Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple
Myo-inositol and its derivatives play important roles in the tolerance of higher plants to abiotic stresses, and myo-inositol-1-phosphate synthase (MIPS) is the rate-limiting enzyme in myo-inositol biosynthesis. In this study, we found that increased myo-inositol biosynthesis enhanced drought tolera...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921638224 |
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| author | Ling-yu HU Hong YUE Jing-yun ZHANG Yang-tian-su LI Xiao-qing GONG Kun ZHOU Feng-wang MA |
| author_facet | Ling-yu HU Hong YUE Jing-yun ZHANG Yang-tian-su LI Xiao-qing GONG Kun ZHOU Feng-wang MA |
| author_sort | Ling-yu HU |
| collection | DOAJ |
| description | Myo-inositol and its derivatives play important roles in the tolerance of higher plants to abiotic stresses, and myo-inositol-1-phosphate synthase (MIPS) is the rate-limiting enzyme in myo-inositol biosynthesis. In this study, we found that increased myo-inositol biosynthesis enhanced drought tolerance in MdMIPS1-overexpressing apple lines under short-term progressive drought stress. The effect of myo-inositol appeared to be mediated by the increased accumulation of osmoprotectants such as glucose, sucrose, and proline, and by the increased activities of antioxidant enzymes that eliminate reactive oxygen species. Moreover, enhanced water-use efficiency (WUE) was observed in MdMIPS1-overexpressing apple lines under long-term moderate water deficit conditions that mimicked the water availability in the soil of the Loess Plateau. Enhanced WUE may have been associated with the synergistic regulation of osmotic balance and stomatal aperture mediated by increased myo-inositol biosynthesis. Taken together, our findings shed light on the positive effects of MdMIPS1-mediated myo-inositol biosynthesis on drought tolerance and WUE in apple. |
| format | Article |
| id | doaj-art-e5ee31062caa4a4d868dec0e279e46ce |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2022-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e5ee31062caa4a4d868dec0e279e46ce2025-08-20T03:57:59ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192022-07-012171968198110.1016/S2095-3119(21)63822-4Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in appleLing-yu HU0Hong YUE1Jing-yun ZHANG2Yang-tian-su LI3Xiao-qing GONG4Kun ZHOU5Feng-wang MA6State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCorrespondence MA Feng-wang, Tel: +86-29-87082648; State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaMyo-inositol and its derivatives play important roles in the tolerance of higher plants to abiotic stresses, and myo-inositol-1-phosphate synthase (MIPS) is the rate-limiting enzyme in myo-inositol biosynthesis. In this study, we found that increased myo-inositol biosynthesis enhanced drought tolerance in MdMIPS1-overexpressing apple lines under short-term progressive drought stress. The effect of myo-inositol appeared to be mediated by the increased accumulation of osmoprotectants such as glucose, sucrose, and proline, and by the increased activities of antioxidant enzymes that eliminate reactive oxygen species. Moreover, enhanced water-use efficiency (WUE) was observed in MdMIPS1-overexpressing apple lines under long-term moderate water deficit conditions that mimicked the water availability in the soil of the Loess Plateau. Enhanced WUE may have been associated with the synergistic regulation of osmotic balance and stomatal aperture mediated by increased myo-inositol biosynthesis. Taken together, our findings shed light on the positive effects of MdMIPS1-mediated myo-inositol biosynthesis on drought tolerance and WUE in apple.http://www.sciencedirect.com/science/article/pii/S2095311921638224appleMdMIPS1myo-inositolwater deficitdrought tolerancewater-use efficiency |
| spellingShingle | Ling-yu HU Hong YUE Jing-yun ZHANG Yang-tian-su LI Xiao-qing GONG Kun ZHOU Feng-wang MA Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple Journal of Integrative Agriculture apple MdMIPS1 myo-inositol water deficit drought tolerance water-use efficiency |
| title | Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple |
| title_full | Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple |
| title_fullStr | Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple |
| title_full_unstemmed | Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple |
| title_short | Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple |
| title_sort | overexpression of mdmips1 enhances drought tolerance and water use efficiency in apple |
| topic | apple MdMIPS1 myo-inositol water deficit drought tolerance water-use efficiency |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921638224 |
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