Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i>
Interferon-gamma release assays (IGRAs) have gained attention for the diagnosis of latent tuberculosis infection (LTBI) due to their higher specificity compared to the tuberculin skin test (TST). However, the IGRA’s performance varies across different populations. This study evaluated the diagnostic...
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MDPI AG
2024-09-01
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| Online Access: | https://www.mdpi.com/2075-4418/14/19/2130 |
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| author | Richard Kutame Gifty Boateng Yaw Adusi-Poku Felix Sorvor Lorreta Antwi Florence Agyemang-Bioh Bright Ayensu Vincent Gyau-Boateng Franklin Asiedu-Bekoe |
| author_facet | Richard Kutame Gifty Boateng Yaw Adusi-Poku Felix Sorvor Lorreta Antwi Florence Agyemang-Bioh Bright Ayensu Vincent Gyau-Boateng Franklin Asiedu-Bekoe |
| author_sort | Richard Kutame |
| collection | DOAJ |
| description | Interferon-gamma release assays (IGRAs) have gained attention for the diagnosis of latent tuberculosis infection (LTBI) due to their higher specificity compared to the tuberculin skin test (TST). However, the IGRA’s performance varies across different populations. This study evaluated the diagnostic performance of three IGRAs (TBF-FIA, TBF-ELISA, and QFT-Plus) in Ghana, comparing them among individuals exposed and unexposed to MTB infection. Conducted in TB clinics across three regions, this prospective and cross-sectional study included healthy individuals with no known TB exposure (unexposed group) and patients with confirmed active TB (exposed group). Blood samples were tested using all three assays as per the manufacturers’ guidelines. The TBF-ELISA showed 3.4% higher sensitivity but 4.6% lower specificity compared to QFT-Plus. The TBF-FIA had sensitivity of 78.5–87.3% and specificity of 82.9–90.0%. These findings indicate that while the three IGRAs offer similar diagnostic accuracy, the variations in specificity and limited data on assays like TBF-FIA require further investigation. |
| format | Article |
| id | doaj-art-e5daba59e9e948f7a55fa912088209f7 |
| institution | OA Journals |
| issn | 2075-4418 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj-art-e5daba59e9e948f7a55fa912088209f72025-08-20T01:47:44ZengMDPI AGDiagnostics2075-44182024-09-011419213010.3390/diagnostics14192130Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i>Richard Kutame0Gifty Boateng1Yaw Adusi-Poku2Felix Sorvor3Lorreta Antwi4Florence Agyemang-Bioh5Bright Ayensu6Vincent Gyau-Boateng7Franklin Asiedu-Bekoe8National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, GhanaNational Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, GhanaNational TB Control Programme, Korle Bu Teaching Hospital, Accra P.O. Box KB493, GhanaNational TB Control Programme, Korle Bu Teaching Hospital, Accra P.O. Box KB493, GhanaNational Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, GhanaNational Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, GhanaNational Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, GhanaNational Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, GhanaPublic Health Division, Ghana Health Service, Korle Bu Teaching Hospital, P.M.B. Ministries, Accra P.O. Box MB582, GhanaInterferon-gamma release assays (IGRAs) have gained attention for the diagnosis of latent tuberculosis infection (LTBI) due to their higher specificity compared to the tuberculin skin test (TST). However, the IGRA’s performance varies across different populations. This study evaluated the diagnostic performance of three IGRAs (TBF-FIA, TBF-ELISA, and QFT-Plus) in Ghana, comparing them among individuals exposed and unexposed to MTB infection. Conducted in TB clinics across three regions, this prospective and cross-sectional study included healthy individuals with no known TB exposure (unexposed group) and patients with confirmed active TB (exposed group). Blood samples were tested using all three assays as per the manufacturers’ guidelines. The TBF-ELISA showed 3.4% higher sensitivity but 4.6% lower specificity compared to QFT-Plus. The TBF-FIA had sensitivity of 78.5–87.3% and specificity of 82.9–90.0%. These findings indicate that while the three IGRAs offer similar diagnostic accuracy, the variations in specificity and limited data on assays like TBF-FIA require further investigation.https://www.mdpi.com/2075-4418/14/19/2130IGRAinterferon gammaevaluationtuberculosisdiagnosis<i>Mycobacterium tuberculosis</i> |
| spellingShingle | Richard Kutame Gifty Boateng Yaw Adusi-Poku Felix Sorvor Lorreta Antwi Florence Agyemang-Bioh Bright Ayensu Vincent Gyau-Boateng Franklin Asiedu-Bekoe Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i> Diagnostics IGRA interferon gamma evaluation tuberculosis diagnosis <i>Mycobacterium tuberculosis</i> |
| title | Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i> |
| title_full | Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i> |
| title_fullStr | Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i> |
| title_full_unstemmed | Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i> |
| title_short | Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for <i>Mycobacterium tuberculosis</i> |
| title_sort | evaluation of diagnostic performance of three commercial interferon gamma release assays for i mycobacterium tuberculosis i |
| topic | IGRA interferon gamma evaluation tuberculosis diagnosis <i>Mycobacterium tuberculosis</i> |
| url | https://www.mdpi.com/2075-4418/14/19/2130 |
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