Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study

Background. Screening and termination of pregnancy (TOP) for Down syndrome (DS) are both available in South Africa (SA), but DS is infrequently diagnosed prenatally in the public sector (7% in 2008), resulting in a high live-birth prevalence (1.33 - 2.1 per 1 000). In the SA public sector, DS s...

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Main Authors: L Geerts, N Du Toit, M Schoeman
Format: Article
Language:English
Published: South African Medical Association 2025-08-01
Series:South African Medical Journal
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Online Access:https://samajournals.co.za/index.php/samj/article/view/2052
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author L Geerts
N Du Toit
M Schoeman
author_facet L Geerts
N Du Toit
M Schoeman
author_sort L Geerts
collection DOAJ
description Background. Screening and termination of pregnancy (TOP) for Down syndrome (DS) are both available in South Africa (SA), but DS is infrequently diagnosed prenatally in the public sector (7% in 2008), resulting in a high live-birth prevalence (1.33 - 2.1 per 1 000). In the SA public sector, DS screening and confirmatory genetic testing are fully state subsidised for women of advanced maternal age (AMA) but, owing to the low positive predictive value of AMA-based screening, ultrasound-based screening is also offered. Given the limited resources and the steady increase in the number of pregnant women of AMA, the value of DS screening in altering pregnancy outcome needs to be critically assessed. Objectives. To determine the uptake of prenatal screening for DS, invasive testing and TOP in pregnant women of AMA, as well as factors influencing maternal decisions. Methods. This retrospective cohort study, based on prospectively captured data, includes all women of AMA (>37 years at conception) seen at a regional fetal medicine unit in Cape Town offering fully state subsidised DS screening and testing for a geographically defined area, including mostly women of African or mixed ancestry. Screening was age- and ultrasound-based, and DS risks were calculated using published algorithms. Non-directive genetic counselling was provided to all women ≥40 years old (pre-screen if feasible), women with a relevant history, a fetal anomaly or DS risk higher than that of a woman aged 37 years. Participant characteristics, results, decisions and reasons to decline testing were recorded prospectively, and compared between women <40 completed years and ≥40 years old, and between women accepting or declining invasive testing or TOP. Results. During the study period, 1 196 women of AMA were seen. Ninety-three received pre-screen counselling, and 44 of these declined DS screening (47.3% (95% confidence interval (CI) 36.9 - 57.9)). Uptake of invasive testing after screening was low (18.1% (CI 15.2 - 21.3)). Age category was not an independent confounder for this, but uptake was lower after previous miscarriage(s), higher after high-risk screening results and highest with a fetal anomaly. The most common reason for declining testing was opposition to TOP. The uptake of TOP for DS, when offered to those who were screened and had accepted invasive testing, was 65.8% (48.7 - 80.4). Conclusion. The uptake of screening and/or testing was low, and this reflected strong views on TOP for DS. As uptake of testing and/or TOP was higher with abnormal ultrasound findings, a prenatal screening programme addressing structural anomalies and aneuploidies simultaneously (i.e. ultrasound) is preferred over other DS screening tools that target DS specifically.
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spelling doaj-art-e5aa275fa6f04180afe520b88081167c2025-08-20T03:59:32ZengSouth African Medical AssociationSouth African Medical Journal0256-95742078-51352025-08-01115710.7196/SAMJ.2025.v115i7.2052Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort studyL Geerts0N Du Toit1M Schoeman2Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Academic Hospital, Cape Town, South AfricaDepartment of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Academic Hospital, Cape Town, South AfricaDivision of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Academic Hospital, Cape Town, South Africa Background. Screening and termination of pregnancy (TOP) for Down syndrome (DS) are both available in South Africa (SA), but DS is infrequently diagnosed prenatally in the public sector (7% in 2008), resulting in a high live-birth prevalence (1.33 - 2.1 per 1 000). In the SA public sector, DS screening and confirmatory genetic testing are fully state subsidised for women of advanced maternal age (AMA) but, owing to the low positive predictive value of AMA-based screening, ultrasound-based screening is also offered. Given the limited resources and the steady increase in the number of pregnant women of AMA, the value of DS screening in altering pregnancy outcome needs to be critically assessed. Objectives. To determine the uptake of prenatal screening for DS, invasive testing and TOP in pregnant women of AMA, as well as factors influencing maternal decisions. Methods. This retrospective cohort study, based on prospectively captured data, includes all women of AMA (>37 years at conception) seen at a regional fetal medicine unit in Cape Town offering fully state subsidised DS screening and testing for a geographically defined area, including mostly women of African or mixed ancestry. Screening was age- and ultrasound-based, and DS risks were calculated using published algorithms. Non-directive genetic counselling was provided to all women ≥40 years old (pre-screen if feasible), women with a relevant history, a fetal anomaly or DS risk higher than that of a woman aged 37 years. Participant characteristics, results, decisions and reasons to decline testing were recorded prospectively, and compared between women <40 completed years and ≥40 years old, and between women accepting or declining invasive testing or TOP. Results. During the study period, 1 196 women of AMA were seen. Ninety-three received pre-screen counselling, and 44 of these declined DS screening (47.3% (95% confidence interval (CI) 36.9 - 57.9)). Uptake of invasive testing after screening was low (18.1% (CI 15.2 - 21.3)). Age category was not an independent confounder for this, but uptake was lower after previous miscarriage(s), higher after high-risk screening results and highest with a fetal anomaly. The most common reason for declining testing was opposition to TOP. The uptake of TOP for DS, when offered to those who were screened and had accepted invasive testing, was 65.8% (48.7 - 80.4). Conclusion. The uptake of screening and/or testing was low, and this reflected strong views on TOP for DS. As uptake of testing and/or TOP was higher with abnormal ultrasound findings, a prenatal screening programme addressing structural anomalies and aneuploidies simultaneously (i.e. ultrasound) is preferred over other DS screening tools that target DS specifically. https://samajournals.co.za/index.php/samj/article/view/2052Down syndromeAneuploidyPrenatal screeningLMICAfricaultrasound
spellingShingle L Geerts
N Du Toit
M Schoeman
Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study
South African Medical Journal
Down syndrome
Aneuploidy
Prenatal screening
LMIC
Africa
ultrasound
title Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study
title_full Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study
title_fullStr Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study
title_full_unstemmed Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study
title_short Aneuploidy screening in women of advanced age in the public healthcare setting of a low- to middle- income country – an observational cohort study
title_sort aneuploidy screening in women of advanced age in the public healthcare setting of a low to middle income country an observational cohort study
topic Down syndrome
Aneuploidy
Prenatal screening
LMIC
Africa
ultrasound
url https://samajournals.co.za/index.php/samj/article/view/2052
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