Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective

Abstract Antimicrobial resistance (AMR) is a growing concern in veterinary and human medicine, impacting treatment efficacy and public health. This 18-month longitudinal study monitored AMR trends in bovine mastitis pathogens in the Banaskantha district of North Gujarat, India. A total of 424 bacter...

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Main Authors: Janki Panchal, Sandip Patel, Arun Patel, Harshad Chauhan, Kishan Sharma, Milan Dabhi, Mehul Shrimali, Sushil Mohapatra, Aakash Thakore, Jignesh Prajapati, Dweipayan Goswami
Format: Article
Language:English
Published: Springer 2025-04-01
Series:Discover Applied Sciences
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Online Access:https://doi.org/10.1007/s42452-025-06977-4
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author Janki Panchal
Sandip Patel
Arun Patel
Harshad Chauhan
Kishan Sharma
Milan Dabhi
Mehul Shrimali
Sushil Mohapatra
Aakash Thakore
Jignesh Prajapati
Dweipayan Goswami
author_facet Janki Panchal
Sandip Patel
Arun Patel
Harshad Chauhan
Kishan Sharma
Milan Dabhi
Mehul Shrimali
Sushil Mohapatra
Aakash Thakore
Jignesh Prajapati
Dweipayan Goswami
author_sort Janki Panchal
collection DOAJ
description Abstract Antimicrobial resistance (AMR) is a growing concern in veterinary and human medicine, impacting treatment efficacy and public health. This 18-month longitudinal study monitored AMR trends in bovine mastitis pathogens in the Banaskantha district of North Gujarat, India. A total of 424 bacterial isolates were analyzed following Clinical and Laboratory Standards Institute (CLSI) guidelines, with bacterial identification using MALDI-TOF mass spectrometry, and data analysed through the World Health Organization (WHO) WHONET software. Escherichia coli and Staphylococcus aureus were the most prevalent pathogens, exhibiting high resistance to cefotaxime/clavulanic acid and penicillin G, respectively. The study identified multidrug-resistant (MDR) and pan-drug-resistant (PDR) strains, raising significant treatment concerns. These findings emphasize the urgent need for ongoing AMR surveillance, responsible antibiotic use, and stricter regulations to mitigate resistance risks. A One Health approach is essential to address the interconnected threats posed by AMR across human, animal, and environmental health.
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record_format Article
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spelling doaj-art-e5fcd43ae36342eca0d10b057e2da8642025-08-20T02:10:49ZengSpringerDiscover Applied Sciences3004-92612025-04-017511810.1007/s42452-025-06977-4Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspectiveJanki Panchal0Sandip Patel1Arun Patel2Harshad Chauhan3Kishan Sharma4Milan Dabhi5Mehul Shrimali6Sushil Mohapatra7Aakash Thakore8Jignesh Prajapati9Dweipayan Goswami10Department of Microbiology & Biotechnology, University School of Sciences, Gujarat UniversityDepartment of Veterinary Microbiology and Animal Biotechnology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Veterinary Microbiology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Veterinary Microbiology and Animal Biotechnology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Veterinary Microbiology and Animal Biotechnology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Microbiology & Biotechnology, University School of Sciences, Gujarat UniversityDepartment of Veterinary Microbiology and Animal Biotechnology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Veterinary Microbiology and Animal Biotechnology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Veterinary Microbiology and Animal Biotechnology, College of Veterinary Sciences & Animal Husbandry, Kamdhenu UniversityDepartment of Biochemistry and Forensic Science, School of Sciences, Gujarat UniversityDepartment of Microbiology & Biotechnology, University School of Sciences, Gujarat UniversityAbstract Antimicrobial resistance (AMR) is a growing concern in veterinary and human medicine, impacting treatment efficacy and public health. This 18-month longitudinal study monitored AMR trends in bovine mastitis pathogens in the Banaskantha district of North Gujarat, India. A total of 424 bacterial isolates were analyzed following Clinical and Laboratory Standards Institute (CLSI) guidelines, with bacterial identification using MALDI-TOF mass spectrometry, and data analysed through the World Health Organization (WHO) WHONET software. Escherichia coli and Staphylococcus aureus were the most prevalent pathogens, exhibiting high resistance to cefotaxime/clavulanic acid and penicillin G, respectively. The study identified multidrug-resistant (MDR) and pan-drug-resistant (PDR) strains, raising significant treatment concerns. These findings emphasize the urgent need for ongoing AMR surveillance, responsible antibiotic use, and stricter regulations to mitigate resistance risks. A One Health approach is essential to address the interconnected threats posed by AMR across human, animal, and environmental health.https://doi.org/10.1007/s42452-025-06977-4Antimicrobial resistanceBovine mastitisWHONET.Drug-resistant pathogensOne health
spellingShingle Janki Panchal
Sandip Patel
Arun Patel
Harshad Chauhan
Kishan Sharma
Milan Dabhi
Mehul Shrimali
Sushil Mohapatra
Aakash Thakore
Jignesh Prajapati
Dweipayan Goswami
Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective
Discover Applied Sciences
Antimicrobial resistance
Bovine mastitis
WHONET.
Drug-resistant pathogens
One health
title Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective
title_full Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective
title_fullStr Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective
title_full_unstemmed Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective
title_short Longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in North Gujarat: an 18-month perspective
title_sort longitudinal surveillance and analysis of antimicrobial resistance in bovine pathogens in north gujarat an 18 month perspective
topic Antimicrobial resistance
Bovine mastitis
WHONET.
Drug-resistant pathogens
One health
url https://doi.org/10.1007/s42452-025-06977-4
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