C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway
Skeletal muscle development is closely related with the amount of meat production and its quality in chickens. Natriuretic peptides (NPs) play an important role in myotube formation and fat oxidation of skeletal muscle in animals. The effect of C-type natriuretic peptide (CNP), an important member o...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2022-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921636948 |
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| author | Hua-yun HUANG Zhong LIANG Long-zhou LIU Chun-miao LI Zhen-yang HUANG Qian-bao WANG Shou-feng LI Zhen-hua ZHAO |
| author_facet | Hua-yun HUANG Zhong LIANG Long-zhou LIU Chun-miao LI Zhen-yang HUANG Qian-bao WANG Shou-feng LI Zhen-hua ZHAO |
| author_sort | Hua-yun HUANG |
| collection | DOAJ |
| description | Skeletal muscle development is closely related with the amount of meat production and its quality in chickens. Natriuretic peptides (NPs) play an important role in myotube formation and fat oxidation of skeletal muscle in animals. The effect of C-type natriuretic peptide (CNP), an important member of the NPs, and its underlying molecular mechanisms in skeletal muscle are incompletely understood. Treatment of myoblasts with CNP led to enhanced proliferation/differentiation and significantly upregulated (P<0.05) mRNA expression of the CNP receptors natriuretic peptide receptor B (NPRB) and the clearance receptor C (NPRC). In cells exposed to CNP, 142 differentially expressed genes (84 up-regulation and 58 down-regulation) (P<0.05) were identified by RNA-sequencing compared with those in control cells. Sixteen genes were significantly enriched (P<0.05) in the metabolic pathway, and six of them (phospholipase C β4, phospholipase C β2, phosphoglycerate mutase 1, creatine kinase B, peroxiredoxin 6 and CD38) were closely related to skeletal muscle development and differentially expressed. In conclusion, CNP stimulated differentiation of myoblasts by upregulating expression of the NPRB and NPRC receptors and enriching key genes in the metabolic pathway. |
| format | Article |
| id | doaj-art-e619761cb7794aff9a28e9bcd1f40ccc |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e619761cb7794aff9a28e9bcd1f40ccc2025-08-20T03:57:59ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192022-02-0121249650310.1016/S2095-3119(21)63694-8C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathwayHua-yun HUANG0Zhong LIANG1Long-zhou LIU2Chun-miao LI3Zhen-yang HUANG4Qian-bao WANG5Shou-feng LI6Zhen-hua ZHAO7Jiangsu Institute of Poultry Science, Yangzhou 225125, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.China; College of Animal Science, Yangtze University, Jingzhou 8060550, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.ChinaJiangsu Institute of Poultry Science, Yangzhou 225125, P.R.China; Correspondence ZHAO Zhen-hua, Tel/Fax: +86-514-85599073Skeletal muscle development is closely related with the amount of meat production and its quality in chickens. Natriuretic peptides (NPs) play an important role in myotube formation and fat oxidation of skeletal muscle in animals. The effect of C-type natriuretic peptide (CNP), an important member of the NPs, and its underlying molecular mechanisms in skeletal muscle are incompletely understood. Treatment of myoblasts with CNP led to enhanced proliferation/differentiation and significantly upregulated (P<0.05) mRNA expression of the CNP receptors natriuretic peptide receptor B (NPRB) and the clearance receptor C (NPRC). In cells exposed to CNP, 142 differentially expressed genes (84 up-regulation and 58 down-regulation) (P<0.05) were identified by RNA-sequencing compared with those in control cells. Sixteen genes were significantly enriched (P<0.05) in the metabolic pathway, and six of them (phospholipase C β4, phospholipase C β2, phosphoglycerate mutase 1, creatine kinase B, peroxiredoxin 6 and CD38) were closely related to skeletal muscle development and differentially expressed. In conclusion, CNP stimulated differentiation of myoblasts by upregulating expression of the NPRB and NPRC receptors and enriching key genes in the metabolic pathway.http://www.sciencedirect.com/science/article/pii/S2095311921636948CNPNPRB/NPRC receptormyoblast differentiationmetabolism pathwaychicken |
| spellingShingle | Hua-yun HUANG Zhong LIANG Long-zhou LIU Chun-miao LI Zhen-yang HUANG Qian-bao WANG Shou-feng LI Zhen-hua ZHAO C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway Journal of Integrative Agriculture CNP NPRB/NPRC receptor myoblast differentiation metabolism pathway chicken |
| title | C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway |
| title_full | C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway |
| title_fullStr | C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway |
| title_full_unstemmed | C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway |
| title_short | C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway |
| title_sort | c type natriuretic peptide stimulates chicken myoblast differentiation through nprb nprc receptors and metabolism pathway |
| topic | CNP NPRB/NPRC receptor myoblast differentiation metabolism pathway chicken |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921636948 |
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