Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria
Introduction The pathogenic Escherichia coli bacterium that cause diseases within the intestine is called diarrheagenic E. coli (DEC) and is responsible for E. coli gastroenteritis. The present study aimed to identify the occurrence and antibiotic resistance profile of STEC/ EHEC and EPEC from water...
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European Publishing
2023-09-01
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| Series: | Public Health and Toxicology |
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| author | Musa Y. Tula Onaiwu I. Enabulele Endurance A. Ophori |
| author_facet | Musa Y. Tula Onaiwu I. Enabulele Endurance A. Ophori |
| author_sort | Musa Y. Tula |
| collection | DOAJ |
| description | Introduction
The pathogenic Escherichia coli
bacterium that cause diseases within the intestine is
called diarrheagenic E. coli (DEC) and is responsible for
E. coli gastroenteritis. The present study aimed to identify
the occurrence and antibiotic resistance profile of STEC/
EHEC and EPEC from water sources in the Adamawa-north
Senatorial zone, Nigeria.
Methods
Water samples from hand-dug wells and rivers/
streams from the study area were screened for the presence
of E. coli, and diarrheagenic E. coli (DEC) by polymerase chain
reactions. The antimicrobial susceptibility pattern of the DEC
pathotypes was determined by Kirby-Bauer method.
Results
Of the 256 water samples analyzed, 63 (24.6%)
E. coli were isolated. Of these, 44 (69.8%) and 19 (30.2%)
were isolated from river and well water sources, respectively.
Of the 63 E. coli isolates recovered from the water sources,
27 (42.9%) were non-sorbitol fermenting E. coli. Of these,
24 (88.9%) isolates were of STEC/EHEC pathotypes, 2
(7.4%) were of EPEC pathotype, while shiga toxins and
intimin genes were not amplified in 1 (3.7%) isolate. All
the STEC and EPEC exhibited complete resistance (100%)
to cotrimoxazole, ampicillin, nalidixic acid, augmentin, and
ceftriaxone. However, while 13 (54.2%) out of the 24 STEC
isolates demonstrated resistance to imipenem, all the EPEC
isolates remained susceptible to imipenem. The resistant
profile showed that the DEC pathotypes were resistant to
7–12 antibiotics with 8 (30.8%) of the 26 DEC resistant to
12 antibiotics.
Conclusions
The findings of this study contribute to a
better knowledge of the occurrence of STEC/EHEC and EPEC
in the major water sources of the study area and may serve
as reference point data for future use and epidemiological
surveillance. |
| format | Article |
| id | doaj-art-e5c176e93ba946ada28a675dd0c700ac |
| institution | DOAJ |
| issn | 2732-8929 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | European Publishing |
| record_format | Article |
| series | Public Health and Toxicology |
| spelling | doaj-art-e5c176e93ba946ada28a675dd0c700ac2025-08-20T02:49:47ZengEuropean PublishingPublic Health and Toxicology2732-89292023-09-01331810.18332/pht/172303172303Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, NigeriaMusa Y. Tula0https://orcid.org/0000-0001-8666-6839Onaiwu I. Enabulele1Endurance A. Ophori2Department of Biological Science Technology, Federal Polytechnic Mubi, Adamawa State, NigeriaDepartment of Microbiology, University of Benin, Benin-city, NigeriaDepartment of Microbiology, University of Benin, Benin-city, NigeriaIntroduction The pathogenic Escherichia coli bacterium that cause diseases within the intestine is called diarrheagenic E. coli (DEC) and is responsible for E. coli gastroenteritis. The present study aimed to identify the occurrence and antibiotic resistance profile of STEC/ EHEC and EPEC from water sources in the Adamawa-north Senatorial zone, Nigeria. Methods Water samples from hand-dug wells and rivers/ streams from the study area were screened for the presence of E. coli, and diarrheagenic E. coli (DEC) by polymerase chain reactions. The antimicrobial susceptibility pattern of the DEC pathotypes was determined by Kirby-Bauer method. Results Of the 256 water samples analyzed, 63 (24.6%) E. coli were isolated. Of these, 44 (69.8%) and 19 (30.2%) were isolated from river and well water sources, respectively. Of the 63 E. coli isolates recovered from the water sources, 27 (42.9%) were non-sorbitol fermenting E. coli. Of these, 24 (88.9%) isolates were of STEC/EHEC pathotypes, 2 (7.4%) were of EPEC pathotype, while shiga toxins and intimin genes were not amplified in 1 (3.7%) isolate. All the STEC and EPEC exhibited complete resistance (100%) to cotrimoxazole, ampicillin, nalidixic acid, augmentin, and ceftriaxone. However, while 13 (54.2%) out of the 24 STEC isolates demonstrated resistance to imipenem, all the EPEC isolates remained susceptible to imipenem. The resistant profile showed that the DEC pathotypes were resistant to 7–12 antibiotics with 8 (30.8%) of the 26 DEC resistant to 12 antibiotics. Conclusions The findings of this study contribute to a better knowledge of the occurrence of STEC/EHEC and EPEC in the major water sources of the study area and may serve as reference point data for future use and epidemiological surveillance.https://www.publichealthtoxicology.com/Occurrence-and-antibiotic-resistance-profile-of-shiga-toxin-producing-Escherichia,172303,0,2.htmlenteropathogenic e. colishiga toxin-producing e. coliwater sourcesmubidiarrheagenic |
| spellingShingle | Musa Y. Tula Onaiwu I. Enabulele Endurance A. Ophori Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria Public Health and Toxicology enteropathogenic e. coli shiga toxin-producing e. coli water sources mubi diarrheagenic |
| title | Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria |
| title_full | Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria |
| title_fullStr | Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria |
| title_full_unstemmed | Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria |
| title_short | Occurrence and antibiotic resistance profile of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from sources of water in Mubi Region, Adamawa State, Nigeria |
| title_sort | occurrence and antibiotic resistance profile of shiga toxin producing escherichia coli stec and enteropathogenic escherichia coli epec from sources of water in mubi region adamawa state nigeria |
| topic | enteropathogenic e. coli shiga toxin-producing e. coli water sources mubi diarrheagenic |
| url | https://www.publichealthtoxicology.com/Occurrence-and-antibiotic-resistance-profile-of-shiga-toxin-producing-Escherichia,172303,0,2.html |
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