Comparison of genotyping assays for detection of targeted CRISPR/Cas mutagenesis in highly polyploid sugarcane

Comparison of genotyping assays for detection of targeted CRISPR/Cas mutagenesis in highly polyploid sugarcane

Sugarcane (Saccharum spp.) is an important biofuel feedstock and a leading source of global table sugar. Saccharum hybrid cultivars are highly polyploid (2n = 100–130), containing large numbers of functionally redundant hom(e)ologs in their genomes. Genome editing with sequence-specific nucleases ho...

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Bibliographic Details
Main Authors: Eleanor J. Brant, David May, Ayman Eid, Fredy Altpeter
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Genome Editing
Subjects:
polyploid
genotyping
mutations
CRISPR/Cas9
high resolution melt
capillary electrophoresis
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2024.1505844/full
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