohamed Nagy Elsheery Mahmoud Mohy Elhaig Matthhew C Riley Melissa A Kennedy |
| author_facet | Mohamed Ahmed Soltan Rebecca P Wilkes Mohamed Nagy Elsheery Mahmoud Mohy Elhaig Matthhew C Riley Melissa A Kennedy |
| author_sort | Mohamed Ahmed Soltan |
| collection | DOAJ |
| description | Introduction: Bovine viral diarrhea (BVD) is one of the most economically significant diseases in the bovine industry causing losses due to diarrhea, reproductive disorders, immunosuppression and mortalities. The aim of our investigation was to detect and subtype BVDV from calves on two dairy cattle and two buffalo farms in Ismailia province, Egypt as an indicator of BVDV infection status in the province.
Methodology: A total of 298 blood samples were collected and tested using an optimized one-step, real-time multiplex Taqman-based RT-PCR. All the positive samples by the multiplex real-time RT-PCR were tested using conventional RT-PCR to amplify multiple areas of the genome for further phylogenetic analysis and subtyping.
Results: Thirty one (10.4%) of the tested samples were positive for BVDV-1. Only three samples, all from a single dairy cattle farm, had enough viral RNA to be amplified by RT-PCR. The PCR products were sequenced and phylogenetic analysis revealed detection of BVDV-1b. The detected strain is closely related to worldwide BVDV-1b strains, making it difficult to trace its origin. Nucleotide and amino acid alignments of the E2 glycoprotein region of the detected strain with other BVDV-1b strains showed high divergence, with identity ranging from 81.3% to 93.6% and 85.3% to 93.6%, respectively.
Conclusion: To our knowledge, this is the first report describing the circulation of BVDV-1b in Egyptian dairy cattle populations.
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| format | Article |
| id | doaj-art-e62266f35cde4dea93df3693b920740b |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e62266f35cde4dea93df3693b920740b2025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-12-0191210.3855/jidc.7259Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, EgyptMohamed Ahmed Soltan0Rebecca P Wilkes1Mohamed Nagy Elsheery2Mahmoud Mohy Elhaig3Matthhew C Riley4Melissa A Kennedy5Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptCollege of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, United StatesFaculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptFaculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptCollege of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, United StatesCollege of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, United StatesIntroduction: Bovine viral diarrhea (BVD) is one of the most economically significant diseases in the bovine industry causing losses due to diarrhea, reproductive disorders, immunosuppression and mortalities. The aim of our investigation was to detect and subtype BVDV from calves on two dairy cattle and two buffalo farms in Ismailia province, Egypt as an indicator of BVDV infection status in the province. Methodology: A total of 298 blood samples were collected and tested using an optimized one-step, real-time multiplex Taqman-based RT-PCR. All the positive samples by the multiplex real-time RT-PCR were tested using conventional RT-PCR to amplify multiple areas of the genome for further phylogenetic analysis and subtyping. Results: Thirty one (10.4%) of the tested samples were positive for BVDV-1. Only three samples, all from a single dairy cattle farm, had enough viral RNA to be amplified by RT-PCR. The PCR products were sequenced and phylogenetic analysis revealed detection of BVDV-1b. The detected strain is closely related to worldwide BVDV-1b strains, making it difficult to trace its origin. Nucleotide and amino acid alignments of the E2 glycoprotein region of the detected strain with other BVDV-1b strains showed high divergence, with identity ranging from 81.3% to 93.6% and 85.3% to 93.6%, respectively. Conclusion: To our knowledge, this is the first report describing the circulation of BVDV-1b in Egyptian dairy cattle populations. https://jidc.org/index.php/journal/article/view/7259Bovine viral diarrhea virustypingphylogenetic analysisBVDV-1bEgypt |
| spellingShingle | Mohamed Ahmed Soltan Rebecca P Wilkes Mohamed Nagy Elsheery Mahmoud Mohy Elhaig Matthhew C Riley Melissa A Kennedy Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt Journal of Infection in Developing Countries Bovine viral diarrhea virus typing phylogenetic analysis BVDV-1b Egypt |
| title | Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt |
| title_full | Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt |
| title_fullStr | Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt |
| title_full_unstemmed | Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt |
| title_short | Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt |
| title_sort | circulation of bovine viral diarrhea virus 1 bvdv 1 in dairy cattle and buffalo farms in ismailia province egypt |
| topic | Bovine viral diarrhea virus typing phylogenetic analysis BVDV-1b Egypt |
| url | https://jidc.org/index.php/journal/article/view/7259 |
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