Protocol to generate genetically engineered organoid-initiated mouse models of esophageal cancer

Protocol to generate genetically engineered organoid-initiated mouse models of esophageal cancer

Summary: Here, we present a protocol for generating genetically engineered organoid-initiated mouse models of esophageal cancer. We describe steps for isolating mouse esophageal primary cells, organoids culture, construct and virus preparation, and genome editing. We then detail procedures for trans...

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Bibliographic Details
Main Authors: Jian Wang, Jiajia Du, Xiangmeng Luo, Linjie Guo, Xiang Li, Ziling Dong, Zhenghao Lu, Xudong Wan, Lu Wang, Hongyu Liu, He Li, Ping Tan, Yanhong Ji, Yuan Li, Yaxin Li, Yushang Yang, Yong Yuan, Longqi Chen, Bing Hu, Yu Liu, Feifei Na, Chong Chen
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cancer
Model Organisms
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004344
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Summary:Summary: Here, we present a protocol for generating genetically engineered organoid-initiated mouse models of esophageal cancer. We describe steps for isolating mouse esophageal primary cells, organoids culture, construct and virus preparation, and genome editing. We then detail procedures for transplanting engineered organoids into the esophagus, forming squamous cell carcinoma, and tumorigenesis monitoring. The protocol is faster than genetically engineered mouse models.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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