Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa
The overwhelming challenges of climate change and increased population trigger the roar of food productivity. Untamed environmental stress conditions have become a harsh reality in the sustainable development of agriculture. This scenario has led to the regularization of fertilizers in agriculture,...
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Action for Sustainable Efficacious Development and Awareness
2025-04-01
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| Series: | Environment Conservation Journal |
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| Online Access: | https://journal.environcj.in/index.php/ecj/article/view/3026 |
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| author | Divya Bhagat Shiwani Guleria Sharma Praveen Guleria Vineet Kumar |
| author_facet | Divya Bhagat Shiwani Guleria Sharma Praveen Guleria Vineet Kumar |
| author_sort | Divya Bhagat |
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The overwhelming challenges of climate change and increased population trigger the roar of food productivity. Untamed environmental stress conditions have become a harsh reality in the sustainable development of agriculture. This scenario has led to the regularization of fertilizers in agriculture, which has further raised concerns. Given this, the present study reports the isolation of ACC deaminase-producing rhizobacteria from the stress-prone crop Oryza sativa from Punjab, India, targeting a sustainable solution for agriculture. A total of 50 isolates were identified from 9 districts of Punjab by primary screening on ACC-supplemented DF media. Further, all the isolates were quantified for ACC deaminase production, and the highest production was observed for CJSKII3, i.e., 0.89±0.02 µM/mg protein/h, and the minimum production was noted for NGNI, i.e., 0.52±0.0 µM/mg protein/h. Out of 50 isolates, 6% of rhizobacteria were able to produce IAA, 74% for biofilm production, 38% for nitrogen fixation, 40% for siderophore production, 12% for HCN production, and 54% for phosphate solubilization. They were also identified for 16% protease production, 22% amylase production, and 52% rhizobacteria showed antifungal activity against the fungus Fusarium oxysporum. Three rhizobacteria, i.e., CJSKII3, NARI2, and NARI4, showed prominent results with maximum PGPR activities. All three were identified by 16S rRNA molecular sequencing as Bacillus tropicus, Alcaligenes aquatilis, and Alcaligenes faecalis. As a promising stress-diminishing trait, these ACC deaminase-producing PGPRs can further be assessed in agriculture as an effective biofertilizer.
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| format | Article |
| id | doaj-art-e61759e88f8b473cb64da5c65d10a453 |
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| issn | 0972-3099 2278-5124 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Action for Sustainable Efficacious Development and Awareness |
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| series | Environment Conservation Journal |
| spelling | doaj-art-e61759e88f8b473cb64da5c65d10a4532025-08-20T03:22:44ZengAction for Sustainable Efficacious Development and AwarenessEnvironment Conservation Journal0972-30992278-51242025-04-0126210.36953/ECJ.30262967Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativaDivya Bhagat0Shiwani Guleria Sharma1Praveen Guleria2Vineet Kumar3Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, IndiaDepartment of Microbiology, Punjab Agriculture University, Ludhiana, Punjab, IndiaDepartment of Biotechnology, DAV University, Jalandhar, Punjab, IndiaDepartment of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India The overwhelming challenges of climate change and increased population trigger the roar of food productivity. Untamed environmental stress conditions have become a harsh reality in the sustainable development of agriculture. This scenario has led to the regularization of fertilizers in agriculture, which has further raised concerns. Given this, the present study reports the isolation of ACC deaminase-producing rhizobacteria from the stress-prone crop Oryza sativa from Punjab, India, targeting a sustainable solution for agriculture. A total of 50 isolates were identified from 9 districts of Punjab by primary screening on ACC-supplemented DF media. Further, all the isolates were quantified for ACC deaminase production, and the highest production was observed for CJSKII3, i.e., 0.89±0.02 µM/mg protein/h, and the minimum production was noted for NGNI, i.e., 0.52±0.0 µM/mg protein/h. Out of 50 isolates, 6% of rhizobacteria were able to produce IAA, 74% for biofilm production, 38% for nitrogen fixation, 40% for siderophore production, 12% for HCN production, and 54% for phosphate solubilization. They were also identified for 16% protease production, 22% amylase production, and 52% rhizobacteria showed antifungal activity against the fungus Fusarium oxysporum. Three rhizobacteria, i.e., CJSKII3, NARI2, and NARI4, showed prominent results with maximum PGPR activities. All three were identified by 16S rRNA molecular sequencing as Bacillus tropicus, Alcaligenes aquatilis, and Alcaligenes faecalis. As a promising stress-diminishing trait, these ACC deaminase-producing PGPRs can further be assessed in agriculture as an effective biofertilizer. https://journal.environcj.in/index.php/ecj/article/view/3026ACC deaminase CharacterizationPGPR Rhizobacteria Rice |
| spellingShingle | Divya Bhagat Shiwani Guleria Sharma Praveen Guleria Vineet Kumar Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa Environment Conservation Journal ACC deaminase Characterization PGPR Rhizobacteria Rice |
| title | Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa |
| title_full | Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa |
| title_fullStr | Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa |
| title_full_unstemmed | Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa |
| title_short | Isolation of ACC-deaminase producing plant growth promoting rhizobacteria from Oryza sativa |
| title_sort | isolation of acc deaminase producing plant growth promoting rhizobacteria from oryza sativa |
| topic | ACC deaminase Characterization PGPR Rhizobacteria Rice |
| url | https://journal.environcj.in/index.php/ecj/article/view/3026 |
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