Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients
Abstract Pancreatic ductal adenocarcinomas (PDAC) are among the most fatal cancers, in part due to frequent detection at advanced stages. Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), the most sensitive diagnostic method of PDAC in current standard clinical practice, is invasive, co...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-62685-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849332193902460928 |
|---|---|
| author | Travis W. Moore Elias Spiliotopoulos Rowan L. Callahan C. Ward Kirschbaum Conner F. Bailey Hyun Ji Kim Breeshey Roskams-Hieter Florian Goncalves Dove Keith Aaron J. Grossberg Paul T. Spellman Gordon B. Mills Rosalie C. Sears Terry K. Morgan Thuy T. M. Ngo |
| author_facet | Travis W. Moore Elias Spiliotopoulos Rowan L. Callahan C. Ward Kirschbaum Conner F. Bailey Hyun Ji Kim Breeshey Roskams-Hieter Florian Goncalves Dove Keith Aaron J. Grossberg Paul T. Spellman Gordon B. Mills Rosalie C. Sears Terry K. Morgan Thuy T. M. Ngo |
| author_sort | Travis W. Moore |
| collection | DOAJ |
| description | Abstract Pancreatic ductal adenocarcinomas (PDAC) are among the most fatal cancers, in part due to frequent detection at advanced stages. Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), the most sensitive diagnostic method of PDAC in current standard clinical practice, is invasive, costly, with access limited to major healthcare settings. Here, we present a non-invasive evaluation of plasma cell-free RNA (cfRNA) for PDAC detection in pre-diagnostic high-risk and de novo symptomatic patients presenting for EUS-FNA. We develop a cfRNA normalization method to account for preanalytical variation and handling effects and derive 29 potential cfRNA biomarkers for PDAC diagnosis using 153 samples collected prior to the EUS procedure. Biomarkers related to liver function are elevated in PDAC samples, including early-stage patients without liver metastasis. Classification of PDAC using these biomarkers is validated using an independent cohort of 95 samples. Our findings could help to improve diagnostic utility in high-risk and symptomatic individuals. |
| format | Article |
| id | doaj-art-e5b54916f7af4efaba3460a6ae0e7138 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e5b54916f7af4efaba3460a6ae0e71382025-08-20T03:46:17ZengNature PortfolioNature Communications2041-17232025-08-0116111210.1038/s41467-025-62685-yCell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patientsTravis W. Moore0Elias Spiliotopoulos1Rowan L. Callahan2C. Ward Kirschbaum3Conner F. Bailey4Hyun Ji Kim5Breeshey Roskams-Hieter6Florian Goncalves7Dove Keith8Aaron J. Grossberg9Paul T. Spellman10Gordon B. Mills11Rosalie C. Sears12Terry K. Morgan13Thuy T. M. Ngo14Cancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityBrenden-Colson Center for Pancreatic Care, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityDivision of Oncological Sciences, Knight Cancer Institute, Oregon Health and Science UniversityBrenden-Colson Center for Pancreatic Care, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityCancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health and Science UniversityAbstract Pancreatic ductal adenocarcinomas (PDAC) are among the most fatal cancers, in part due to frequent detection at advanced stages. Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), the most sensitive diagnostic method of PDAC in current standard clinical practice, is invasive, costly, with access limited to major healthcare settings. Here, we present a non-invasive evaluation of plasma cell-free RNA (cfRNA) for PDAC detection in pre-diagnostic high-risk and de novo symptomatic patients presenting for EUS-FNA. We develop a cfRNA normalization method to account for preanalytical variation and handling effects and derive 29 potential cfRNA biomarkers for PDAC diagnosis using 153 samples collected prior to the EUS procedure. Biomarkers related to liver function are elevated in PDAC samples, including early-stage patients without liver metastasis. Classification of PDAC using these biomarkers is validated using an independent cohort of 95 samples. Our findings could help to improve diagnostic utility in high-risk and symptomatic individuals.https://doi.org/10.1038/s41467-025-62685-y |
| spellingShingle | Travis W. Moore Elias Spiliotopoulos Rowan L. Callahan C. Ward Kirschbaum Conner F. Bailey Hyun Ji Kim Breeshey Roskams-Hieter Florian Goncalves Dove Keith Aaron J. Grossberg Paul T. Spellman Gordon B. Mills Rosalie C. Sears Terry K. Morgan Thuy T. M. Ngo Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients Nature Communications |
| title | Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients |
| title_full | Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients |
| title_fullStr | Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients |
| title_full_unstemmed | Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients |
| title_short | Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients |
| title_sort | cell free rna detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients |
| url | https://doi.org/10.1038/s41467-025-62685-y |
| work_keys_str_mv | AT traviswmoore cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT eliasspiliotopoulos cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT rowanlcallahan cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT cwardkirschbaum cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT connerfbailey cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT hyunjikim cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT breesheyroskamshieter cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT floriangoncalves cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT dovekeith cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT aaronjgrossberg cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT paultspellman cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT gordonbmills cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT rosaliecsears cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT terrykmorgan cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients AT thuytmngo cellfreernadetectionofpancreaticcancerinprediagnostichighriskandsymptomaticpatients |