Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process
<b>Background/Objectives:</b> The primary goal of our research is to develop a process to prepare an aqueous dispersion of Cisplatin, an important anticancer drug, with increased solubility and storage stability. <b>Method:</b> In this context, we report the use of a customiz...
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MDPI AG
2024-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/16/11/1471 |
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| author | Sudhir Kumar Sharma Loganathan Palanikumar Renu Pasricha Thirumurugan Prakasam Mazin Magzoub Ramesh Jagannathan |
| author_facet | Sudhir Kumar Sharma Loganathan Palanikumar Renu Pasricha Thirumurugan Prakasam Mazin Magzoub Ramesh Jagannathan |
| author_sort | Sudhir Kumar Sharma |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> The primary goal of our research is to develop a process to prepare an aqueous dispersion of Cisplatin, an important anticancer drug, with increased solubility and storage stability. <b>Method:</b> In this context, we report the use of a customized RESS process for the synthesis of a novel, amber-colored and viscous aqueous cisplatin solution, an important anticancer drug, which we have denoted as “liquid” cisplatin. <b>Results:</b> Using specialized liquid cell in situ transmission electron microscopy (Liquid in situ TEM) and Raman spectroscopy, we demonstrated that “liquid” cisplatin comprises a bi-modal distribution of a highly solvated network of stable cisplatin nanoclusters in water and exhibited 27 times greater water solubility than standard cisplatin. More importantly, “liquid” cisplatin was stable at ambient conditions for over two years. Extensive analytical characterization of “liquid” cisplatin confirmed that it retained the original chemical identity of cisplatin. Cell viability and apoptosis studies on human lung adenocarcinoma A549 cells provided compelling evidence that “liquid” cisplatin demonstrated a more sustained anticancer effect compared to standard cisplatin. <b>Conclusions:</b> Aqueous cisplatin solubility was increased by 27X in the “liquid” cisplatin medium which retained its bio efficacy over a 2-year period. Our experimental results suggest the possibility of developing non-invasive and highly effective novel cisplatin drug-delivery platforms. |
| format | Article |
| id | doaj-art-e5a91634cce44c18b0130db59e558f0d |
| institution | DOAJ |
| issn | 1999-4923 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj-art-e5a91634cce44c18b0130db59e558f0d2025-08-20T02:48:07ZengMDPI AGPharmaceutics1999-49232024-11-011611147110.3390/pharmaceutics16111471Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS ProcessSudhir Kumar Sharma0Loganathan Palanikumar1Renu Pasricha2Thirumurugan Prakasam3Mazin Magzoub4Ramesh Jagannathan5Engineering Division, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab EmiratesBiology Program, Division of Science, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab EmiratesRetired from Core Technology Platform, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab EmiratesScience Division, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab EmiratesBiology Program, Division of Science, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab EmiratesEngineering Division, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab Emirates<b>Background/Objectives:</b> The primary goal of our research is to develop a process to prepare an aqueous dispersion of Cisplatin, an important anticancer drug, with increased solubility and storage stability. <b>Method:</b> In this context, we report the use of a customized RESS process for the synthesis of a novel, amber-colored and viscous aqueous cisplatin solution, an important anticancer drug, which we have denoted as “liquid” cisplatin. <b>Results:</b> Using specialized liquid cell in situ transmission electron microscopy (Liquid in situ TEM) and Raman spectroscopy, we demonstrated that “liquid” cisplatin comprises a bi-modal distribution of a highly solvated network of stable cisplatin nanoclusters in water and exhibited 27 times greater water solubility than standard cisplatin. More importantly, “liquid” cisplatin was stable at ambient conditions for over two years. Extensive analytical characterization of “liquid” cisplatin confirmed that it retained the original chemical identity of cisplatin. Cell viability and apoptosis studies on human lung adenocarcinoma A549 cells provided compelling evidence that “liquid” cisplatin demonstrated a more sustained anticancer effect compared to standard cisplatin. <b>Conclusions:</b> Aqueous cisplatin solubility was increased by 27X in the “liquid” cisplatin medium which retained its bio efficacy over a 2-year period. Our experimental results suggest the possibility of developing non-invasive and highly effective novel cisplatin drug-delivery platforms.https://www.mdpi.com/1999-4923/16/11/1471supercritical CO<sub>2</sub> processingRESS techniqueliquid in situ transmission electron microscopycell viabilityapoptosis |
| spellingShingle | Sudhir Kumar Sharma Loganathan Palanikumar Renu Pasricha Thirumurugan Prakasam Mazin Magzoub Ramesh Jagannathan Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process Pharmaceutics supercritical CO<sub>2</sub> processing RESS technique liquid in situ transmission electron microscopy cell viability apoptosis |
| title | Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process |
| title_full | Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process |
| title_fullStr | Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process |
| title_full_unstemmed | Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process |
| title_short | Synthesis of Self-Assembled Nanostructured Cisplatin Using the RESS Process |
| title_sort | synthesis of self assembled nanostructured cisplatin using the ress process |
| topic | supercritical CO<sub>2</sub> processing RESS technique liquid in situ transmission electron microscopy cell viability apoptosis |
| url | https://www.mdpi.com/1999-4923/16/11/1471 |
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