Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis
Abstract Phoma stem canker disease of oilseed rape (Brassica napus) is caused by the extracellular fungal pathogen Leptosphaeria maculans. Although this pathogen resides exclusively in apoplastic spaces surrounding plant cells, the significance of extracellular vesicles (EVs) has not been assessed....
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| Format: | Article |
| Language: | English |
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Wiley
2025-02-01
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| Series: | Journal of Extracellular Biology |
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| Online Access: | https://doi.org/10.1002/jex2.70029 |
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| author | Nathaniel Hearfield Dominik Brotherton Zedi Gao Jameel Inal Henrik U. Stotz |
| author_facet | Nathaniel Hearfield Dominik Brotherton Zedi Gao Jameel Inal Henrik U. Stotz |
| author_sort | Nathaniel Hearfield |
| collection | DOAJ |
| description | Abstract Phoma stem canker disease of oilseed rape (Brassica napus) is caused by the extracellular fungal pathogen Leptosphaeria maculans. Although this pathogen resides exclusively in apoplastic spaces surrounding plant cells, the significance of extracellular vesicles (EVs) has not been assessed. Here, we show a method to collect apoplastic fluids (AFs) from infected leaves or cotyledons for collection of EVs during the process of host colonisation. The 15,000 × g supernatants of AFs were shown to contain ribulose‐bisphosphate carboxylase (RuBisCO) at 7 days post‐inoculation with L. maculans, a protein that was absent from unchallenged cotyledons. RuBisCO release coincided with the switch from biotrophy to necrotrophy, suggesting the involvement of host cell death. However, RuBisCO release did not differ between compatible and incompatible interactions, suggesting necrotrophic host cell death might not be the only process involved. EVs were also collected from axenic fungal cultures and characterised for their particle size distribution using nanoparticle tracking analysis and transmission electron microscopy. The protein composition of EV‐enriched fractions was analysed using SDS‐PAGE and proteomics. Enrichment analysis of gene ontology terms provided evidence for involvement of glucan and chitin metabolism as well as catalase and peptidase activities. Most of the proteins identified have previously been found in EV studies and/or EV databases, and for most of the proteins evidence was found for an involvement in pathogenicity/virulence. |
| format | Article |
| id | doaj-art-e5ea58d74ac74609987488dd5ee346de |
| institution | DOAJ |
| issn | 2768-2811 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Biology |
| spelling | doaj-art-e5ea58d74ac74609987488dd5ee346de2025-08-20T02:45:04ZengWileyJournal of Extracellular Biology2768-28112025-02-0142n/an/a10.1002/jex2.70029Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesisNathaniel Hearfield0Dominik Brotherton1Zedi Gao2Jameel Inal3Henrik U. Stotz4Centre for Agriculture, Food and Environmental Management University of Hertfordshire Hatfield UKSchool of Life & Medical Sciences University of Hertfordshire Hatfield UKSchool of Life & Medical Sciences University of Hertfordshire Hatfield UKSchool of Life & Medical Sciences University of Hertfordshire Hatfield UKCentre for Agriculture, Food and Environmental Management University of Hertfordshire Hatfield UKAbstract Phoma stem canker disease of oilseed rape (Brassica napus) is caused by the extracellular fungal pathogen Leptosphaeria maculans. Although this pathogen resides exclusively in apoplastic spaces surrounding plant cells, the significance of extracellular vesicles (EVs) has not been assessed. Here, we show a method to collect apoplastic fluids (AFs) from infected leaves or cotyledons for collection of EVs during the process of host colonisation. The 15,000 × g supernatants of AFs were shown to contain ribulose‐bisphosphate carboxylase (RuBisCO) at 7 days post‐inoculation with L. maculans, a protein that was absent from unchallenged cotyledons. RuBisCO release coincided with the switch from biotrophy to necrotrophy, suggesting the involvement of host cell death. However, RuBisCO release did not differ between compatible and incompatible interactions, suggesting necrotrophic host cell death might not be the only process involved. EVs were also collected from axenic fungal cultures and characterised for their particle size distribution using nanoparticle tracking analysis and transmission electron microscopy. The protein composition of EV‐enriched fractions was analysed using SDS‐PAGE and proteomics. Enrichment analysis of gene ontology terms provided evidence for involvement of glucan and chitin metabolism as well as catalase and peptidase activities. Most of the proteins identified have previously been found in EV studies and/or EV databases, and for most of the proteins evidence was found for an involvement in pathogenicity/virulence.https://doi.org/10.1002/jex2.70029apoplastextracellular vesiclesnanoscalepectinphytopathogenprotein network |
| spellingShingle | Nathaniel Hearfield Dominik Brotherton Zedi Gao Jameel Inal Henrik U. Stotz Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis Journal of Extracellular Biology apoplast extracellular vesicles nanoscale pectin phytopathogen protein network |
| title | Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis |
| title_full | Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis |
| title_fullStr | Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis |
| title_full_unstemmed | Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis |
| title_short | Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis |
| title_sort | establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis |
| topic | apoplast extracellular vesicles nanoscale pectin phytopathogen protein network |
| url | https://doi.org/10.1002/jex2.70029 |
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