Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate
Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. s...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2017/9392532 |
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| author | Ana Carla Guidini Valentini Gheller Jacqueline Kerkhoff Gerardo Magela Vieira Júnior Kleber Eduardo de Campos Marina Mariko Sugui |
| author_facet | Ana Carla Guidini Valentini Gheller Jacqueline Kerkhoff Gerardo Magela Vieira Júnior Kleber Eduardo de Campos Marina Mariko Sugui |
| author_sort | Ana Carla Guidini Valentini Gheller |
| collection | DOAJ |
| description | Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis. |
| format | Article |
| id | doaj-art-e5adfb8ec4e9406e9c61097c0a238c04 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e5adfb8ec4e9406e9c61097c0a238c042025-02-03T01:24:14ZengWileyThe Scientific World Journal2356-61401537-744X2017-01-01201710.1155/2017/93925329392532Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium GlutamateAna Carla Guidini Valentini Gheller0Jacqueline Kerkhoff1Gerardo Magela Vieira Júnior2Kleber Eduardo de Campos3Marina Mariko Sugui4Institute of Natural, Humanities and Social Sciences, Federal University of Mato Grosso, Sinop, MT, BrazilInstitute of Natural, Humanities and Social Sciences, Federal University of Mato Grosso, Sinop, MT, BrazilDepartment of Chemistry, Federal University of Piauí, Teresina, PI, BrazilInstitute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, BrazilInstitute of Health Sciences, Federal University of Mato Grosso, Sinop, MT, BrazilHibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.http://dx.doi.org/10.1155/2017/9392532 |
| spellingShingle | Ana Carla Guidini Valentini Gheller Jacqueline Kerkhoff Gerardo Magela Vieira Júnior Kleber Eduardo de Campos Marina Mariko Sugui Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate The Scientific World Journal |
| title | Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate |
| title_full | Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate |
| title_fullStr | Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate |
| title_full_unstemmed | Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate |
| title_short | Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate |
| title_sort | antimutagenic effect of hibiscus sabdariffa l aqueous extract on rats treated with monosodium glutamate |
| url | http://dx.doi.org/10.1155/2017/9392532 |
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