Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques
To investigate the differences in bacterial community structure between high- and medium-high-temperature Daqu, the bacterial community structure of the two types of Daqu was analyzed by third-generation sequencing technology, the correlation between bacterial community and gene functions of bacteri...
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Editorial Department of China Brewing
2025-05-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-5-146.pdf |
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| author | WANG Wenhang, CAI Wenchao, YU Peirong, CHEN Wei, SHAN Chunhui, GUO Zhuang, WANG Yurong |
| author_facet | WANG Wenhang, CAI Wenchao, YU Peirong, CHEN Wei, SHAN Chunhui, GUO Zhuang, WANG Yurong |
| author_sort | WANG Wenhang, CAI Wenchao, YU Peirong, CHEN Wei, SHAN Chunhui, GUO Zhuang, WANG Yurong |
| collection | DOAJ |
| description | To investigate the differences in bacterial community structure between high- and medium-high-temperature Daqu, the bacterial community structure of the two types of Daqu was analyzed by third-generation sequencing technology, the correlation between bacterial community and gene functions of bacterial community were analyzed, and the strains were isolated and identified by microbial pure culture and molecular biology techniques. The results showed that the dominant bacterial phyla (average relative abundance>1%) in two types of Daqu included Firmicutes, and the dominant bacterial genera included Kroppenstedtia, and the dominant bacterial species included Kroppenstedtia eburnea. Principal coordinates analysis (PCoA) and linear discriminant analysis effect size (LEfSe) analysis results showed that there were significant differences in the bacterial community structure between the two types of Daqu, with Thermoactinomycetaceae and Dietziaceae as biomarkers for high- and medium-high-temperature Daqu, respectively. Correlation analysis results indicated that the bacterial community in medium-high-temperature Daqu exhibited higher stability. Functional prediction results showed that medium-high temperature Daqu had significantly higher average relative abundance in three functions: carbohydrate transport and metabolism, cell wall/membrane biosynthesis, and cellular motility and signal transduction mechanisms, compared to high- temperature Daqu. A total of 114 strains, belonging to 9 genera and 17 species, were isolated and identified from the two types of Daqu samples, mainly Bacillus licheniformis. |
| format | Article |
| id | doaj-art-e60f8d45e1f34f749aa8f5aa25154603 |
| institution | Kabale University |
| issn | 0254-5071 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-e60f8d45e1f34f749aa8f5aa251546032025-08-20T03:29:56ZengEditorial Department of China BrewingZhongguo niangzao0254-50712025-05-0144514615310.11882/j.issn.0254-5071.2025.05.022Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniquesWANG Wenhang, CAI Wenchao, YU Peirong, CHEN Wei, SHAN Chunhui, GUO Zhuang, WANG Yurong01. Brewing Technology Industrial College, Hubei University of Arts and Sciences, Xiangyang 441053, China; ;2. Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China; ;3. Food College, Shihezi University, Shihezi 832000, China; ;4. Xinjiang Wuwu Liquor Industry Co., Ltd., Huyanghe 830099, ChinaTo investigate the differences in bacterial community structure between high- and medium-high-temperature Daqu, the bacterial community structure of the two types of Daqu was analyzed by third-generation sequencing technology, the correlation between bacterial community and gene functions of bacterial community were analyzed, and the strains were isolated and identified by microbial pure culture and molecular biology techniques. The results showed that the dominant bacterial phyla (average relative abundance>1%) in two types of Daqu included Firmicutes, and the dominant bacterial genera included Kroppenstedtia, and the dominant bacterial species included Kroppenstedtia eburnea. Principal coordinates analysis (PCoA) and linear discriminant analysis effect size (LEfSe) analysis results showed that there were significant differences in the bacterial community structure between the two types of Daqu, with Thermoactinomycetaceae and Dietziaceae as biomarkers for high- and medium-high-temperature Daqu, respectively. Correlation analysis results indicated that the bacterial community in medium-high-temperature Daqu exhibited higher stability. Functional prediction results showed that medium-high temperature Daqu had significantly higher average relative abundance in three functions: carbohydrate transport and metabolism, cell wall/membrane biosynthesis, and cellular motility and signal transduction mechanisms, compared to high- temperature Daqu. A total of 114 strains, belonging to 9 genera and 17 species, were isolated and identified from the two types of Daqu samples, mainly Bacillus licheniformis.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-5-146.pdfhigh-temperature daqu|medium-high-temperature daqu|pacbio smrt sequencing|bacterial flora|isolation and identification |
| spellingShingle | WANG Wenhang, CAI Wenchao, YU Peirong, CHEN Wei, SHAN Chunhui, GUO Zhuang, WANG Yurong Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques Zhongguo niangzao high-temperature daqu|medium-high-temperature daqu|pacbio smrt sequencing|bacterial flora|isolation and identification |
| title | Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques |
| title_full | Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques |
| title_fullStr | Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques |
| title_full_unstemmed | Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques |
| title_short | Analysis of bacterial community structure in different types of Daqu based on third-generation sequencing and pure culture techniques |
| title_sort | analysis of bacterial community structure in different types of daqu based on third generation sequencing and pure culture techniques |
| topic | high-temperature daqu|medium-high-temperature daqu|pacbio smrt sequencing|bacterial flora|isolation and identification |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-5-146.pdf |
| work_keys_str_mv | AT wangwenhangcaiwenchaoyupeirongchenweishanchunhuiguozhuangwangyurong analysisofbacterialcommunitystructureindifferenttypesofdaqubasedonthirdgenerationsequencingandpureculturetechniques |