Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC

<b>Objectives</b>: This study explores the development and evaluation of a bilayer sustained-release (SR) tablet formulation of ruxolitinib. As a BCS Class 1 drug, ruxolitinib requires twice-daily dosing due to its short half-life. We designed a bilayer tablet that integrates immediate-r...

Full description

Saved in:
Bibliographic Details
Main Authors: Namhyuck Kim, Kyoungho Kim, Seungwei Jeong, Jiyeong Kim, Helen Cho, Young-Joo Lee, Sangyeob Park
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Pharmaceutics
Subjects:
Online Access:https://www.mdpi.com/1999-4923/17/4/432
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850144097667383296
author Namhyuck Kim
Kyoungho Kim
Seungwei Jeong
Jiyeong Kim
Helen Cho
Young-Joo Lee
Sangyeob Park
author_facet Namhyuck Kim
Kyoungho Kim
Seungwei Jeong
Jiyeong Kim
Helen Cho
Young-Joo Lee
Sangyeob Park
author_sort Namhyuck Kim
collection DOAJ
description <b>Objectives</b>: This study explores the development and evaluation of a bilayer sustained-release (SR) tablet formulation of ruxolitinib. As a BCS Class 1 drug, ruxolitinib requires twice-daily dosing due to its short half-life. We designed a bilayer tablet that integrates immediate-release (IR) and SR components in varying ratios to achieve sustained plasma concentrations, which we evaluated using discriminative analysis. <b>Methods</b>: Bilayer tablets combining IR and SR components were prepared in different ratios. In vitro dissolution tests and pharmacokinetic studies were conducted using Beagle dogs, followed by the evaluation of in vivo–in vitro correlation (IVIVC), along with a discriminative pharmacokinetic analysis focused on the SR layer. <b>Results</b>: A discriminative pharmacokinetic and IVIVC analysis was applied to all bilayer tablets, offering clearer insights into the plasma concentration and dissolution profiles. Pharmacokinetic studies showed that test formulation F4, which has a 20:20 IR-to-SR ratio, is expected to provide a similar area under the curve (AUC) while prolonging exposure compared to the reference IR tablet. <b>Conclusions</b>: This study highlights the potential of a bilayer tablet approach, combined with discriminative pharmacokinetic and IVIVC analysis, for creating a sustained-release dosage form of ruxolitinib.
format Article
id doaj-art-e5ad111f1b3a44e499d0acf8d87b6bbd
institution OA Journals
issn 1999-4923
language English
publishDate 2025-03-01
publisher MDPI AG
record_format Article
series Pharmaceutics
spelling doaj-art-e5ad111f1b3a44e499d0acf8d87b6bbd2025-08-20T02:28:28ZengMDPI AGPharmaceutics1999-49232025-03-0117443210.3390/pharmaceutics17040432Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVCNamhyuck Kim0Kyoungho Kim1Seungwei Jeong2Jiyeong Kim3Helen Cho4Young-Joo Lee5Sangyeob Park6Samyang Holdings Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Republic of KoreaSamyang Holdings Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Republic of KoreaSamyang Holdings Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Republic of KoreaSamyang Holdings Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Republic of KoreaSamyang Holdings Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Republic of KoreaDivision of Biopharmaceutics, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 02453, Republic of KoreaSamyang Holdings Corp., 295 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Republic of Korea<b>Objectives</b>: This study explores the development and evaluation of a bilayer sustained-release (SR) tablet formulation of ruxolitinib. As a BCS Class 1 drug, ruxolitinib requires twice-daily dosing due to its short half-life. We designed a bilayer tablet that integrates immediate-release (IR) and SR components in varying ratios to achieve sustained plasma concentrations, which we evaluated using discriminative analysis. <b>Methods</b>: Bilayer tablets combining IR and SR components were prepared in different ratios. In vitro dissolution tests and pharmacokinetic studies were conducted using Beagle dogs, followed by the evaluation of in vivo–in vitro correlation (IVIVC), along with a discriminative pharmacokinetic analysis focused on the SR layer. <b>Results</b>: A discriminative pharmacokinetic and IVIVC analysis was applied to all bilayer tablets, offering clearer insights into the plasma concentration and dissolution profiles. Pharmacokinetic studies showed that test formulation F4, which has a 20:20 IR-to-SR ratio, is expected to provide a similar area under the curve (AUC) while prolonging exposure compared to the reference IR tablet. <b>Conclusions</b>: This study highlights the potential of a bilayer tablet approach, combined with discriminative pharmacokinetic and IVIVC analysis, for creating a sustained-release dosage form of ruxolitinib.https://www.mdpi.com/1999-4923/17/4/432bilayer tabletsustained release formulationruxolitinibIVIVCdissolution
spellingShingle Namhyuck Kim
Kyoungho Kim
Seungwei Jeong
Jiyeong Kim
Helen Cho
Young-Joo Lee
Sangyeob Park
Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC
Pharmaceutics
bilayer tablet
sustained release formulation
ruxolitinib
IVIVC
dissolution
title Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC
title_full Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC
title_fullStr Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC
title_full_unstemmed Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC
title_short Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC
title_sort development and evaluation of bilayer sustained release tablets of ruxolitinib using discriminative pharmacokinetic analysis and ivivc
topic bilayer tablet
sustained release formulation
ruxolitinib
IVIVC
dissolution
url https://www.mdpi.com/1999-4923/17/4/432
work_keys_str_mv AT namhyuckkim developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc
AT kyounghokim developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc
AT seungweijeong developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc
AT jiyeongkim developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc
AT helencho developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc
AT youngjoolee developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc
AT sangyeobpark developmentandevaluationofbilayersustainedreleasetabletsofruxolitinibusingdiscriminativepharmacokineticanalysisandivivc