Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway
Epithelial ovarian cancer (EOC) is a highly aggressive malignancy with a poor prognosis due to late-stage diagnosis and the lack of reliable biomarkers for early detection. Exosomes, small vesicles involved in intercellular communication, play a critical role in cancer progression by promoting migra...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2025-03-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2025024 |
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| author | Yin Zeyuan Ma Jiachen Adu-Amankwaah Joseph Xie Guangyan Wang Yinghao Tai Wei Sun Zhenquan Huang Chuting Chen Guanfeng Fu Tong Zhang Bei Zhou Xueyan |
| author_facet | Yin Zeyuan Ma Jiachen Adu-Amankwaah Joseph Xie Guangyan Wang Yinghao Tai Wei Sun Zhenquan Huang Chuting Chen Guanfeng Fu Tong Zhang Bei Zhou Xueyan |
| author_sort | Yin Zeyuan |
| collection | DOAJ |
| description | Epithelial ovarian cancer (EOC) is a highly aggressive malignancy with a poor prognosis due to late-stage diagnosis and the lack of reliable biomarkers for early detection. Exosomes, small vesicles involved in intercellular communication, play a critical role in cancer progression by promoting migration, proliferation, and metastasis. This study investigates the role of exosomal proteins in EOC cell migration and identifies potential biomarkers. Exosomes are isolated from the ascites fluid of EOC patients (C-Exos) and benign ovarian disease patients (B-Exos), and mass spectrometry analysis of clinical samples reveals 185 differentially expressed proteins, with integrin alpha 3 (ITGA3) being strongly associated with poor prognosis. ITGA3 is transported via exosomes to recipient EOC cells, where it is released into the cytoplasm and translocated to the cell membrane. This localization enables ITGA3 to activate the intracellular signaling pathways that drive EOC migration. Immunoprecipitation mass spectrometry of clinical samples reveals that ITGA3 may influence EOC migration through the S100A7/p-ERK signaling pathway. Mechanistically, ITGA3 activates ERK signaling through S100A7, promoting cell migration. In vivo, exosomes enrich with ITGA3 facilitates tumor growth and migration, whereas ITGA3 knockdown reduces these effects. These findings suggest that exosomal ITGA3, via the S100A7/p-ERK signaling pathway, promotes EOC cell migration. ITGA3 could serve as a prognostic biomarker and therapeutic target in EOC. Targeting the ITGA3/S100A7 axis may help suppress migration, suggesting a promising strategy to improve EOC patient outcomes. |
| format | Article |
| id | doaj-art-e5e5baea0f014d13bd2cb54a8abe9326 |
| institution | OA Journals |
| issn | 1672-9145 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-e5e5baea0f014d13bd2cb54a8abe93262025-08-20T02:37:43ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452025-03-01571006101910.3724/abbs.202502420d259ccExosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathwayYin Zeyuan0Ma Jiachen1Adu-Amankwaah Joseph2Xie Guangyan3Wang Yinghao4Tai Wei5Sun Zhenquan6Huang Chuting7Chen Guanfeng8Fu Tong9Zhang Bei10Zhou Xueyan11["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China","The First Clinical Medical School, Xuzhou Medical University, Xuzhou 221004, China","Department of Cardiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, China","Cardiovascular Sciences, University of Manchester, Manchester M13 9NT, UK"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Department of Physiology, Xuzhou Medical University, Xuzhou 221004, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]["Department of Obstetrics and Gynecology, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou 221009, China"]["Department of Obstetrics and Gynecology, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou 221009, China"]["Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"]Epithelial ovarian cancer (EOC) is a highly aggressive malignancy with a poor prognosis due to late-stage diagnosis and the lack of reliable biomarkers for early detection. Exosomes, small vesicles involved in intercellular communication, play a critical role in cancer progression by promoting migration, proliferation, and metastasis. This study investigates the role of exosomal proteins in EOC cell migration and identifies potential biomarkers. Exosomes are isolated from the ascites fluid of EOC patients (C-Exos) and benign ovarian disease patients (B-Exos), and mass spectrometry analysis of clinical samples reveals 185 differentially expressed proteins, with integrin alpha 3 (ITGA3) being strongly associated with poor prognosis. ITGA3 is transported via exosomes to recipient EOC cells, where it is released into the cytoplasm and translocated to the cell membrane. This localization enables ITGA3 to activate the intracellular signaling pathways that drive EOC migration. Immunoprecipitation mass spectrometry of clinical samples reveals that ITGA3 may influence EOC migration through the S100A7/p-ERK signaling pathway. Mechanistically, ITGA3 activates ERK signaling through S100A7, promoting cell migration. In vivo, exosomes enrich with ITGA3 facilitates tumor growth and migration, whereas ITGA3 knockdown reduces these effects. These findings suggest that exosomal ITGA3, via the S100A7/p-ERK signaling pathway, promotes EOC cell migration. ITGA3 could serve as a prognostic biomarker and therapeutic target in EOC. Targeting the ITGA3/S100A7 axis may help suppress migration, suggesting a promising strategy to improve EOC patient outcomes.https://www.sciengine.com/doi/10.3724/abbs.2025024human ovarian cancerexosomeintegrin alpha 3 (ITGA3)S100A7cell migration |
| spellingShingle | Yin Zeyuan Ma Jiachen Adu-Amankwaah Joseph Xie Guangyan Wang Yinghao Tai Wei Sun Zhenquan Huang Chuting Chen Guanfeng Fu Tong Zhang Bei Zhou Xueyan Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway Acta Biochimica et Biophysica Sinica human ovarian cancer exosome integrin alpha 3 (ITGA3) S100A7 cell migration |
| title | Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway |
| title_full | Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway |
| title_fullStr | Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway |
| title_full_unstemmed | Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway |
| title_short | Exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the S100A7/p-ERK signaling pathway |
| title_sort | exosomal integrin alpha 3 promotes epithelial ovarian cancer cell migration via the s100a7 p erk signaling pathway |
| topic | human ovarian cancer exosome integrin alpha 3 (ITGA3) S100A7 cell migration |
| url | https://www.sciengine.com/doi/10.3724/abbs.2025024 |
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