Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch

To investigate the effects of mechanical toughening and toughening-enzymatic modification on the formation, structure, and physicochemical properties of slow-digestible starch content in sorghum starch, a comparative analysis was conducted on the structure and physicochemical characteristics of sorg...

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Main Authors: Hongda MA, Jiaxin YAO, Shijie LIU, Yashi WANG, Zhijiang LI, Hongwei ZHANG
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-08-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090396
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author Hongda MA
Jiaxin YAO
Shijie LIU
Yashi WANG
Zhijiang LI
Hongwei ZHANG
author_facet Hongda MA
Jiaxin YAO
Shijie LIU
Yashi WANG
Zhijiang LI
Hongwei ZHANG
author_sort Hongda MA
collection DOAJ
description To investigate the effects of mechanical toughening and toughening-enzymatic modification on the formation, structure, and physicochemical properties of slow-digestible starch content in sorghum starch, a comparative analysis was conducted on the structure and physicochemical characteristics of sorghum starch before and after these modifications. Parameters including slow-digestible starch content, starch granule morphology, crystal structure, solubility, expansion capacity, water extraction rate, and thermal stability were examined. Results showed that, the slow-digestible starch content in modified sorghum starch significantly increased (P<0.05). Specifically, the content after toughening reached 18.68%, and following toughening and enzyme modification, it increased to 38.37%. The surface morphology of enzyme-modified sorghum starch exhibited irregularities and fragmentation, and the internal structure of modified sorghum starch underwent rearrangement. Notably, the relative crystallinity of toughened sorghum starch increased to 21.43%, and that of enzymatically modified toughened sorghum starch increased to 27.53%. Toughened sorghum starch retained an A-type crystalline form, while enzymatically modified toughened sorghum starch transitioned to an A+V type crystalline form. At 90 °C, both solubility and dilatability were significantly lower in modified sorghum starch compared to unmodified samples (P<0.05), while transparency and water precipitation rates were markedly higher (P<0.05). An increase in the initial gelatinization temperature was observed, indicating enhanced thermal stability. In conclusion, compared to toughened modified sorghum starch alone, the combination of toughening and enzyme modification demonstrated a more pronounced impact on the content, structural attributes, and physicochemical properties of slow-digestible starch in starchy sorghum materials.
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publisher The editorial department of Science and Technology of Food Industry
record_format Article
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spelling doaj-art-e5af84baef664507a0e4afe44a48af882025-08-20T03:43:55ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-014616111810.13386/j.issn1002-0306.20240903962024090396-16Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum StarchHongda MA0Jiaxin YAO1Shijie LIU2Yashi WANG3Zhijiang LI4Hongwei ZHANG5College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaTo investigate the effects of mechanical toughening and toughening-enzymatic modification on the formation, structure, and physicochemical properties of slow-digestible starch content in sorghum starch, a comparative analysis was conducted on the structure and physicochemical characteristics of sorghum starch before and after these modifications. Parameters including slow-digestible starch content, starch granule morphology, crystal structure, solubility, expansion capacity, water extraction rate, and thermal stability were examined. Results showed that, the slow-digestible starch content in modified sorghum starch significantly increased (P<0.05). Specifically, the content after toughening reached 18.68%, and following toughening and enzyme modification, it increased to 38.37%. The surface morphology of enzyme-modified sorghum starch exhibited irregularities and fragmentation, and the internal structure of modified sorghum starch underwent rearrangement. Notably, the relative crystallinity of toughened sorghum starch increased to 21.43%, and that of enzymatically modified toughened sorghum starch increased to 27.53%. Toughened sorghum starch retained an A-type crystalline form, while enzymatically modified toughened sorghum starch transitioned to an A+V type crystalline form. At 90 °C, both solubility and dilatability were significantly lower in modified sorghum starch compared to unmodified samples (P<0.05), while transparency and water precipitation rates were markedly higher (P<0.05). An increase in the initial gelatinization temperature was observed, indicating enhanced thermal stability. In conclusion, compared to toughened modified sorghum starch alone, the combination of toughening and enzyme modification demonstrated a more pronounced impact on the content, structural attributes, and physicochemical properties of slow-digestible starch in starchy sorghum materials.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090396sorghum starchslow-digestible starchtoughening-enzyme modificationstructural attributesphysicochemical properties
spellingShingle Hongda MA
Jiaxin YAO
Shijie LIU
Yashi WANG
Zhijiang LI
Hongwei ZHANG
Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch
Shipin gongye ke-ji
sorghum starch
slow-digestible starch
toughening-enzyme modification
structural attributes
physicochemical properties
title Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch
title_full Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch
title_fullStr Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch
title_full_unstemmed Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch
title_short Effect of Toughening-Enzyme Modification on the Structure and Properties of Sorghum Starch
title_sort effect of toughening enzyme modification on the structure and properties of sorghum starch
topic sorghum starch
slow-digestible starch
toughening-enzyme modification
structural attributes
physicochemical properties
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090396
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