Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis
We analyzed the effect of alliin on the recovery of mouse testicular function and structure following estradiol treatment as well as on apoptosis regulation. During the cultivation of testicular cells, high-concentration estradiol suppressed Casp-3; PCNA, mTOR, and PI3K signaling increased; and cell...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/46/11/776 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850267226027851776 |
|---|---|
| author | Dae-Seung Kim Min-Jee Oh Sang-Hwan Kim |
| author_facet | Dae-Seung Kim Min-Jee Oh Sang-Hwan Kim |
| author_sort | Dae-Seung Kim |
| collection | DOAJ |
| description | We analyzed the effect of alliin on the recovery of mouse testicular function and structure following estradiol treatment as well as on apoptosis regulation. During the cultivation of testicular cells, high-concentration estradiol suppressed Casp-3; PCNA, mTOR, and PI3K signaling increased; and cell proliferation in the testes was abnormally increased. Therefore, estradiol treatment increased the proportion of abnormal cells. The estradiol and 2.5 μM of alliin treatment increased Casp-3 levels and suppressed Bcl-2, PCNA, mTOR, and PI3K expression. Additionally, treatment with estradiol caused tissue loss. Furthermore, Ca<sup>2+</sup> deposition decreased, TNF-r protein expression increased, and the levels of other protein markers of cell survival and death decreased. Tissue recovery and restoration of the testes occurred after alliin treatment; the gene expression of cell survival and death markers, except for TNF-r, increased with increasing Ca<sup>2+</sup> deposition. Cell proliferation and tissue reorganization may correlate with an increased signal of intrinsic apoptosis owing to increased Ca<sup>2+</sup> deposition. Therefore, treatment with alliin may regulate the apoptosis of cells with normal or abnormal signal transduction and help to revert testicular dysfunction. |
| format | Article |
| id | doaj-art-e5c9ed0d6517489e8d053ad89e43e5ad |
| institution | OA Journals |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-e5c9ed0d6517489e8d053ad89e43e5ad2025-08-20T01:53:53ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014611130211303410.3390/cimb46110776Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating ApoptosisDae-Seung Kim0Min-Jee Oh1Sang-Hwan Kim2Institute of Applied Humanimal Science, Hankyong National University, 327, Jungang-ro, Unsung 17579, Gyeonggi-do, Republic of KoreaGeneral Graduate School of Animal Life Convergence Science, Hankyong National University, 327, Jungang-ro, Ansung 17579, Gyeonggi-do, Republic of KoreaInstitute of Applied Humanimal Science, Hankyong National University, 327, Jungang-ro, Unsung 17579, Gyeonggi-do, Republic of KoreaWe analyzed the effect of alliin on the recovery of mouse testicular function and structure following estradiol treatment as well as on apoptosis regulation. During the cultivation of testicular cells, high-concentration estradiol suppressed Casp-3; PCNA, mTOR, and PI3K signaling increased; and cell proliferation in the testes was abnormally increased. Therefore, estradiol treatment increased the proportion of abnormal cells. The estradiol and 2.5 μM of alliin treatment increased Casp-3 levels and suppressed Bcl-2, PCNA, mTOR, and PI3K expression. Additionally, treatment with estradiol caused tissue loss. Furthermore, Ca<sup>2+</sup> deposition decreased, TNF-r protein expression increased, and the levels of other protein markers of cell survival and death decreased. Tissue recovery and restoration of the testes occurred after alliin treatment; the gene expression of cell survival and death markers, except for TNF-r, increased with increasing Ca<sup>2+</sup> deposition. Cell proliferation and tissue reorganization may correlate with an increased signal of intrinsic apoptosis owing to increased Ca<sup>2+</sup> deposition. Therefore, treatment with alliin may regulate the apoptosis of cells with normal or abnormal signal transduction and help to revert testicular dysfunction.https://www.mdpi.com/1467-3045/46/11/776alliintestesestrogenapoptosisendocrine disruption |
| spellingShingle | Dae-Seung Kim Min-Jee Oh Sang-Hwan Kim Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis Current Issues in Molecular Biology alliin testes estrogen apoptosis endocrine disruption |
| title | Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis |
| title_full | Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis |
| title_fullStr | Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis |
| title_full_unstemmed | Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis |
| title_short | Alliin Induces Reconstitution of Testes Damaged by Estrogen Overstimulation by Regulating Apoptosis |
| title_sort | alliin induces reconstitution of testes damaged by estrogen overstimulation by regulating apoptosis |
| topic | alliin testes estrogen apoptosis endocrine disruption |
| url | https://www.mdpi.com/1467-3045/46/11/776 |
| work_keys_str_mv | AT daeseungkim alliininducesreconstitutionoftestesdamagedbyestrogenoverstimulationbyregulatingapoptosis AT minjeeoh alliininducesreconstitutionoftestesdamagedbyestrogenoverstimulationbyregulatingapoptosis AT sanghwankim alliininducesreconstitutionoftestesdamagedbyestrogenoverstimulationbyregulatingapoptosis |