A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats
Chronic ingestion of saturated fatty acids (SFAs) was previously shown to compromise blood-brain barrier integrity, leading to brain parenchymal extravasation of apolipoprotein B (apo B) lipoproteins enriched in amyloid beta. In contrast, diets enriched in mono- or polyunsaturated (PUFA) oils had no...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/647689 |
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| author | Menuka M. Pallebage-Gamarallage Virginie Lam Ryusuke Takechi Susan Galloway John C. L. Mamo |
| author_facet | Menuka M. Pallebage-Gamarallage Virginie Lam Ryusuke Takechi Susan Galloway John C. L. Mamo |
| author_sort | Menuka M. Pallebage-Gamarallage |
| collection | DOAJ |
| description | Chronic ingestion of saturated fatty acids (SFAs) was previously shown to compromise blood-brain barrier integrity, leading to brain parenchymal extravasation of apolipoprotein B (apo B) lipoproteins enriched in amyloid beta. In contrast, diets enriched in mono- or polyunsaturated (PUFA) oils had no detrimental effect. Rather, n3 and n6 oils generally confer protection via suppression of inflammation. This study investigated in wild-type mice if a PUFA diet enriched in docosahexanoic acid (DHA) restored blood-brain barrier integrity and attenuated parenchymal apo B abundance induced by chronic ingestion of SFA. Cerebrovascular leakage of apo B was quantitated utilising immunofluorescent staining. The plasma concentration of brain-derived S100𝛽 was measured as a marker of cerebrovascular inflammation. In mice fed SFA for 3 months, provision thereafter of a DHA-enriched diet exacerbated parenchymal apo B retention, concomitant with a significant increase in plasma cholesterol. In contrast, provision of a low-fat diet following chronic SFA feeding had no effect on SFA-induced parenchymal apo B. The findings suggest that in a heightened state of cerebrovascular inflammation, the provision of unsaturated fatty acids may be detrimental, possibly as a consequence of a greater susceptibility for oxidation. |
| format | Article |
| id | doaj-art-e5cb17a3758045a4a1e0f2fcd2830c2d |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-e5cb17a3758045a4a1e0f2fcd2830c2d2025-02-03T05:52:22ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322012-01-01201210.1155/2012/647689647689A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated FatsMenuka M. Pallebage-Gamarallage0Virginie Lam1Ryusuke Takechi2Susan Galloway3John C. L. Mamo4Curtin Health Innovation Research Institute, Curtin University of Technology, Perth, Western Australia 6845, AustraliaCurtin Health Innovation Research Institute, Curtin University of Technology, Perth, Western Australia 6845, AustraliaCurtin Health Innovation Research Institute, Curtin University of Technology, Perth, Western Australia 6845, AustraliaCurtin Health Innovation Research Institute, Curtin University of Technology, Perth, Western Australia 6845, AustraliaCurtin Health Innovation Research Institute, Curtin University of Technology, Perth, Western Australia 6845, AustraliaChronic ingestion of saturated fatty acids (SFAs) was previously shown to compromise blood-brain barrier integrity, leading to brain parenchymal extravasation of apolipoprotein B (apo B) lipoproteins enriched in amyloid beta. In contrast, diets enriched in mono- or polyunsaturated (PUFA) oils had no detrimental effect. Rather, n3 and n6 oils generally confer protection via suppression of inflammation. This study investigated in wild-type mice if a PUFA diet enriched in docosahexanoic acid (DHA) restored blood-brain barrier integrity and attenuated parenchymal apo B abundance induced by chronic ingestion of SFA. Cerebrovascular leakage of apo B was quantitated utilising immunofluorescent staining. The plasma concentration of brain-derived S100𝛽 was measured as a marker of cerebrovascular inflammation. In mice fed SFA for 3 months, provision thereafter of a DHA-enriched diet exacerbated parenchymal apo B retention, concomitant with a significant increase in plasma cholesterol. In contrast, provision of a low-fat diet following chronic SFA feeding had no effect on SFA-induced parenchymal apo B. The findings suggest that in a heightened state of cerebrovascular inflammation, the provision of unsaturated fatty acids may be detrimental, possibly as a consequence of a greater susceptibility for oxidation.http://dx.doi.org/10.1155/2012/647689 |
| spellingShingle | Menuka M. Pallebage-Gamarallage Virginie Lam Ryusuke Takechi Susan Galloway John C. L. Mamo A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats International Journal of Vascular Medicine |
| title | A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats |
| title_full | A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats |
| title_fullStr | A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats |
| title_full_unstemmed | A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats |
| title_short | A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats |
| title_sort | diet enriched in docosahexanoic acid exacerbates brain parenchymal extravasation of apo b lipoproteins induced by chronic ingestion of saturated fats |
| url | http://dx.doi.org/10.1155/2012/647689 |
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