Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo
Fumonisin B1 (FB1) is a pervasive hazardous substance in the environment, presenting significant threats to human health and ecological systems. Propyl gallate (PG) is an antioxidant widely used in foods, cosmetics and pharmaceuticals, and its occurrence is an emerging issue in environmental chemist...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325007808 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849694405728927744 |
|---|---|
| author | Xiangrong Chen Chenxin Dongye Yan Li Yanfang Zhao Rabah Boukherroub Huijuan Li Tianliang Li Xiangfeng Chen |
| author_facet | Xiangrong Chen Chenxin Dongye Yan Li Yanfang Zhao Rabah Boukherroub Huijuan Li Tianliang Li Xiangfeng Chen |
| author_sort | Xiangrong Chen |
| collection | DOAJ |
| description | Fumonisin B1 (FB1) is a pervasive hazardous substance in the environment, presenting significant threats to human health and ecological systems. Propyl gallate (PG) is an antioxidant widely used in foods, cosmetics and pharmaceuticals, and its occurrence is an emerging issue in environmental chemistry. These two common substances in foods are easily ingested by people and closely related to the human gut. The aim of the current study was to identify the combined effect of PG on FB1 in vivo and in vitro. In the work, it is found that PG reduced colon inflamation and production of inflammatory cytokines induced by FB1 in mice and Raw 264.7 cells. Proteomics and metabolomics clearly showed differentially expressed proteins and metabolites in the comparison between FB1-PG and FB1 groups, which revealed a particular focus on the Akr1b8 protein in NF-κB signaling activity, the metabolic processes of nucleotide metabolism and ABC transporters. Comparative proteomic and metabolomic analysis revealed that Akr1b8 protein was positively correlated with glycerol and negatively correlated with LysoPC (24:1(15Z)) in the comparison of FB1 and FB1-PG. These findings provide important insights into the understanding of PG and FB1 interactions in humans and provide potential targets for the development of novel therapeutic strategies against colon inflammation. |
| format | Article |
| id | doaj-art-e61d6913d4894bc3b23a9cb2a3b7fc62 |
| institution | DOAJ |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e61d6913d4894bc3b23a9cb2a3b7fc622025-08-20T03:20:05ZengElsevierEcotoxicology and Environmental Safety0147-65132025-07-0130011844010.1016/j.ecoenv.2025.118440Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivoXiangrong Chen0Chenxin Dongye1Yan Li2Yanfang Zhao3Rabah Boukherroub4Huijuan Li5Tianliang Li6Xiangfeng Chen7Shandong International Joint Laboratory of Environmental and Biological Science, Qilu University of Technology (Shandong Academy of Science), Jinan, Shandong 250014, ChinaShandong International Joint Laboratory of Environmental and Biological Science, Qilu University of Technology (Shandong Academy of Science), Jinan, Shandong 250014, ChinaCenter for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, College of Life Sciences, Shandong Normal University, Jinan 250358, ChinaShandong International Joint Laboratory of Environmental and Biological Science, Qilu University of Technology (Shandong Academy of Science), Jinan, Shandong 250014, ChinaUniv. Lille, CNRS, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille F-59000, FranceShandong International Joint Laboratory of Environmental and Biological Science, Qilu University of Technology (Shandong Academy of Science), Jinan, Shandong 250014, ChinaCenter for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, College of Life Sciences, Shandong Normal University, Jinan 250358, China; Correspondence to: Shandong Normal University, 534 Bowu Building, Jinan 250358, China.Shandong International Joint Laboratory of Environmental and Biological Science, Qilu University of Technology (Shandong Academy of Science), Jinan, Shandong 250014, China; Correspondence to: Qilu University of Technology (Shandong Academy of Science), Shandong Analysis and Test Centre, Jinan, Shandong 250014, China.Fumonisin B1 (FB1) is a pervasive hazardous substance in the environment, presenting significant threats to human health and ecological systems. Propyl gallate (PG) is an antioxidant widely used in foods, cosmetics and pharmaceuticals, and its occurrence is an emerging issue in environmental chemistry. These two common substances in foods are easily ingested by people and closely related to the human gut. The aim of the current study was to identify the combined effect of PG on FB1 in vivo and in vitro. In the work, it is found that PG reduced colon inflamation and production of inflammatory cytokines induced by FB1 in mice and Raw 264.7 cells. Proteomics and metabolomics clearly showed differentially expressed proteins and metabolites in the comparison between FB1-PG and FB1 groups, which revealed a particular focus on the Akr1b8 protein in NF-κB signaling activity, the metabolic processes of nucleotide metabolism and ABC transporters. Comparative proteomic and metabolomic analysis revealed that Akr1b8 protein was positively correlated with glycerol and negatively correlated with LysoPC (24:1(15Z)) in the comparison of FB1 and FB1-PG. These findings provide important insights into the understanding of PG and FB1 interactions in humans and provide potential targets for the development of novel therapeutic strategies against colon inflammation.http://www.sciencedirect.com/science/article/pii/S0147651325007808Fumonisin B1Propyl gallateColon inflammationProteomicsMetabolomicsAkr1b8 protein |
| spellingShingle | Xiangrong Chen Chenxin Dongye Yan Li Yanfang Zhao Rabah Boukherroub Huijuan Li Tianliang Li Xiangfeng Chen Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo Ecotoxicology and Environmental Safety Fumonisin B1 Propyl gallate Colon inflammation Proteomics Metabolomics Akr1b8 protein |
| title | Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo |
| title_full | Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo |
| title_fullStr | Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo |
| title_full_unstemmed | Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo |
| title_short | Integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin B1 on colon inflammation in vitro and in vivo |
| title_sort | integrated proteomics and metabolomics to evaluate the combined effect of propyl gallate and fumonisin b1 on colon inflammation in vitro and in vivo |
| topic | Fumonisin B1 Propyl gallate Colon inflammation Proteomics Metabolomics Akr1b8 protein |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325007808 |
| work_keys_str_mv | AT xiangrongchen integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT chenxindongye integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT yanli integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT yanfangzhao integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT rabahboukherroub integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT huijuanli integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT tianliangli integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo AT xiangfengchen integratedproteomicsandmetabolomicstoevaluatethecombinedeffectofpropylgallateandfumonisinb1oncoloninflammationinvitroandinvivo |