The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis
ABSTRACT One Health is a unified approach that aims to sustain and maintain the health of humans, animals and the ecosystem. The environment plays an important role in the spread of resistance genes, as it is an unlimited source of antimicrobial resistance genes. Escherichia coli can acquire and spr...
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Wiley
2025-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70209 |
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| author | Didem Karademir Banu Kaskatepe Hilal Basak Erol Suleyman Yalcin Yasemin Numanoglu Cevik |
| author_facet | Didem Karademir Banu Kaskatepe Hilal Basak Erol Suleyman Yalcin Yasemin Numanoglu Cevik |
| author_sort | Didem Karademir |
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| description | ABSTRACT One Health is a unified approach that aims to sustain and maintain the health of humans, animals and the ecosystem. The environment plays an important role in the spread of resistance genes, as it is an unlimited source of antimicrobial resistance genes. Escherichia coli can acquire and spread resistance genes from its environment. Extended‐spectrum beta‐lactamase (ESBL)‐producing E. coli is a global concern because it can hydrolyse many beta‐lactam antibiotics. In this study, the aim was to isolate E. coli from faeces and soil samples collected from cattle, sheep and poultry in three districts (Golbası, Haymana and Cubuk) where livestock (cattle, sheep and poultry) farming activities are intensively carried out. In addition, the antibiotic susceptibility of the isolated E. coli was to be determined using phenotypic and genotypic methods and the presence of ESBLs was to be determined using a double‐disc synergy test. All 120 E. coli isolates were confirmed by the MALDI‐TOF MS method. The resistance rates of all isolates were as follows: ampicillin, 12.5%; trimethoprim/sulfamethoxazole, 6.6%; cefazolin, 0.83%; ciprofloxacin, 2.5%; ceftazidime, 0.83%; cefotaxime, 1.6% and ceftriaxone, 1.6%. Cefazolin (99.1%) and trimethoprim/sulfamethoxazole (0.83%) were determined to have intermediate susceptibility. Only one E. coli strain was found to be ESBL positive via phenotypic methods, and whole‐genome analysis was performed on this strain. As a result of whole‐genome analysis, ESBL‐related CTX‐M‐14 and TEM‐1 genes were found in the plasmids. This is the first study on the determination of antibiotic susceptibility and the presence of ESBL in E. coli isolated from the soil and faeces samples of farms in these regions. More studies are needed to determine and understand antibiotic resistance and ESBL positivity in environmental samples. Therefore, the One Health approach should be emphasised. |
| format | Article |
| id | doaj-art-e5bad54da19045ae875dc305b0d3ecdc |
| institution | OA Journals |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-e5bad54da19045ae875dc305b0d3ecdc2025-08-20T02:30:06ZengWileyVeterinary Medicine and Science2053-10952025-05-01113n/an/a10.1002/vms3.70209The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic AnalysisDidem Karademir0Banu Kaskatepe1Hilal Basak Erol2Suleyman Yalcin3Yasemin Numanoglu Cevik4Department of Pharmaceutical Microbiology Ankara University Graduate School of Health Science Ankara TurkeyDepartment of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy Ankara TurkeyDepartment of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy Ankara TurkeyDepartment of Microbiology Reference Laboratory and Biological Product MoH General Directorate of Public Health Ankara TurkeyDepartment of Microbiology Reference Laboratory and Biological Product MoH General Directorate of Public Health Ankara TurkeyABSTRACT One Health is a unified approach that aims to sustain and maintain the health of humans, animals and the ecosystem. The environment plays an important role in the spread of resistance genes, as it is an unlimited source of antimicrobial resistance genes. Escherichia coli can acquire and spread resistance genes from its environment. Extended‐spectrum beta‐lactamase (ESBL)‐producing E. coli is a global concern because it can hydrolyse many beta‐lactam antibiotics. In this study, the aim was to isolate E. coli from faeces and soil samples collected from cattle, sheep and poultry in three districts (Golbası, Haymana and Cubuk) where livestock (cattle, sheep and poultry) farming activities are intensively carried out. In addition, the antibiotic susceptibility of the isolated E. coli was to be determined using phenotypic and genotypic methods and the presence of ESBLs was to be determined using a double‐disc synergy test. All 120 E. coli isolates were confirmed by the MALDI‐TOF MS method. The resistance rates of all isolates were as follows: ampicillin, 12.5%; trimethoprim/sulfamethoxazole, 6.6%; cefazolin, 0.83%; ciprofloxacin, 2.5%; ceftazidime, 0.83%; cefotaxime, 1.6% and ceftriaxone, 1.6%. Cefazolin (99.1%) and trimethoprim/sulfamethoxazole (0.83%) were determined to have intermediate susceptibility. Only one E. coli strain was found to be ESBL positive via phenotypic methods, and whole‐genome analysis was performed on this strain. As a result of whole‐genome analysis, ESBL‐related CTX‐M‐14 and TEM‐1 genes were found in the plasmids. This is the first study on the determination of antibiotic susceptibility and the presence of ESBL in E. coli isolated from the soil and faeces samples of farms in these regions. More studies are needed to determine and understand antibiotic resistance and ESBL positivity in environmental samples. Therefore, the One Health approach should be emphasised.https://doi.org/10.1002/vms3.70209antibiogramantibiotic resistanceEscherichia coliextended‐spectrum beta lactamaseMALDI‐TOF MSresistance gene |
| spellingShingle | Didem Karademir Banu Kaskatepe Hilal Basak Erol Suleyman Yalcin Yasemin Numanoglu Cevik The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis Veterinary Medicine and Science antibiogram antibiotic resistance Escherichia coli extended‐spectrum beta lactamase MALDI‐TOF MS resistance gene |
| title | The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis |
| title_full | The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis |
| title_fullStr | The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis |
| title_full_unstemmed | The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis |
| title_short | The Study of Escherichia coli as Antimicrobial‐Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI‐TOF MS and Genomic Analysis |
| title_sort | study of escherichia coli as antimicrobial resistant sentinel microorganism isolated in the farms of three districts of ankara by maldi tof ms and genomic analysis |
| topic | antibiogram antibiotic resistance Escherichia coli extended‐spectrum beta lactamase MALDI‐TOF MS resistance gene |
| url | https://doi.org/10.1002/vms3.70209 |
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