Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.

<h4>Background</h4>HIV-infected infants in sub-Saharan Africa have rapid disease progression. We hypothesized that co-infection with cytomegalovirus (CMV) or Epstein Barr virus (EBV) increases mortality in HIV-infected infants.<h4>Methods</h4>257 antiretroviral therapy-naïve...

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Main Authors: Hlanai Gumbo, Bernard Chasekwa, James A Church, Robert Ntozini, Kuda Mutasa, Jean H Humphrey, Andrew J Prendergast
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0114870
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author Hlanai Gumbo
Bernard Chasekwa
James A Church
Robert Ntozini
Kuda Mutasa
Jean H Humphrey
Andrew J Prendergast
author_facet Hlanai Gumbo
Bernard Chasekwa
James A Church
Robert Ntozini
Kuda Mutasa
Jean H Humphrey
Andrew J Prendergast
author_sort Hlanai Gumbo
collection DOAJ
description <h4>Background</h4>HIV-infected infants in sub-Saharan Africa have rapid disease progression. We hypothesized that co-infection with cytomegalovirus (CMV) or Epstein Barr virus (EBV) increases mortality in HIV-infected infants.<h4>Methods</h4>257 antiretroviral therapy-naïve HIV-infected Zimbabwean infants were tested for CMV and EBV at 6 weeks of age by real-time PCR; if positive, birth samples were retrieved where available to distinguish congenital and postnatal infection. The impact of co-infection on mortality through 6 months was estimated using Kaplan-Meier and Cox proportional hazards methods.<h4>Results</h4>At 6 weeks, 203/257 (79%) HIV-infected infants were CMV-positive; 27 (11%) had congenital CMV, 108 (42%) postnatal CMV and 68 (26%) indeterminate timing of infection. By 6 months, 37/108 (34%) infants with postnatal CMV versus 16/54 (30%) CMV-negative infants died (adjusted hazard ratio (aHR) 1.1 [95%CI 0.6, 2.2]). At 6 weeks, 33/257 (13%) HIV-infected infants had EBV co-infection; 6 (2%) had congenital EBV, 18 (7%) postnatal EBV and 9 (4%) indeterminate timing of infection. By 6 months, 5/18 (28%) infants with postnatal EBV versus 72/224 (32%) EBV-negative infants died (aHR 0.8 [95%CI 0.3, 2.3]).<h4>Conclusions</h4>The vast majority of HIV-infants had acquired CMV by 6 weeks, and EBV co-infection occurred earlier than expected, with one in eight HIV-infected infants positive for EBV by 6 weeks. There was a high prevalence of congenital CMV infection and we identified 6 infants with congenital EBV infection, which has not previously been reported in Africa or in the context of HIV infection. Neither CMV nor EBV co-infection was associated with increased mortality.
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spelling doaj-art-e5be80aa902d4387875acec0b9ea6c5e2025-08-20T03:10:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11487010.1371/journal.pone.0114870Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.Hlanai GumboBernard ChasekwaJames A ChurchRobert NtoziniKuda MutasaJean H HumphreyAndrew J Prendergast<h4>Background</h4>HIV-infected infants in sub-Saharan Africa have rapid disease progression. We hypothesized that co-infection with cytomegalovirus (CMV) or Epstein Barr virus (EBV) increases mortality in HIV-infected infants.<h4>Methods</h4>257 antiretroviral therapy-naïve HIV-infected Zimbabwean infants were tested for CMV and EBV at 6 weeks of age by real-time PCR; if positive, birth samples were retrieved where available to distinguish congenital and postnatal infection. The impact of co-infection on mortality through 6 months was estimated using Kaplan-Meier and Cox proportional hazards methods.<h4>Results</h4>At 6 weeks, 203/257 (79%) HIV-infected infants were CMV-positive; 27 (11%) had congenital CMV, 108 (42%) postnatal CMV and 68 (26%) indeterminate timing of infection. By 6 months, 37/108 (34%) infants with postnatal CMV versus 16/54 (30%) CMV-negative infants died (adjusted hazard ratio (aHR) 1.1 [95%CI 0.6, 2.2]). At 6 weeks, 33/257 (13%) HIV-infected infants had EBV co-infection; 6 (2%) had congenital EBV, 18 (7%) postnatal EBV and 9 (4%) indeterminate timing of infection. By 6 months, 5/18 (28%) infants with postnatal EBV versus 72/224 (32%) EBV-negative infants died (aHR 0.8 [95%CI 0.3, 2.3]).<h4>Conclusions</h4>The vast majority of HIV-infants had acquired CMV by 6 weeks, and EBV co-infection occurred earlier than expected, with one in eight HIV-infected infants positive for EBV by 6 weeks. There was a high prevalence of congenital CMV infection and we identified 6 infants with congenital EBV infection, which has not previously been reported in Africa or in the context of HIV infection. Neither CMV nor EBV co-infection was associated with increased mortality.https://doi.org/10.1371/journal.pone.0114870
spellingShingle Hlanai Gumbo
Bernard Chasekwa
James A Church
Robert Ntozini
Kuda Mutasa
Jean H Humphrey
Andrew J Prendergast
Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.
PLoS ONE
title Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.
title_full Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.
title_fullStr Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.
title_full_unstemmed Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.
title_short Congenital and postnatal CMV and EBV acquisition in HIV-infected Zimbabwean infants.
title_sort congenital and postnatal cmv and ebv acquisition in hiv infected zimbabwean infants
url https://doi.org/10.1371/journal.pone.0114870
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