The analysis of apricot and sweet cherry pollen viability after cryopreservation
Cryopreservation minimizes the risk of losing valuable accessions and reduces the material costs of plant resources maintenance. By using the method of pollen long-term storage in cryobanks, it is possible to keep pollen grains viable for an unlimited time. In the course of the research, cryoconserv...
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| Format: | Article |
| Language: | English |
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2019-01-01
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| Series: | Труды по прикладной ботанике, генетике и селекции |
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| Online Access: | https://elpub.vir.nw.ru/jour/article/view/289 |
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| author | M. N. Sitnikov V. G. Verzhuk A. V. Pavlov D. D. Bondaruk |
| author_facet | M. N. Sitnikov V. G. Verzhuk A. V. Pavlov D. D. Bondaruk |
| author_sort | M. N. Sitnikov |
| collection | DOAJ |
| description | Cryopreservation minimizes the risk of losing valuable accessions and reduces the material costs of plant resources maintenance. By using the method of pollen long-term storage in cryobanks, it is possible to keep pollen grains viable for an unlimited time. In the course of the research, cryoconservation of pollen of stone fruit crops was performed and pollen viability was checked after exposure to ultra-low temperatures (-196°С) under laboratory conditions. The studies were carried out on apricot (Armeniaca vulgaris Lam.) and sweet cherry (Cerasus avium (L.) Moench) plants, from which pollen was collected. Evaluation of viability in laboratory conditions was carried out by germination on various nutrient media. The possibility of long-term pollen preservation in liquid nitrogen vapor is shown. Based on the results obtained, a collection of pollen is being created for implementing various breeding programs. |
| format | Article |
| id | doaj-art-e5f70c8d5ebc4276802f7575537143d9 |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-e5f70c8d5ebc4276802f7575537143d92025-02-03T08:31:11ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822019-01-01179315215810.30901/2227-8834-2018-3-152-158287The analysis of apricot and sweet cherry pollen viability after cryopreservationM. N. Sitnikov0V. G. Verzhuk1A. V. Pavlov2D. D. Bondaruk3N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesCryopreservation minimizes the risk of losing valuable accessions and reduces the material costs of plant resources maintenance. By using the method of pollen long-term storage in cryobanks, it is possible to keep pollen grains viable for an unlimited time. In the course of the research, cryoconservation of pollen of stone fruit crops was performed and pollen viability was checked after exposure to ultra-low temperatures (-196°С) under laboratory conditions. The studies were carried out on apricot (Armeniaca vulgaris Lam.) and sweet cherry (Cerasus avium (L.) Moench) plants, from which pollen was collected. Evaluation of viability in laboratory conditions was carried out by germination on various nutrient media. The possibility of long-term pollen preservation in liquid nitrogen vapor is shown. Based on the results obtained, a collection of pollen is being created for implementing various breeding programs.https://elpub.vir.nw.ru/jour/article/view/289cryopreservationultra-low temperaturesapricotsweet cherrypollenbiodiversity conservation |
| spellingShingle | M. N. Sitnikov V. G. Verzhuk A. V. Pavlov D. D. Bondaruk The analysis of apricot and sweet cherry pollen viability after cryopreservation Труды по прикладной ботанике, генетике и селекции cryopreservation ultra-low temperatures apricot sweet cherry pollen biodiversity conservation |
| title | The analysis of apricot and sweet cherry pollen viability after cryopreservation |
| title_full | The analysis of apricot and sweet cherry pollen viability after cryopreservation |
| title_fullStr | The analysis of apricot and sweet cherry pollen viability after cryopreservation |
| title_full_unstemmed | The analysis of apricot and sweet cherry pollen viability after cryopreservation |
| title_short | The analysis of apricot and sweet cherry pollen viability after cryopreservation |
| title_sort | analysis of apricot and sweet cherry pollen viability after cryopreservation |
| topic | cryopreservation ultra-low temperatures apricot sweet cherry pollen biodiversity conservation |
| url | https://elpub.vir.nw.ru/jour/article/view/289 |
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