A Quantitative Chemometric Study of Pharmaceutical Tablet Formulations Using Multi-Spectroscopic Fibre Optic Probes
<b>Background/Objectives:</b> Two fibre optic probes were custom designed to perform Raman and near-infrared spectroscopic measurements. Our long-term objective is to develop a non-destructive tool able to collect data in hard-to-access locations for real-time analysis or diagnostic purp...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/17/12/1659 |
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| Summary: | <b>Background/Objectives:</b> Two fibre optic probes were custom designed to perform Raman and near-infrared spectroscopic measurements. Our long-term objective is to develop a non-destructive tool able to collect data in hard-to-access locations for real-time analysis or diagnostic purposes. This study evaluated the quantitative performances of Probe A and Probe B using model pharmaceutical tablets. <b>Methods:</b> Measurements were performed using pharmaceutical tablets containing hydroxyl propylcellulose, titanium dioxide (anatase), lactose monohydrate, and indomethacin (γ form). Material content and thickness of bilayer samples (samples consisting of a top layer and a bottom layer of differing materials) were also assessed using Probe A to evaluate its capabilities to collect sub-surface information. Principal component analysis and partial least squares regression models were using individual and fused data to evaluate the performances of the different probe configurations. <b>Results:</b> Hydroxymethyl cellulose (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.98</mn></mrow></semantics></math></inline-formula>, RMSEP = 2.27% <i>w</i>/<i>w</i>) and lactose monohydrate (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.97</mn></mrow></semantics></math></inline-formula>, RMSEP = 2.96% <i>w</i>/<i>w</i>) content were most effectively estimated by near-infrared spectroscopy data collected using Probe A. Titanium dioxide (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.99</mn></mrow></semantics></math></inline-formula>, RMSEP = 0.21% <i>w</i>/<i>w</i>) content was most effectively estimated using a combination of 785 nm Raman spectroscopy and near-infrared spectroscopy using Probe B. Indomethacin (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.97</mn></mrow></semantics></math></inline-formula>, RMSEP = 1.01% <i>w</i>/<i>w</i>) was best estimated using a low-level fused dataset collected using 0 mm, 2.5 mm, and 5.0 mm lateral offsets of 785 nm spatially offset Raman spectroscopy using Probe A. <b>Conclusions:</b> The different probe configurations were able to reliably collect data and demonstrated robust quantitative performances. These results highlight the advantage of using multiple techniques for analysing different structures. |
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| ISSN: | 1424-8247 |